Papillomavirus

Question 1

Pappilomavirus genome

Answer 1

Small, circular dsDNA

Question 2

Genotypes causing cutaneous warts

Answer 2

HPV 1,2

Question 3

Genotypes causing genital warts

Answer 3

HPV 6, 7

Question 4

Genotypes causing genital cancers

Answer 4

16, 18

Question 5

Epidermodisplasia verruciformis

Answer 5

HPV 5, 8

Question 6

Surface most easily infected

Answer 6

Mucosal surfaces

Question 7

HPV pathogenesis:
1)
2)
3)
4)

Answer 7

1) Infection establishes in basal layer of epithelium. Single episome in a cell.
2) Binds to host genome, divides when cell divides.
3) E6, E7 expressed, increased cell replication.
4) As cells approach skin surface, L1, L2 expressed. Capsids formed.

Question 8

E6, E7 effects

Answer 8

Bind p53, Rb. Increase cell division

Question 9

L1, L2 gene products

Answer 9

Capsid proteins

Question 10

Cervical cancer pathogenesis:
1)
2)
3)

Answer 10

1) Random integration into host genome
2) L1, L2 genes lost. If E2 gene lost, cells become malignant.
3) L1 vaccines no longer effective

Question 11

E2 role

Answer 11

Negatively regulate E6, E7 genes.

Question 12

Difficulty in mounting an immune response against HPV:
1)
2)
3)
4)

Answer 12

1) Virus only shed from skin surface.
2) No cell lysis.
3) No viral particles shed from cells.
4) No virus in the blood.

Question 13

Imiquimod

Answer 13

TLR7 ligand

Topically applied, stimulates immune response

Question 14

Gardasil contents

Answer 14

VLP of L1 gene product.

Vaccine taken at 0, 2 and 6 months.