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Parasitology > Parasitology Labs > Flashcards

Parasitology Labs Flashcards

0
Q

What is the generic name for s-125

A

Sulfadimethoxine

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1
Q

What is the generic name of pyran

A

Pyrantel pamoate

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2
Q

Between the modified knots technique and the difil test, which technique allows for differentiation Dirofilaria Immitis and Acanthochocheilonema reconditum microfilariae

A

Modified knotts technique

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3
Q

Why is it important to differentiate between D. Immitis and A. Reconditum ?

A

Because A. Reconditum doesn’t need to be treated

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4
Q

What are the morphological differences between both types of microfilariae

A

D. Immitis has a straight body with a tapered end

A. Reconditum has a curved body with a blunt head and a curved tail

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5
Q

Can a dog have heartworm and have a negative difil or knotts test ?

A

Yes if he has only adult male worms or the females could not have made microfilariae yet

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6
Q

Give two reasons why a dog might have heartworm and yet the rest Would come out negative

A

Due to the fact that it tests for females. If there is too little females or only males you might get a false negative

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7
Q

Give the kingdom, phylum and class of dipylidium caninum ova

A

Kingdom: Anamalia
Phylum: Platyhelminthes
Class: Cestoda

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8
Q

What is the the common name of dipylidium caninum ova

A

Flea tapeworm

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9
Q

Describe the dipylidium caninum ova

A

Oval with multiple circles inside. Transparent. No visible shell

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10
Q

Give the kingdom, phylum and class of echinococcus granulosis cyst

A

Kingdom: Anamalia
Phylum: Platyhelminthes
Class: Cestoda

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11
Q

What is the common name of echinococcus granulosus cyst

A

Dog worms

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12
Q

What are the general characteristics of echinococcus granulosus cysts

A

Rounded/ovalish cyst with small circle/oval circles inside

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13
Q

List the materials required for a fecal centrifugation technique

A

Slide, tongue depressor, paper cups, sample, sheathers solution

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14
Q

What is the density of sheathers solution

A

1200 units

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15
Q

Describe the fecal centrifugation technique

A

Put 1g of feces in a paper cup, put 1cc of sheathers solution in a cup. Make a soup. Put 8cc of sheathers solution. Mix it, in a second cup put 2 layers of gauze over the second cup and filter solution. Squeeze out extra liquid from gauze. Put all garbage in garbage cup

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16
Q

What type of centrifuge is ideal to perform fecal centrifugation

A

Swinging, the triac allows the coverslip to be placed on it.

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17
Q

Whats the difference between single and double centrifugation technique ? What is the purpose of doing the double centrifugation ?

A

Double centrifugation the 1st step is done with water instead of sheathers or znSO4. When you centrifuge it, te water is less dense than eggs so any sample will go to the bottom of the container. Centrifuge again, this time with sheathers and sample will raise to top

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18
Q

What are the materials required for a simple fecal flotation

A

Fecal cup, tongue depressor, zinc sulfate, cover slip, slide

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19
Q

Describe how to do a simple fecal flotation

A

Fill fecal tunnel, put zinc sulfate until divot is filled, turn until it forms a brown solution. Clip it down when the solution is nicely mixed. Full up to the top with zinc sulfate, make a miniscus, and place coverslip on top. Wait 10 mins and place it on slide

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20
Q

What are the disadvantages to using a direct smear

A

If you have a small amount of feces you can have a false negative.

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21
Q

What does a hydrometer measure

A

The specific gravity of a liquid

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22
Q

What does the Refractometer measure

A

The amount of dissolved Solutes in a solution

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23
Q

Which of the two should be used to measure specific gravity an check that your solutions are still good

A

The hydrometer

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24
Q

What is the kingdom, phylum and class of ancylostoma caninum ova

A

Kingdom: animalia
Phylum: Nematoda
Class: secementea

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25
Q

What are the general characteristics of ancylostoma caninum ova

A

Oval, transparent, granular appearance

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26
Q

What is the common name of ancylostoma caninum ova

A

Hookworm

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27
Q

What is the kingdom, phylum and class of trichinella spiralis encysted

A

Kingdom: animalia
Phylum: Nematoda
Class: adenophorea

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28
Q

What is the common name for trichinella spiralis encysted

A

Pork worm

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29
Q

What are the general characteristics of trichinella spiralis

A

Transparent worm in a muscle. Spiral shaped.

