Oxidative Phosphorylation

Question 1

Name the two mobile electron carriers.

Answer 1

Coenzyme Q(ubiquinone) and cytochrome C

Question 2

Name the two substrate shuttle systems

Answer 2

Glyceral- phosphate shuttle
Malate aspartate shuttle

Question 3

Which of the two shuttles uses both NAD+ and FAD+

Answer 3

Glyceral- phosphate shuttle

Question 4

Function of Malate dehydrogenase in the malate aspartate shuttle.

Answer 4

Reduces oxaloacetate to malate in the presence of NADH+H

Question 5

Oxaloacetate is converted to aspartate in the malate aspartate shuttle through what reaction

Answer 5

Transamination reaction

Question 6

Which member of the electron transport chain is not a protein?

Answer 6

Coenzyme Q

Question 7

Other name for complex I

Answer 7

NADH dehydrogenase

Question 8

Which complex does not have enough energy to pump protons out?

Answer 8

Complex II(succinate dehydrogenase)

Question 9

Which complex can react with oxygen directly?

Answer 9

Complex IV

Question 10

Other name for complex III

Answer 10

Cytochrome bc1

Question 11

Other name for complex IV

Answer 11

Cytochrome Oxidase

Question 12

What’s the function of UCP I(thermogenin)

Answer 12

Production of heat( energy) in brown apidocytes

Question 13

What kind of DNA produces protein needed for oxidative phosphorylation in the mitochondria?

Answer 13

mtDNA

Question 14

Mutation of mitochondrial DNA is dash times faster than mutation of nuclear DNA

Answer 14

10x

Question 15

Antimycin A inhibits electron flow through complex?

Answer 15

Complex III

Question 16

Rotenone and amobarbital block electron flow through?

Answer 16

Complex I

Question 17

Which complex contains copper atoms required for this reaction to occur?

Answer 17

Cytochrome c oxidase or complex Iv

Question 18

Mention complex II inhibitors

Answer 18

Carboxin
Malate
Oxaloacetate

Question 19

Complex 4 inhibitors

Answer 19

Cyanide
Sodium azide
CO
Hydrogen sulfide

Question 20

Complex iii inhibitors

Answer 20

Antimycin A
Chelating
Stigmatellin

Question 21

What’s the other name for chemiosmotic hypothesis

Answer 21

Mitchell hypothesis

Question 22

What is Mitchell hypothesis?

Answer 22

Process that 3xplains how free energy generated by transport of electrons by ETC is used to produce ATP.

Question 23

What does the Mitchell hypothesis process create?

Answer 23

pH gradient and electrical gradient (proton gradient)

Question 24

Other name for complex v

Answer 24

ATP synthase

Question 25

Name what ATP synthase contains and what they act as?

Answer 25

Fi and F0 domains

Act as rotational motor system

Question 26

What is the Electron transport normally tightly coupled to

Answer 26

ATP Synthesis

Question 27

Qheb ADP levels are high in the mitochondria, how is the rate of oxygen consumption?

Answer 27

Oxygen consumption arises as electrons flow down the chain

Question 28

What happens to rate of Oxygen when ADP is phosphorylation?

Answer 28

Rate of oxygen utilisation falls

Question 29

What inhibits the citric acid cycle and glycolysis

Answer 29

Build up of NADH AND FADH2

Question 30

What does the Respiratory control mechanism ensure?

Answer 30

Ensures that electrons only flow down the chain when there is need for ATP synthesis

Question 31

Defects of oxidative phosphorylation are as a result of…….?

Answer 31

Ulceration of mtDNA

Question 32

2hat is the final acceptor of electrons in ETC?

Answer 32

O2 (oxygen)

Question 33

How are electrons carried from ubiquinone to cytochrome c

Answer 33

Complex III

Question 34

Prosthetic group associated with Fe-S is?

Answer 34

Complex II …succinate dehydrogenase

Question 35

How dies FADH2 Transfer electrons?

Answer 35

Via fe-S in complex ii

Question 36

Which mobile carrier is associated with the outer face of the inner membrane?

Answer 36

Cytochrome c

Question 37

Electrons are transfered directly to CoQ minus passing through Complex I and II by