PARTS OF MICROSCOPE AND THEIR FUNCTION Flashcards
Magnify image and allow visualization of greater details than is possible with the unaided eye
Light Microscopy
microscope with one lense
Simple Microscope
magnifying glass or pair of reading glasses
Simplest
multiple lense
Compound Microscope
Proper Handling of Microscope
* make sure the ____ is secure
* support the base with __________
* lift with the ____ of the scope
*Do not _____
cord
one hand
arm
swing
The microscope must not be close to the ________
edge
the power of the microscope to enlarge the image of an object
Magnification
The ability of the microscope to distinguish two object from each other
resolution
2 major parts of compound microscope
Optical, Mechanical parts
magnifies the tissue specimens
optical parts
the ocular or eyepiec lens that one looks into
optical part
the objective lens or the lens closest to the tissue sample/slide
optical part
suppoerts the optical parts
mechanical parts
ocular/eyepiece lens alone can magnify with a spec. of _____
10x
4 objective lenses
Scanner 4x
Low Power Objective 10x
High Power Objective 40x
Oil Immersion Objective 100x
Magnification formula
objective magnification x eyepiece magnification
holds the different objective lenses
it allows the rotation of the lenses while viewing
Revolving Nosepiece
supports the lenses and nosepiece of the microscope and connects to the base of the microscope
arm
a platform that is flat used for placing the slides under observation
stage and stage clips
helps in controlling the amount of light that is passing through the opening of the stage
Iris Diaphragm
gathers light from the microscope’s light source and concentrate it into a cone of light that illuminates the specimen
condenser
lowers or raise the stage; used for focus on scanning; usually the low power lens is used enabling the movement of the tube
course adjustment knobs
used for focusing on high power and oil objectives. Moves the body tube for focusing the high-power lens
Fine adjustment knobs
moves the stage sideways
stage controls
Focusing Specimens
1. Always start with the ______ objective
scanning
Focusing Specimens
2. Place your specimen in the _____ of your field of view
center
Focusing SPecimens
3. Use the ____ knob to focus
Coarse
Focusing Specimens
4. Next, use the ____ Knob until clear
Fine
Focusing Specimens
5. Once you’ve focused on Scanning, switch to ________________ objective using the revolving nosepiece
Low Power
Focusing Specimens
6. Repeat steps _______
3-4
Focusing Specimens
7. Now, switch to ____ Power
High
Focusing Specimens
8. At this point, only use the _________________ to focus specimens
fine adjustment knob
Using High Power
1. Rotate to _______ objective, locate desired portion of specimen in the center of the field. (Do not alter _____ for the following steps)
40x; focus
Using High Power
2. ________ rotate so that 40x and 100x objectives straddle the specimen
Partially
Using High Power
3. Place a small drop of ____ on the slide in the center of the lighted area
oil
Using High Power
4. Put the small drop of oil directly over the area of the _________ to be examined.
specimen
Using High Power
5. Rotate so that the ____ oil immersion objective touches the oil and clicks into place
100x
Using High Power
6. Focus only with_____ focus. Hopefully, the specimen will come into focus easily
fine
when you have finished for the day, wipe the 100x oil immersion objective carefully with _______ to remove all oil.
lens paper
_________ stage should any oil spilled on it
cleanse
REcap the immersion oil _______ securely, replace in drawer
container