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30
Q

What is the kingdom, phylum and class of trichuris vulpis

A

Kingdom: animalia
Phylum: nemathelminthes
Class: Nematoda

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31
Q

What are the general characteristics of trichuris vulpis

A

Oval shaped, thick outer shell with bipolar plugs

32
Q

What is the common name for balis ascaris

A

Roundworm

33
Q

What is the kingdom, phylum, class of balis ascaris

A

Kingdom: animalia
Phylum: Nematoda
Class: secernentea

34
Q

What is the common name for w. Bankrofti

A

Roundworm

35
Q

What is the kingdom, phylum and class of w. Bankrofti

A

Kingdom: animalia
Phylum: Nematoda
Class: secernentea

36
Q

How is w. Bankrofti contracted

A

Mosquito

36
Q

What is the common name of shistosoma mansoni

A

Trematode

37
Q

What is the kingdom, phylum and class for shistosoma mansoni

A

Kingdom: animalia
Phylum: Platyhelminthes
Class: Trematoda

38
Q

What is the main carrier of shistosoma mansoni

A

Snails

39
Q

What is the main carrier of balis ascaris

A

Raccoon

40
Q

How do you differentiate between Taenia and dipylidium tapeworm

A

Taenia had 1 pore.

41
Q

How do you do the modified knotts test

A

1 ml blood+ 9ml formalin. Then centrifuge at 1500, 5 min. Pour off supernatant, stain and then examine sediment.

42
Q

Describe the fecal centrifugation technique

A

1g feces, 1cc sheathers, mix. Add 8cc sheathers. Mix. Filter one cup and pour solution in. Top it up to 13 cc. Put in centrifuge: 1500, 5 min. Top it up to make a miniscus and then put coverslip. Wait 5-10 mins

43
Q

How do you perform a skin scraping

A

Put mineral oil on crusty region, squeeze area, scrape area, put crust and material on slide with mineral oil and chop it up. Scrape till area turns red, put it on the slide and add coverslip.

44
Q

How do you perform the cellophane tape method

A

Take tape, sticky side down multiple times, mineral oil. Put tape on slide

45
Q

Describe the cellophane tape for pinworm identification

A

Tongue depressor, tape. Fold tape over tongue depressor with sticky side out and press it on the butt hole. Don’t use mineral oil on the slide.

46
Q

What is the class of balantidium coli trophozoites

A

Litostomatea

47
Q

What is the class of demodex follicularis

A

Arachnida

48
Q

What is the class of fasciola hepatica

A

Trematoda

49
Q

What is the common name of phthirus pubis adult

A

Crab louse

50
Q

What order of lice does phthirus pubis adult belong to and how can you tell simply by looking at it

A

Anoplura and it is due to the smaller head then it’s body

51
Q

What does the Anoplura phthirus pubis feed on

A

Blood

52
Q

Where does the phthirus pubis live on humans

A

In the pubic hair or any other course hair

53
Q

What is the scientific name of the head louse

A

Pediculus humanus capitis

54
Q

Where does the head louse live

A

On the hair of the head

55
Q

What is the baby form of a louse

A

A nit, or egg.

56
Q

How long does a nit take to hatch to nymph form

A

5-14 days

57
Q

What is the order of the cimex lectularis

A

Hemiptera

58
Q

What is the common name of cimex lectularis

A

Bed bug

59
Q

What is the order of melophagus ovinus

A

Diptera

60
Q

What is the common name of melophagus ovinus

A

Sheep ked

61
Q

What species are infected with sheep ked

A

Sheep and goats

62
Q

Where does the sheep ked live on the host

A

Deep into the Sheeps wool or the goats fleece

63
Q

What materials are needed for the baermann technique

A

Champagne glass with hollow tube, mesh cloth, gauze, rubber band

64
Q

What solution is use in the baermann technique

A

Water

65
Q

Describe the baermann technique

A

Put 5 g of feces in gauze, close the rubber band. Place mesh in glass to hold the gauze. Fill with room temp water so larva can swim out into the glass. Let it sit for 24hrs

66
Q

What is the purpose of conducting the baermann test

A

Larva of lungworm

67
Q

How is the sample for baermann test collected

A

Fresh sample from a hard surface

68
Q

What are three different possible solutions used for fecal shippin

A

SAF, formalin or 70% alcohol

69
Q

What is the ratio of the volume of fece to the volume of shipping solution

A

1/3 feces to solution

70
Q

What is the class for giardia trophozoites

A

Protozoa

71
Q

What are small segments of tapeworms called

A

Proglottids

72
Q

How is Taenia acquired

A

Ingestion of intermediate host

73
Q

If you are a solution with 1260 what are you

A

Sheathers

74
Q

What is the advantage to fecal centrifugation

A

More efficient at separating and its rapid.

75
Q

Why do we do the baermann technique

A

To find lungworm larva

76
Q

What is the scale to calibrate the microscope called

A

Stage micrometer

Parasitology (9 decks)

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