PARTS OF MICROSCOPE AND THEIR FUNCTION Flashcards

1
Q

Magnify image and allow visualization of greater details than is possible with the unaided eye

A

Light Microscopy

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2
Q

microscope with one lense

A

Simple Microscope

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3
Q

magnifying glass or pair of reading glasses

A

Simplest

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4
Q

multiple lense

A

Compound Microscope

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5
Q

Proper Handling of Microscope
* make sure the ____ is secure
* support the base with __________
* lift with the ____ of the scope
*Do not _____

A

cord
one hand
arm
swing

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6
Q

The microscope must not be close to the ________

A

edge

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7
Q

the power of the microscope to enlarge the image of an object

A

Magnification

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8
Q

The ability of the microscope to distinguish two object from each other

A

resolution

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9
Q

2 major parts of compound microscope

A

Optical, Mechanical parts

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10
Q

magnifies the tissue specimens

A

optical parts

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11
Q

the ocular or eyepiec lens that one looks into

A

optical part

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12
Q

the objective lens or the lens closest to the tissue sample/slide

A

optical part

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13
Q

suppoerts the optical parts

A

mechanical parts

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14
Q

ocular/eyepiece lens alone can magnify with a spec. of _____

A

10x

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15
Q

4 objective lenses

A

Scanner 4x
Low Power Objective 10x
High Power Objective 40x
Oil Immersion Objective 100x

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16
Q

Magnification formula

A

objective magnification x eyepiece magnification

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17
Q

holds the different objective lenses
it allows the rotation of the lenses while viewing

A

Revolving Nosepiece

18
Q

supports the lenses and nosepiece of the microscope and connects to the base of the microscope

A

arm

19
Q

a platform that is flat used for placing the slides under observation

A

stage and stage clips

20
Q

helps in controlling the amount of light that is passing through the opening of the stage

A

Iris Diaphragm

21
Q

gathers light from the microscope’s light source and concentrate it into a cone of light that illuminates the specimen

A

condenser

22
Q

lowers or raise the stage; used for focus on scanning; usually the low power lens is used enabling the movement of the tube

A

course adjustment knobs

23
Q

used for focusing on high power and oil objectives. Moves the body tube for focusing the high-power lens

A

Fine adjustment knobs

24
Q

moves the stage sideways

A

stage controls

25
Q

Focusing Specimens
1. Always start with the ______ objective

A

scanning

26
Q

Focusing Specimens
2. Place your specimen in the _____ of your field of view

A

center

27
Q

Focusing SPecimens
3. Use the ____ knob to focus

A

Coarse

28
Q

Focusing Specimens
4. Next, use the ____ Knob until clear

A

Fine

29
Q

Focusing Specimens
5. Once you’ve focused on Scanning, switch to ________________ objective using the revolving nosepiece

A

Low Power

30
Q

Focusing Specimens
6. Repeat steps _______

A

3-4

31
Q

Focusing Specimens
7. Now, switch to ____ Power

A

High

32
Q

Focusing Specimens
8. At this point, only use the _________________ to focus specimens

A

fine adjustment knob

33
Q

Using High Power
1. Rotate to _______ objective, locate desired portion of specimen in the center of the field. (Do not alter _____ for the following steps)

A

40x; focus

34
Q

Using High Power
2. ________ rotate so that 40x and 100x objectives straddle the specimen

A

Partially

35
Q

Using High Power
3. Place a small drop of ____ on the slide in the center of the lighted area

A

oil

36
Q

Using High Power
4. Put the small drop of oil directly over the area of the _________ to be examined.

A

specimen

37
Q

Using High Power
5. Rotate so that the ____ oil immersion objective touches the oil and clicks into place

A

100x

38
Q

Using High Power
6. Focus only with_____ focus. Hopefully, the specimen will come into focus easily

A

fine

39
Q

when you have finished for the day, wipe the 100x oil immersion objective carefully with _______ to remove all oil.

A

lens paper

40
Q

_________ stage should any oil spilled on it

A

cleanse

41
Q

REcap the immersion oil _______ securely, replace in drawer

A

container