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Oxidase Positive GNR + Misc GNR Flashcards

1
Q

General Characteristics

A

Growth on BAP and MAC
Curved or straight GNR
Oxidase Positive
Ferments glucose
Many associated with GI or wound infections

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2
Q

General Growth Vibrio species

A

Growth on BAP and MAC
TCBS Agar
Curved GNR
Halophilic
Ferments Glucose
KIA = AK/A or A/A
Oxidase = Positive
Catalase = Positive
Indole = Positive
Nitrate = Positive
Gel = Positive
Inositol = Negative
String Test
Motile

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3
Q

Vibrio species LAO

A

+/-/+

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4
Q

Vibrio cholera Disease Info

A

Serious GI tract
Fatal Diarrhea and vomiting
Rice-water stools - 20L/day loss of H2O

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5
Q

Vibrio cholera Reactions

A

Lactose = Negative
Sucrose = Positive (TCBS yellow)
VP = Positive
Salicin = Negative
ONPG = Positive
0-1% NaCl
Vibriostatic sensitive

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6
Q

Vibrio parahaemolyticus Disease

A

Second Most Common GI
Mostly US
Contaminated seafood
Watery diarrhea and wound infections
Incubation 4-30 hrs

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7
Q

Vibrio parahaemolyticus Reactions

A

Lactose = Negative
Sucrose = Negative (TCBS blue)
VP = Negative
ONPG = Negative
Salicin = Negative
3-8% NaCl growth
Vibriostatic sensitive only at 150 ug

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8
Q

Vibrio vulnificus Disease

A

Seond most serious Infection
North America and Hawaii
Septicemias and wound infections
40-60% mortality in hours

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9
Q

Vibrio vulnificus Reactions

A

Lactose = Positive
Sucrose = Negative (TCBS blue)
VP = Negative
ONPG = Positive
Salicin = Positive
3-6% NaCl growth
Vibriostatic sensitive

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10
Q

Vibrio alginolyticus Disease

A

Wound and ear infection

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11
Q

Vibrio alginolyticus Reactions

A

Lactose = Negative
Sucrose = Positive (TCBS yellow)
VP = Positive
ONPG = Positive
Salicin = Negative
3-10% NaCl
Vibriostatic sensitive

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12
Q

Aeromonas hydrophila Disease

A

Septicemia and Wound infections
Intestinal diarrhea
Gastroenteritis

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13
Q

Aeromonas hydrophila Morphology

A

Beta hemolysis
MAC: LF or NLF (Variable)
CIN = Pink CFUs
KIA and TSI = A/A or AK/A
GS = straight GNR

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14
Q

Aeromons hydrophila Reactions

A

Oxidase = Positive
Catalase = Positive
Indole = Positive
VP = Positive
Inositol = Negative
Gel = Positive
Esculin = Positive
Motile
Ferments Glucose
Vibriostatic Resistant

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15
Q

Aeromonas hydrophila LAO

A

+/+/-

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16
Q

Plesiomonas shigelloides Disease

A

Gastroenteritis
Wounds
Bacteremia
Meningitis

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17
Q

Plesiomonas shigelloides Morphology

A

Shiny, Opaque
MAC: NLF
GS = straight GNR

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18
Q

Plesiomonas shigelloides Reactions

A

Oxidase = Positive
VP = Negative
Gel = Negative
VP = Negative
Inositol = Positive
Ferments Lactose
Vibriostatic sensitive
6.5% NaCl = NG

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19
Q

Plesiomonas shigelloides LAO

A

+/+/+

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20
Q

Campylobacter species Disease

A

Associated with poultry and cattle
Causes gastroenteritis
Campylobacter jejuni
Campylobacter coli
Campylobacter fetus

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21
Q

Campylobacter coli Morphology

A

CVA media: Colonies are spreading along inoculation line
Growth at 37-42 C
5-10% O2, 10% CO2, rest N2

