Lab Identification of Pathogens Flashcards
Bacillus cereus agar
Bacillus cereus selective agar (BCSA)
Bacillus cereus appearance on BCSA
Blue-green colonies surrounded by opaque precipitate
Baird Parker medium pathogens isolated
Staphylococcus aureus
Appearance of staph aureus on BP medium
Grey/black, shiny convex colonies.
Narrow white margin, zone of clearing.
Appearance of campylobacter on CAMP medium
Wet looking, slight metallic sheen.
Grow along streak lines
Pathogens isolated with desoxycholate agar
Salmonella, shigella
Agar used to culture shigella
Desoxycholate agar
Agar used to culture salmonella
Desoxycholate agar
Appearance of shigella on desoxycholate agar
Colourless-beige colonies, surrounded by a clear zone
Appearance of salmonella on desoxycholate agar
Colourless-beige colonies, might have a central black spot, surrounded by a clear zone
Uses of MacConkey agar
Selective, differential medium for isolating coliforms
Coliforms
Escherichia, Klebisella, Enterobacter
Distinguishing between lactose fermenters and non-fermenters on MAC agar
Lactose fermenters - pink
Lactose non-fermenters - colourless or very pale yellow colonies
Selenite enrichment broth pathogens
Salmonella from food, stools, urine, water
BCSA selective elements
Polymixin B