Fastidious GNRs Flashcards

1
Q

Haemophilus species General Info

A

GS = GNCB
Require special growth media containing X, V, and XV factor
Needs increased CO2 content
Non-motile

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2
Q

Haemophilus influenzae Growth Info

A

Growth only on Choc
No Hemolysis on Horse Blood
Requires X and V Factor
ALA = Negative (Pathogenic)
Beta Lactamase Test Required

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3
Q

Haemophilus influenzae Disease

A

Meningitis - Children Under 6
Epiglottis
Otitis media
Acute Sinusitis
URT infection and chronic bronchitis
Pneumonia
Bacteremia
Septicemia
Endocarditis
Conjuctivities

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4
Q

Haemophilus influenzae biotype agypticus Disease

A

Conjunctivities (Pink Eye)

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5
Q

Haemophilus parainfluenzae/paraphrophilus Growth

A

Growth on Choc
No Hemolysis on Horse Blood
Requires V Factor
ALA = Positive (non-pathogenic)

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6
Q

Haemophilus hemolyticus Growth

A

Growth on Choc
Beta hemolysis on Horse Blood
Requires X and V Factor
ALA = Negative (Pathogenic)

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7
Q

Haemophilus parahaemolyticus Growth

A

Growth on Choc
Beta Hemolysis on Horse Blood
Requires V Factor
ALA = Positive (non-pathogenic)

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8
Q

Haemophilus ducreyi Growth

A

Growth on BAP and Choc (slow growing)
GS = School of Fish
Soft Chancroid (papules and ulcers) on genitals and lymph nodes
More common in tropical areas

** Sexually Transmitted **

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9
Q

Capnocytophaga carimorsus Disease

A

Endocarditis
Blood Cultures

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10
Q

Capnocytophaga carimorsus Identification

A

GS: GNR or GNCB
Catalase = Positive
Oxidase = Positive (Variable)
G/M/L/S: +/+/+/-
Dog/cat bite pathogen

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11
Q
A
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11
Q

Capnocytophaga carimorsus G/M/L/S

A

+/+/+/-

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12
Q

Aggregatabacter actinomycetemcomitans Disease

A

Endocarditis
Blood Culture

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13
Q

Aggregatabacter actinomycetemcomitans Reactions

A

GS: GNR or GNCB
Catalase = Positive
Oxidase = Varibale
Indole = Negative
MAC: NG

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14
Q

Aggregatabacter actinomycetemcomitans G/M/L/S (Glucose/Maltose/Lactose/Sucrose)

A

+/+/-/-

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15
Q

Aggregatabacter actinomycetemcomitans LAO

A

-/-/-

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16
Q

Capnocytophaga species Disease

A

Endocarditis
Blood Culture
Human Oral Flora

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17
Q

Capnocytophaga species Reactions

A

Gliding motility
Yellow/orange pigment - colony sweats
Catalase = Negative
Oxidase = Negative

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18
Q

Capnocytophaga species G/M/L/S

A

+/+/V/V

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19
Q

Capnocytophaga species LAO

A

-/-/-

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20
Q

Kingella denitrificans Disease

A

Endocarditis
Blood Culture

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21
Q

Kingella denitrificans Reactions

A

Catalase = Negative
Oxidase = Positive or Variable
Nitrate = Positive
Urea = Negative
Indole = Negative
Esculin = Negative
Gelatin = Negative
Citrate = Negative
Non-motile

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22
Q

Kingella denitricans G/M/L/S

23
Q

Kingella kingae Disease

A

Endocarditis
Blood Culture

24
Kingella kingae Reactions
Catalase = Negative Oxidase = Positive or variable Nitrate = Negative Non-motile
25
Kingella kingae Morphology
2 Colony types Pinpoint at 48 hours 1) Soft Beta 2) Spreading, corroding
26
Kingella kingae G/M/L/S
+/+/-/-
27
Aggregatibacter aphrophilus Disease
Endocarditis Blood Culture
28
Aggregatibacter aphrophilus Morphology
Colonies have yellow tinge Associated with tongue piercing Factory Requirement: V variable
29
Aggregatibacter aphrophilus Reactions
Catalase = Negative Oxidase = Positive or variable ALA = May be weak positive
30
Aggregatibacter aphrophilus G/M/L/S
+/+/+/+
31
Cardiobacterium hominis Disease
Endocarditis Blood Culture
32
Cardiobacterium hominis Morphology
GS: Teardrops, dumbells BAP & Choc: ppt growth at 48 hrs, may pit agar
33
Cardiobacterium hominis Reactions
Catalase = Negative Oxidase = Positive or varibale Indole = Positive
34
Cardiobacterium homins G/M/L/S
+/V/-/+
35
Eikinella corrodens Disease
Blood Culture Closed fist injuries Endocarditis Bacteremia Respiratory Tract Infections Head and Neck Infections Periodontal Infections
36
Eikinella corrodens Reactions
GS: GNR - slender Smells like bleach Catalase = Negative Oxidase = Positive or Variable
37
Eikinella corrodens G/M/L/S
-/-/-/-
38
Eikinella corrodens LAO
+/-/+
39
Neisseria species General Characteristics
Aerobic GS: GNDC Oxidase = Positive Catalase = Positive - most species Non-motile Acid from oxidation of carbohydrates (not fermentation)
40
Neisseria gonorrhoeae Info
Sexually transmitted disease Growth best on Choc Beta Lactamase
41
Neisseria gonorrhoeae G/M/L/S
+/-/-/-
42
Neisseria meningitidis Info
Humans only natural host Growth on Choc best Oxidase = Positive
43
Neisseria meningititids Disease
Meningitis Septicemia Meningoencephalitis Pneumonia Arthritis Urethritis
44
Neisseria meningitidis G/M/L/S
+/+/-/-
45
Neisseria lactamica Info
Growth best on Choc
46
Neisseria lactamica G/M/L/S
+/+/+/-
47
Neisseria sicca/mucosa Info
Growth best on Choc
48
Neisseria sicca/mucosa G/M/L/S
+/+/-/+
49
Moraxella catarrhalis Info
Growth best on Choc Oxidase = Positive DNAse = Positive Butyrate esterase = Positive Catarrhalis disk = Positive Beta Lactamase
50
Moraxella catarrhalis G/M/L/S
-/-/-/-
51
Moraxella catarrhalis Disease
Children = otitis media & sinusitis Adult = bronchitis, pneumonia, bacteremia, endocarditis, meningitis, eye, wounds, and urogenital infections
52
Niesseria flavescens and polysaccharea
Rarely associated with infection
53
N. sicca, N. Flava, N. subflava, N. perflava, N. mucosa
Rarely causes septicemia, Endocarditis, meningitis, pneumonia, septic arthritis
54
Niesseria elongata Disease
Endocarditis Septicemia Osteomyelitis