Pentose Phosphate Pathway Flashcards
Detoxification pathways is primarily in what organ?
Liver
Pathways that require NADPH: Detoxification
2 total
- Reduction of Oxidized glutathione (contains sulfur group)
- Cytochrome P450 monooxygenase
What is the directionality of the Phase 2?
BI-directionality
Major role of NADPH in RBC?
To Reduce the DISULFIDE form of Glutathione to the Sulfhydryl form
Purpose of Phase 1?
Oxidative generation of NADPH
Phase 1 is regulated by what enzyme?
G6P Dehydrogenase
What is a key role in determining the fate of the G6P
Cytoplasmic [NADP+]
Components of Glutathione
3 components
- Gamma glutamate
- Cysteine
- Glycine
(GCG)
What is the directionality of the Phase 1?
UNI-directional
Which enzyme had a prosthetic group?
What is the prosthetic?
TransKetolase
Tightly bound by a THIAMINE Pyrophosphate
What two enzymes are used in the Non-oxidative branch?
Transketolase
And
Transaldolase
Phase 2 is the _____ branch of the PPP
Non-oxidative
What disease can G6P Dehydrogenase deficiency cause?
Hemolytic anemia
Overview of steps in Phase 1
G6P ———>
Ribulose 5-Phosphate
What substrate is metabolized by both the Glycolytic and the PP Pathway?
G6P
Phase 1 is the _____ branch of the PPP.
Oxidative
Phase 2 products of the PPP?
Intermediates of GLYCOLYSIS
GAP
&
F6P
Pathways that require NADPH: Synthesis
4 total Biosynthesis
FA
Cholesterol
NT (Nitric Oxide)
Nucleotide
CNN Fake (News)
Calvin cycle
Vs
PPP
- Begins with the fixation of CO2
- Proceeds to use NADPH in the synthesis of GLUCOSE
- Begins with the oxidation of glucose derived Carbon ——> CO2
- Concomitantly generates NADPH
Purpose of Phase 2?
Nonoxidative interconversion of SUGARS
How are Glycolysis and PPP linked?
By 2 Enzymes
Transketolase and Transaldolase
What enzyme requires NADPH to function?
Glutathione Reductase
Diff b/w Transketolase and Transaldolase?
TKL transfers a TWO carbon unit
Whereas,
TAL transfers a THREE carbon unit
What does the PPP create?
Pentose sugars and NADPH
