Lab Identification of Pathogens Flashcards

1
Q

Bacillus cereus agar

A

Bacillus cereus selective agar (BCSA)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Bacillus cereus appearance on BCSA

A

Blue-green colonies surrounded by opaque precipitate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Baird Parker medium pathogens isolated

A

Staphylococcus aureus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Appearance of staph aureus on BP medium

A

Grey/black, shiny convex colonies.

Narrow white margin, zone of clearing.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Appearance of campylobacter on CAMP medium

A

Wet looking, slight metallic sheen.

Grow along streak lines

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Pathogens isolated with desoxycholate agar

A

Salmonella, shigella

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Agar used to culture shigella

A

Desoxycholate agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Agar used to culture salmonella

A

Desoxycholate agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Appearance of shigella on desoxycholate agar

A

Colourless-beige colonies, surrounded by a clear zone

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Appearance of salmonella on desoxycholate agar

A

Colourless-beige colonies, might have a central black spot, surrounded by a clear zone

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Uses of MacConkey agar

A

Selective, differential medium for isolating coliforms

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Coliforms

A

Escherichia, Klebisella, Enterobacter

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Distinguishing between lactose fermenters and non-fermenters on MAC agar

A

Lactose fermenters - pink

Lactose non-fermenters - colourless or very pale yellow colonies

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Selenite enrichment broth pathogens

A

Salmonella from food, stools, urine, water

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

BCSA selective elements

A

Polymixin B

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Baird Parker selective agents

A

Lithium, pyruvate, glycine, tellurite

17
Q

CAMP medium selective agents

A

Antibiotics, microaerophilic, 42 degrees C

18
Q

Desoxycholate agar selective agents

A

Sodium desoxycholate, sodium thiosulphate, ferric chloride, citrate

19
Q

MacConkey agar selective agents

A

Bile salts

20
Q

Selenite enrichment broth selective agents

A

Sodium selenite