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Microbiology > Oxidase Positive GNR + Misc GNR > Flashcards

Oxidase Positive GNR + Misc GNR Flashcards

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1
Q

General Characteristics

A

Growth on BAP and MAC
Curved or straight GNR
Oxidase Positive
Ferments glucose
Many associated with GI or wound infections

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2
Q

General Growth Vibrio species

A

Growth on BAP and MAC
TCBS Agar
Curved GNR
Halophilic
Ferments Glucose
KIA = AK/A or A/A
Oxidase = Positive
Catalase = Positive
Indole = Positive
Nitrate = Positive
Gel = Positive
Inositol = Negative
String Test
Motile

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3
Q

Vibrio species LAO

A

+/-/+

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4
Q

Vibrio cholera Disease Info

A

Serious GI tract
Fatal Diarrhea and vomiting
Rice-water stools - 20L/day loss of H2O

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5
Q

Vibrio cholera Reactions

A

Lactose = Negative
Sucrose = Positive (TCBS yellow)
VP = Positive
Salicin = Negative
ONPG = Positive
0-1% NaCl
Vibriostatic sensitive

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6
Q

Vibrio parahaemolyticus Disease

A

Second Most Common GI
Mostly US
Contaminated seafood
Watery diarrhea and wound infections
Incubation 4-30 hrs

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7
Q

Vibrio parahaemolyticus Reactions

A

Lactose = Negative
Sucrose = Negative (TCBS blue)
VP = Negative
ONPG = Negative
Salicin = Negative
3-8% NaCl growth
Vibriostatic sensitive only at 150 ug

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8
Q

Vibrio vulnificus Disease

A

Seond most serious Infection
North America and Hawaii
Septicemias and wound infections
40-60% mortality in hours

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9
Q

Vibrio vulnificus Reactions

A

Lactose = Positive
Sucrose = Negative (TCBS blue)
VP = Negative
ONPG = Positive
Salicin = Positive
3-6% NaCl growth
Vibriostatic sensitive

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10
Q

Vibrio alginolyticus Disease

A

Wound and ear infection

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11
Q

Vibrio alginolyticus Reactions

A

Lactose = Negative
Sucrose = Positive (TCBS yellow)
VP = Positive
ONPG = Positive
Salicin = Negative
3-10% NaCl
Vibriostatic sensitive

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12
Q

Aeromonas hydrophila Disease

A

Septicemia and Wound infections
Intestinal diarrhea
Gastroenteritis

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13
Q

Aeromonas hydrophila Morphology

A

Beta hemolysis
MAC: LF or NLF (Variable)
CIN = Pink CFUs
KIA and TSI = A/A or AK/A
GS = straight GNR

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14
Q

Aeromons hydrophila Reactions

A

Oxidase = Positive
Catalase = Positive
Indole = Positive
VP = Positive
Inositol = Negative
Gel = Positive
Esculin = Positive
Motile
Ferments Glucose
Vibriostatic Resistant

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15
Q

Aeromonas hydrophila LAO

A

+/+/-

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16
Q

Plesiomonas shigelloides Disease

A

Gastroenteritis
Wounds
Bacteremia
Meningitis

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17
Q

Plesiomonas shigelloides Morphology

A

Shiny, Opaque
MAC: NLF
GS = straight GNR

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18
Q

Plesiomonas shigelloides Reactions

A

Oxidase = Positive
VP = Negative
Gel = Negative
VP = Negative
Inositol = Positive
Ferments Lactose
Vibriostatic sensitive
6.5% NaCl = NG

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19
Q

Plesiomonas shigelloides LAO

A

+/+/+

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20
Q

Campylobacter species Disease

A

Associated with poultry and cattle
Causes gastroenteritis
Campylobacter jejuni
Campylobacter coli
Campylobacter fetus

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21
Q

Campylobacter coli Morphology

A

CVA media: Colonies are spreading along inoculation line
Growth at 37-42 C
5-10% O2, 10% CO2, rest N2

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22
Q

Campylobacter coli Reactions

A

Hippurate = Negative
Naldixic = Sensitive

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23
Q

Campylobacter fetus Morphology

A

CVA media
Growth at 25-35 C
5-10% O2, 10% CO2, rest N2

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24
Q

Campylobacter fetus Reactions

A

Hippurate = Negative
Naldixic = Resistant

25
Q

Campylobacter jejuni Morphology

A

CVA media = CFU are spreading along inoculation line
Growth at 37-42 C
5-10% O2, 10% CO2, rest N2

26
Q

Campylobacter jejuni Reactions

A

Hippurate = Positive
Naldixic = Sensitive

27
Q

Helicobacter DIsease

A

Chronic gastristis
Peptic Ulcer

28
Q

Helicobacter Testing

A

GS = Curved CNR
C test using marked C13/14
Oxidase = Positive
Catalase = Negative
Rapid Urea = Positive
** Not Grown in Lab **

