Parasitology Labs Flashcards
What is the generic name for s-125
Sulfadimethoxine
What is the generic name of pyran
Pyrantel pamoate
Between the modified knots technique and the difil test, which technique allows for differentiation Dirofilaria Immitis and Acanthochocheilonema reconditum microfilariae
Modified knotts technique
Why is it important to differentiate between D. Immitis and A. Reconditum ?
Because A. Reconditum doesn’t need to be treated
What are the morphological differences between both types of microfilariae
D. Immitis has a straight body with a tapered end
A. Reconditum has a curved body with a blunt head and a curved tail
Can a dog have heartworm and have a negative difil or knotts test ?
Yes if he has only adult male worms or the females could not have made microfilariae yet
Give two reasons why a dog might have heartworm and yet the rest Would come out negative
Due to the fact that it tests for females. If there is too little females or only males you might get a false negative
Give the kingdom, phylum and class of dipylidium caninum ova
Kingdom: Anamalia
Phylum: Platyhelminthes
Class: Cestoda
What is the the common name of dipylidium caninum ova
Flea tapeworm
Describe the dipylidium caninum ova
Oval with multiple circles inside. Transparent. No visible shell
Give the kingdom, phylum and class of echinococcus granulosis cyst
Kingdom: Anamalia
Phylum: Platyhelminthes
Class: Cestoda
What is the common name of echinococcus granulosus cyst
Dog worms
What are the general characteristics of echinococcus granulosus cysts
Rounded/ovalish cyst with small circle/oval circles inside
List the materials required for a fecal centrifugation technique
Slide, tongue depressor, paper cups, sample, sheathers solution
What is the density of sheathers solution
1200 units
Describe the fecal centrifugation technique
Put 1g of feces in a paper cup, put 1cc of sheathers solution in a cup. Make a soup. Put 8cc of sheathers solution. Mix it, in a second cup put 2 layers of gauze over the second cup and filter solution. Squeeze out extra liquid from gauze. Put all garbage in garbage cup
What type of centrifuge is ideal to perform fecal centrifugation
Swinging, the triac allows the coverslip to be placed on it.
Whats the difference between single and double centrifugation technique ? What is the purpose of doing the double centrifugation ?
Double centrifugation the 1st step is done with water instead of sheathers or znSO4. When you centrifuge it, te water is less dense than eggs so any sample will go to the bottom of the container. Centrifuge again, this time with sheathers and sample will raise to top
What are the materials required for a simple fecal flotation
Fecal cup, tongue depressor, zinc sulfate, cover slip, slide
Describe how to do a simple fecal flotation
Fill fecal tunnel, put zinc sulfate until divot is filled, turn until it forms a brown solution. Clip it down when the solution is nicely mixed. Full up to the top with zinc sulfate, make a miniscus, and place coverslip on top. Wait 10 mins and place it on slide
What are the disadvantages to using a direct smear
If you have a small amount of feces you can have a false negative.
What does a hydrometer measure
The specific gravity of a liquid
What does the Refractometer measure
The amount of dissolved Solutes in a solution
Which of the two should be used to measure specific gravity an check that your solutions are still good
The hydrometer
