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Microbiology > Enteric and NLF GNRs > Flashcards

Enteric and NLF GNRs Flashcards

1
Q

Citrobacter freundii

A

Citrate = Positive
Lysine = Negative
ONPG = Positive
KIA = A/AK/A

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2
Q

Citrobacter freundii Lactose Fermentation

A

Mostly NLF
Some subspecies may be LF

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3
Q

Citrobacter freundii MIO

A

+/-/-

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4
Q

Serratia marcescens

A

ONPG = Positive
DNAse = Positive
Gel = Positive
Ara = Negative
KIA = AK/A

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5
Q

Serratia marcescens Morphology

A

BAP colonies = Pink to Red
MAC = Dark Pink to Red
NLF

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6
Q

Serratia marcescens MIO

A

+/-/+

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7
Q

Yersinia pestis

A

NLF
ONPG = Positive
Urea = Negative
Ornithine = Negative
KIA = AK/A

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8
Q

Yersinia pestis MIO

A

-/-/-

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9
Q

Pseudomonas aeruginosa

A

MOST COMMON NLF
Oxidase = Positive
Catalase = Positive
Non-motile
Growth at 42 C
Nitrate = Positive
Gel = Positive
OF/O = Positive
OF/F = Negative

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10
Q

Pseudomonas aeruginosa Morphology

A

Beta Hemolytic
Pyocyanin
Grape or Tortilla Scent
NLF

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11
Q

Pseudomonas aeruginosa LAO

A

-/+/-

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12
Q

Pseudomonas stutzeri Morphology

A

Yellow/Tan
Wrinkled
Leathery
NLF

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13
Q

Pseudomonas stutzeri

A

Oxidase = Positive
Motile
Growth at 42 C
Oxidizes Glucose, Lactose, Maltose, and Fructose
Reduces Nitrate to N2

