Oxidative Phosphorylation Flashcards
Name the two mobile electron carriers.
Coenzyme Q(ubiquinone) and cytochrome C
Name the two substrate shuttle systems
Glyceral- phosphate shuttle
Malate aspartate shuttle
Which of the two shuttles uses both NAD+ and FAD+
Glyceral- phosphate shuttle
Function of Malate dehydrogenase in the malate aspartate shuttle.
Reduces oxaloacetate to malate in the presence of NADH+H
Oxaloacetate is converted to aspartate in the malate aspartate shuttle through what reaction
Transamination reaction
Which member of the electron transport chain is not a protein?
Coenzyme Q
Other name for complex I
NADH dehydrogenase
Which complex does not have enough energy to pump protons out?
Complex II(succinate dehydrogenase)
Which complex can react with oxygen directly?
Complex IV
Other name for complex III
Cytochrome bc1
Other name for complex IV
Cytochrome Oxidase
What’s the function of UCP I(thermogenin)
Production of heat( energy) in brown apidocytes
What kind of DNA produces protein needed for oxidative phosphorylation in the mitochondria?
mtDNA
Mutation of mitochondrial DNA is dash times faster than mutation of nuclear DNA
10x
Antimycin A inhibits electron flow through complex?
Complex III
Rotenone and amobarbital block electron flow through?
Complex I
Which complex contains copper atoms required for this reaction to occur?
Cytochrome c oxidase or complex Iv
Mention complex II inhibitors
Carboxin
Malate
Oxaloacetate
Complex 4 inhibitors
Cyanide
Sodium azide
CO
Hydrogen sulfide
Complex iii inhibitors
Antimycin A
Chelating
Stigmatellin
What’s the other name for chemiosmotic hypothesis
Mitchell hypothesis
What is Mitchell hypothesis?
Process that 3xplains how free energy generated by transport of electrons by ETC is used to produce ATP.
What does the Mitchell hypothesis process create?
pH gradient and electrical gradient (proton gradient)
Other name for complex v
ATP synthase
Name what ATP synthase contains and what they act as?
Fi and F0 domains
Act as rotational motor system
What is the Electron transport normally tightly coupled to
ATP Synthesis
Qheb ADP levels are high in the mitochondria, how is the rate of oxygen consumption?
Oxygen consumption arises as electrons flow down the chain
What happens to rate of Oxygen when ADP is phosphorylation?
Rate of oxygen utilisation falls
What inhibits the citric acid cycle and glycolysis
Build up of NADH AND FADH2
What does the Respiratory control mechanism ensure?
Ensures that electrons only flow down the chain when there is need for ATP synthesis
Defects of oxidative phosphorylation are as a result of…….?
Ulceration of mtDNA
2hat is the final acceptor of electrons in ETC?
O2 (oxygen)
How are electrons carried from ubiquinone to cytochrome c
Complex III
Prosthetic group associated with Fe-S is?
Complex II …succinate dehydrogenase
How dies FADH2 Transfer electrons?
Via fe-S in complex ii
Which mobile carrier is associated with the outer face of the inner membrane?
Cytochrome c
Electrons are transfered directly to CoQ minus passing through Complex I and II by