29
Q

Pasteurella multocida Disease

A

Zoonotic
Inflamed lesion at bite

30
Q

Pasteurella multocida Morphology

A

BAP: Gry smooth, moist
Brownish/green at 48 hours
GS: GNCB with bipolar staining

31
Q

Pasteurella multocida Reactions

A

Oxidase = Positive
Catalase = Positive
Indole = Positive
Urea = Negative
H2S = Positive
Nitrate = Positive
Ferments: Glucose, Mannitol, Galactose, Dulcitol, and Sorbitol
Non-motile

32
Q

Pasteurella multocida LAO

A

-/-/+

33
Q

Francisella tularensis Disease Info

A

Humans are accidental host
Rabbit fever
Category A Bioagent (5-15% mortality)
45-80% Ulcer glandular disease
Tularemic pneumonia (most serious)
Typhoidal tularemia

34
Q

Francisella tularensis Morphology

A

Growth on BCYE, CHOC, TM Agar
CFUs need 48-72 hours to grow
Coloines = gry-white, raised, smooth (mucoid)

35
Q

Francisella tularensis Reactions

A

Oxidase = Positive
Catalase = Weak Positive
Beta-lactamase = Weak Positive

36
Q

Brucellosis

A

Caused by Brucella
Acute Infection
Subchronic infection
Chronic Infection

37
Q

Brucellosis Acute Infection

A

Irregular fever and chills
Malaise
Headache
Weight Loss
Weakness
Fatigue
Anorexia
Nausea
Vomiting
Diarrhea

38
Q

Brucellosis Subchronic Infection

A

Undulant fever
Normal temperature in am followed by high temperature in pm
Arthritis
Inflammation - within 1 yr of exposure

39
Q

Brucellosis Chronic Infection

A

Depression
Arthritis and Chronic fatigue syndrome (year after exposure)

40
Q

Bordetella pertusis Disease

A

Whooping cough
Sneezing
Running nose
Slight fever
Cough in spasms

41
Q

Bordetella pertusis Reactions

A

Bordet-Gengou or BCYA with 10% horse blood and 40 mg/dL
Catalase = Positive (variable)
Oxidase = Positive
Nitrate = Negative
Urease = Negative
Non-motile

42
Q

Bordetella parapertusis Disease

A

Mild Whooping Cough

43
Q

Bordetella parapertusis Reactions

A

Growth on BAP, NG on MAC
Catalase = Positive
Oxidase = Negative
Nitrate = Negative
Urea = Positive (24 hours)
Non-motile

44
Q

Bordetella bronchiseptica Disease

A

Kennel Cough
Can cause infections in immunocompromised

45
Q

Bordetella bronchiseptica Reactions

A

Catalase = Positive
Oxidase = Positive
Nitrate = Positive
Urea = Positive (within 4 hrs)
Motile

46
Q

Legionella pneumophilia Info

A

Aquatic ecosystems
Hot Springs and Lakes
Airborne spread

47
Q

Legionella pneumophilia Disease

A

Causes pneumonia and dissemination to other organs
8-18K hospitalized yearly
15-30% mortality rate

Pontiac Fever: Milder form of disease, not associated with mortality

48
Q

Legionella pneumophilia Morphology

A

BCYE with cysteine at 35-37 C
** Hold for two weeks **
GS = Faint Staining, Thin GNR

49
Q

Brucella zoonotic infections

A

B. abortus = cattle
B. suis = swine
B. melitensis = goats and sheep
B. canis = dogs (especially beagles)

50
Q

Bartonella henselae Disease

A

Cat Scratch Fever
Humans are infected with scratch
Symptoms begin 3-14 days later
Non-painful bump or blister at site
Painful swollen lymph nearest scratch (up to 3-4 mos to resolve)
Exhaustion
Headache
Fever

51
Q

Bartonella henselae Reactions

A

GS = Slightly curved GNR
Oxidase = Negative
Slow growng

Serology is more accurate
Draw acute and convalescent sera 10 days to 2 weeks apart
Look for 4 fold increase
PCR

52
Q

Leptospira interrogans Disease

A

Primarily in children - causes Leptospirosis
Children contact from cat urine
Spreads via blood to CNS and kidneys - 10% fatality

53
Q

Leptospira interrogans Media

A

Fletchers or Stuart

54
Q

Borrelia recurrentis Disease

A

Human relapsing fever - identified in hematology smear
Symptoms appear in 2-15 days
Fever
Headache and myalgia of 4-10 days duration

55
Q

Borrelia recurrentis Transmitted

A

Bite of tick or louse
Borrelia hermsii

56
Q

Borrelia burgdorferi Disease

A

Lyme’s disease
Stage 1: Bullseye lesion at site of tick bite
Stage 2: Arthritis and neurological complications
Stage 3: Chronic arthritis, memory loss, and neurological symptoms

57
Q

Borrelia burgdorferi Identified

A

Serological testing
PCR

58
Q

Treponema pallidium Disease

A

Syphilis
Stage 1: Painless ulcer or chanre (1-3 mos)
Stage 2: Rash in mucosa and skin (6 wks to 6 mos)
Stage 3: 30% develop heart and CNS involvement 2-20 yrs later

59
Q

Treponema pallidium Identification

A

Cannot be cultured
Identified using dark-field microscopy
Serology - RPR or VDRL (antibody testing)
FTA-Abs = Confirmatory test

Microbiology (9 decks)

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