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22
Q

Campylobacter coli Reactions

A

Hippurate = Negative
Naldixic = Sensitive

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23
Q

Campylobacter fetus Morphology

A

CVA media
Growth at 25-35 C
5-10% O2, 10% CO2, rest N2

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24
Q

Campylobacter fetus Reactions

A

Hippurate = Negative
Naldixic = Resistant

25
Campylobacter jejuni Morphology
CVA media = CFU are spreading along inoculation line Growth at 37-42 C 5-10% O2, 10% CO2, rest N2
26
Campylobacter jejuni Reactions
Hippurate = Positive Naldixic = Sensitive
27
Helicobacter DIsease
Chronic gastristis Peptic Ulcer
28
Helicobacter Testing
GS = Curved CNR C test using marked C13/14 Oxidase = Positive Catalase = Negative Rapid Urea = Positive ** Not Grown in Lab **
29
Pasteurella multocida Disease
Zoonotic Inflamed lesion at bite
30
Pasteurella multocida Morphology
BAP: Gry smooth, moist Brownish/green at 48 hours GS: GNCB with bipolar staining
31
Pasteurella multocida Reactions
Oxidase = Positive Catalase = Positive Indole = Positive Urea = Negative H2S = Positive Nitrate = Positive Ferments: Glucose, Mannitol, Galactose, Dulcitol, and Sorbitol Non-motile
32
Pasteurella multocida LAO
-/-/+
33
Francisella tularensis Disease Info
Humans are accidental host Rabbit fever Category A Bioagent (5-15% mortality) 45-80% Ulcer glandular disease Tularemic pneumonia (most serious) Typhoidal tularemia
34
Francisella tularensis Morphology
Growth on BCYE, CHOC, TM Agar CFUs need 48-72 hours to grow Coloines = gry-white, raised, smooth (mucoid)
35
Francisella tularensis Reactions
Oxidase = Positive Catalase = Weak Positive Beta-lactamase = Weak Positive
36
Brucellosis
Caused by Brucella Acute Infection Subchronic infection Chronic Infection
37
Brucellosis Acute Infection
Irregular fever and chills Malaise Headache Weight Loss Weakness Fatigue Anorexia Nausea Vomiting Diarrhea
38
Brucellosis Subchronic Infection
Undulant fever Normal temperature in am followed by high temperature in pm Arthritis Inflammation - within 1 yr of exposure
39
Brucellosis Chronic Infection
Depression Arthritis and Chronic fatigue syndrome (year after exposure)
40
Bordetella pertusis Disease
Whooping cough Sneezing Running nose Slight fever Cough in spasms
41
Bordetella pertusis Reactions
Bordet-Gengou or BCYA with 10% horse blood and 40 mg/dL Catalase = Positive (variable) Oxidase = Positive Nitrate = Negative Urease = Negative Non-motile
42
Bordetella parapertusis Disease
Mild Whooping Cough
43
Bordetella parapertusis Reactions
Growth on BAP, NG on MAC Catalase = Positive Oxidase = Negative Nitrate = Negative Urea = Positive (24 hours) Non-motile
44
Bordetella bronchiseptica Disease
Kennel Cough Can cause infections in immunocompromised
45
Bordetella bronchiseptica Reactions
Catalase = Positive Oxidase = Positive Nitrate = Positive Urea = Positive (within 4 hrs) Motile
46
Legionella pneumophilia Info
Aquatic ecosystems Hot Springs and Lakes Airborne spread
47
Legionella pneumophilia Disease
Causes pneumonia and dissemination to other organs 8-18K hospitalized yearly 15-30% mortality rate Pontiac Fever: Milder form of disease, not associated with mortality
48
Legionella pneumophilia Morphology
BCYE with cysteine at 35-37 C ** Hold for two weeks ** GS = Faint Staining, Thin GNR
49
Brucella zoonotic infections
B. abortus = cattle B. suis = swine B. melitensis = goats and sheep B. canis = dogs (especially beagles)
50
Bartonella henselae Disease
Cat Scratch Fever Humans are infected with scratch Symptoms begin 3-14 days later Non-painful bump or blister at site Painful swollen lymph nearest scratch (up to 3-4 mos to resolve) Exhaustion Headache Fever
51
Bartonella henselae Reactions
GS = Slightly curved GNR Oxidase = Negative Slow growng Serology is more accurate Draw acute and convalescent sera 10 days to 2 weeks apart Look for 4 fold increase PCR
52
Leptospira interrogans Disease
Primarily in children - causes Leptospirosis Children contact from cat urine Spreads via blood to CNS and kidneys - 10% fatality
53
Leptospira interrogans Media
Fletchers or Stuart
54
Borrelia recurrentis Disease
Human relapsing fever - identified in hematology smear Symptoms appear in 2-15 days Fever Headache and myalgia of 4-10 days duration
55
Borrelia recurrentis Transmitted
Bite of tick or louse Borrelia hermsii
56
Borrelia burgdorferi Disease
Lyme's disease Stage 1: Bullseye lesion at site of tick bite Stage 2: Arthritis and neurological complications Stage 3: Chronic arthritis, memory loss, and neurological symptoms
57
Borrelia burgdorferi Identified
Serological testing PCR
58
Treponema pallidium Disease
Syphilis Stage 1: Painless ulcer or chanre (1-3 mos) Stage 2: Rash in mucosa and skin (6 wks to 6 mos) Stage 3: 30% develop heart and CNS involvement 2-20 yrs later
59
Treponema pallidium Identification
Cannot be cultured Identified using dark-field microscopy Serology - RPR or VDRL (antibody testing) FTA-Abs = Confirmatory test

Microbiology (9 decks)

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