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14
Q

Pseudomonas stutzeri LAO

A

-/-/-

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15
Q

Acinetobacter species

A

Gram Stain = GNCB
Acinetobacter baumannii
Acinetobacter lwoffi

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16
Q

Acinetobacter baumannii Morphology

A

NLF
Mauve Appearance on MAC

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17
Q

Acinetobacter baumannii

A

Oxidase = Negative
Catalase = Negative
Nonmotile
Oxidizes Glucose and Xylose

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18
Q

Acinetobacter baumannii LAO

A

-/-/-

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19
Q

Acinetobacter lwoffi

A

Oxidase = Negative
Nitrate = Negative
Nonmotile

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20
Q

Acinetobacter lwoffi LAO

A

-/-/-

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21
Q

PDA + Organisms

A

Proteus species
Morganella morganii
Providencia species

Parts Per Million Mnemonic Device for Proteus, Providencia, Morganella

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22
Q

Proteus species

A

Proteus mirabilis
Proteus vulgaris

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23
Q

Proteus mirabilis

A

PDA = Positive
H2S = Positive
KIA = AK/A

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24
Q

Proteus mirabilis MIO

A

+/-/+

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25
Proteus vulgaris
PDA = Positive H2S = Positive KIA = AK/A
26
Proteus vulgaris MIO
+/+/- ** Vulgaris is Icky**
27
Morganella morganii
PDA = Positive H2S = Negative KIA = AK/A
28
Morganella morganii MIO
+/+/+
29
Providencia species
Providencia rettgeri Providencia stuartii
30
Providencia rettgeri
PDA = Positive H2S = Negative Urea = Positive Mannitol = Positive KIA = AK/A
31
Providencia rettgeri MIO
+/+/-
32
Providencia stuartii
PDA = Positive H2S = Negative Urea = Negative (30% are Positive) Mannitol = Negative KIA = AK/A
33
Providencia stuartii MIO
+/+/-
34
H2S Positive Organisms
Edwardsiella tarda Citrobacter freundii Salmonella species ** SEC **
35
Edwardsiella tarda
PDA = Negative H2S = Positive Citrate = Negative ONPG = Negative KIA = AK/A
36
Edwardsiella tarda MIO
+/+/+
37
Salmonella species
PDA = Negative H2S = Positive Citrate = Negative Lsyine = Positive KIA = AK/A Serotype
38
Salmonella species MIO
+/-/+
39
VP (Voges-Proskauer) Positive Organisms
Klebsiella species Enterobacter species Serratia species ** KES = Kryptonite Enrages Superman **
40
Klebsiella species
Klebsiella pneumoniae Klebsiella oxytoca Klebsiella (Enterobacter) aerogenes
41
Klebsiella pneumoniae
PDA = Negative H2S = Negative VP = Positive KIA = A/A
42
Klebsiella pneumoniae MIO
-/-/-
43
Klebsiella oxytoca
PDA = Negative H2S = Negative VP = Positive KIA = A/A
44
Klebsiella oxytoca MIO
-/+/- ** Y is Ideal for Indole **
45
Enterobacter species
Enterobacter (Klebsiella aerogenes) Enterobacter cloacae
46
Enterobacter (Klebsiella) aerogenes
PDA = Negative H2S = Negative VP = Positive Lysine = Positive Arginine = Negative KIA = A/A ** Better if A had been positive to correspond with aerogenes **
47
Enterobacter (Klebsiella) aerogenes MIO
+/-/+
48
Enterobacter cloacae
PDA = Negative H2S = Negative VP = Positive Lysine = Negative Arginine = Positive
49
Enterobacter cloacae MIO
+/-/+
50
Non-Motile Enteric Organisms
Shigella species Klebsiella species Yersinia species ** SKY **
51
Shigella species Serotyping
A, B, C, D Dirty = Shigella dysenteriae (A) Fingers = Shigella flexneri (B) Brings = Shigella boydii (C) Shigella = Shigella sonnei (D)
52
Shigella species A, B, C
very nonreactive ONPG = Negative Ornithine = Negative KIA = AK/A
53
Shigella species D
ONPG = Positive Ornithine = Positive KIA = AK/A
54
Yersinia enterocolitica
NLF Very nonreactive ONPG = Positive Motile at 25 C Ornithine = Positive Urea = Positive KIA = AK/A
55
Yersinia enterocolitica MIO
-/-/-
56
Escherichia coli
Mostly LF (Some are NLF) VP = Negative MR = Positive Lysine = Positive Arginine = Variable KIA = A/A H2S = Negative
57
Escherichia coli MIO
+/+/V
58
NLF GNRs Info
Found in nature Mostly in Soil and Water GNR or GNCB Do NOT ferment glucose or other carbohydrates KIA = AK/NC Produces weak acids OF Media
59
Pseudomonads
Produces pigments Pyocyanin - blue-green color Pyorubin - red/red-brown Pyoveridin - fluorescent
60
Pseudomonas fluorescens/putida
Oxidase = Positive Fluorescens Gel = Positive Putida Gel = Negative Pyoveriden No Growth at 42 C
61
Stenotrophomonas maltophilia Morphology
Third Most Common Lavender-green Smells like ammonia NLF: ppt at 24 hrs, tan at 48 hrs
62
Stenotrophomonas maltophilia Biochemical Reactions
Oxidase = Negative Catalase = Positive Oxidizes Glucose and Maltose
63
Stenotrophomonas maltophilia LAO
+/-/-
64
Alcaligenes faecalis Morphology
Green Discoloration Green Apple smell
65
Alcaligenes faecalis Biochemical Reactions
Oxidase = Positive Catalase = Positive Motile Reduces Nitrite but not Nitrate
66
Alcaligenes faecalis LAO
-/-/-
67
Burkholderia pseudomallei Morphology
Wrinkled colony Yellow to Brown Beta hemolysis
68
Burkholderia pseudomallei Biochemical Reactions
Oxidase = Positive Nitrate = Positive Motile Oxidizes Glucose, Lactose, Xylose, Mannitol
69
Burkholderia pseudomallei LAO
-/+/-
70
Burkholderia cepacian Morphology
Yellow - sometimes mucoid in CSF Sweet Earthy smell
71
Burkholderia cepacian Biochemical Reactions
Oxidase = Positive (weak) ONPG = Positive Motile Ozidizes Glucose and Lactose
72
Burkholderia cepacian LAO
+/-/-
73
Elizabethkingia meningoseptica Morphology
Yellow colony w/ green-lavender May have fruity smell
74
Elizabethkingia meningoseptica Biochemical Reactions
Oxidase = Positive Catalase = Positive Indole = Positive DNAse= Positive Esculin = Positive H2S = Positive ONPG = Positive
75
Elizabethkingia meningoseptica LAO
-/-/-
76
Chryseomonas or Pseudomonas oryzihabitans Morphology
Yellow Dry and wrinkled No Growth at 42 C
77
Chryseomonas or Pseudomonas oryzihabitans Biochemical Reactions
Nitrite = Negative Oxidizes Glucose, Maltose, Xylose
78
Chryseomonas or Pseudomonas oryzihabitans LAO
-/-/-
79
Moraxella species Info
GNCB MAC: Poor to NG Oxidase = Positive Catalase = Positive Nonmotile Does not Oxidize Glucose
80
Oligella species
Oligella ureolytica Oligella ureothralis
81
Oligella ureolytica
Oxidase = Positive Catalase = Negative Urea = Positive Nitrate = Positive Motile
82
Oligella ureothralis
Oxidase = Positive Catalase = Negative Urea = Negative Nitrate = Negative Nitrite = Positive Nonmotile
83
Oligella species LAO
-/-/-
84
Citrbacter koseri
Citrate = Positive Lyine = Negative MIO +/+/+
85
Enterics
Many are Normal Enteric Flora (NEF) Cause infections in other areas of the body, especially in sterile body sites
86
Enteric Pathogens
Salmonella Shigella Yersinia enterocolitica E.coli O157:H7
87
Enteric Pathogens that are not in Enterobacteriacea
Campylobacter species Aeromonas Virbios Plesiomonas - Now categorized as Enterobacteriacea
88
Antigenic Structure: Salmonella and Shigella serotyping
O = Somatic Antigen H = Flagellar Antigens K = Capsular Antigens Vi = Found in some highly virulent strains of Salmonella
89
Organisms are arranged in tribes
Escherichieae Edwardsielleae Salmonellae Citrobactereae Klebsielleae Proteeae Yersineae
90
Escherichieae Tribe
E.coli O157:H7 (sorbitol negative) Enterotoxigenic E.coli Enteropathogenic E.coli Enteroaggregative E.coli
91
Escherichia coli Diseases
UTIs Bacteremia Pneumonia Wound infections Meningitis (newborns) Diarrhea
92
Shigella species Disease
Bacterial diarrhea Causes very severe GI symptoms
93
Edwardsiella tarda Diseases
** Carried by turtles and reptiles ** Diarrhea Wound infections Bacteremia
94
Salmonella species Disease
Acute gastroenteritis Typhoid Fever
95
Citrobacter species Disease
Similar to Salmonella
96
Klebsiella species Diseases
Pneumonia UTI Septicemia
97
Proteeae Tribe (Proteus, Providencia, Morganella) Diseases
Urine Wounds Blood
98
Yersinia pestis
Very non-reactive Urea = Negative ONPG = Positive MIO = -/-/-
99
Yersinia species Diseases
Yersinia enterocolitica = Causes GI tract infection Yersinia pestis = Plague

Microbiology (9 decks)

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