PCR Flashcards

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1
Q

What does PCR stand for?

A

Polymerase Chain Reaction

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2
Q

What is needed for PCR?

A
  • Taq DNA polymerase
  • DNA nucleotides
  • RNA primers

(Basically, everything you need for replication, but the polymerase is TAQ specifically)

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3
Q

Characteristic feature of Taq polymerase:

A

Does not denature at high temperatures (50-80 degrees Celsius)

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4
Q

Use of PCR:

A

Rapid production of multiple copies of DNA

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5
Q

Stages of PCR:

A

1) Denaturation
2) Annealing
3) Elongation

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6
Q

Stages of PCR:

A

1) Denaturation (strands separate)
2) Annealing (RNA primers attatch)
3) Elongation (Taq DNA polymerase adds DNA nucleotides)

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