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Microbiology > Enteric and NLF GNRs > Flashcards

Enteric and NLF GNRs Flashcards

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Biology 101 flashcards
1
Q

Citrobacter freundii

A

Citrate = Positive
Lysine = Negative
ONPG = Positive
KIA = A/AK/A

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2
Q

Citrobacter freundii Lactose Fermentation

A

Mostly NLF
Some subspecies may be LF

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3
Q

Citrobacter freundii MIO

A

+/-/-

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4
Q

Serratia marcescens

A

ONPG = Positive
DNAse = Positive
Gel = Positive
Ara = Negative
KIA = AK/A

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5
Q

Serratia marcescens Morphology

A

BAP colonies = Pink to Red
MAC = Dark Pink to Red
NLF

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6
Q

Serratia marcescens MIO

A

+/-/+

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7
Q

Yersinia pestis

A

NLF
ONPG = Positive
Urea = Negative
Ornithine = Negative
KIA = AK/A

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8
Q

Yersinia pestis MIO

A

-/-/-

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9
Q

Pseudomonas aeruginosa

A

MOST COMMON NLF
Oxidase = Positive
Catalase = Positive
Non-motile
Growth at 42 C
Nitrate = Positive
Gel = Positive
OF/O = Positive
OF/F = Negative

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10
Q

Pseudomonas aeruginosa Morphology

A

Beta Hemolytic
Pyocyanin
Grape or Tortilla Scent
NLF

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11
Q

Pseudomonas aeruginosa LAO

A

-/+/-

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12
Q

Pseudomonas stutzeri Morphology

A

Yellow/Tan
Wrinkled
Leathery
NLF

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13
Q

Pseudomonas stutzeri

A

Oxidase = Positive
Motile
Growth at 42 C
Oxidizes Glucose, Lactose, Maltose, and Fructose
Reduces Nitrate to N2

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14
Q

Pseudomonas stutzeri LAO

A

-/-/-

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15
Q

Acinetobacter species

A

Gram Stain = GNCB
Acinetobacter baumannii
Acinetobacter lwoffi

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16
Q

Acinetobacter baumannii Morphology

A

NLF
Mauve Appearance on MAC

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17
Q

Acinetobacter baumannii

A

Oxidase = Negative
Catalase = Negative
Nonmotile
Oxidizes Glucose and Xylose

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18
Q

Acinetobacter baumannii LAO

A

-/-/-

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19
Q

Acinetobacter lwoffi

A

Oxidase = Negative
Nitrate = Negative
Nonmotile

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20
Q

Acinetobacter lwoffi LAO

A

-/-/-

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21
Q

PDA + Organisms

A

Proteus species
Morganella morganii
Providencia species

Parts Per Million Mnemonic Device for Proteus, Providencia, Morganella

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22
Q

Proteus species

A

Proteus mirabilis
Proteus vulgaris

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23
Q

Proteus mirabilis

A

PDA = Positive
H2S = Positive
KIA = AK/A

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24
Q

Proteus mirabilis MIO

A

+/-/+

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25
Q

Proteus vulgaris

A

PDA = Positive
H2S = Positive
KIA = AK/A

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26
Q

Proteus vulgaris MIO

A

+/+/-

** Vulgaris is Icky**

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27
Q

Morganella morganii

A

PDA = Positive
H2S = Negative
KIA = AK/A

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28
Q

Morganella morganii MIO

A

+/+/+

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29
Q

Providencia species

A

Providencia rettgeri
Providencia stuartii

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30
Q

Providencia rettgeri

A

PDA = Positive
H2S = Negative
Urea = Positive
Mannitol = Positive
KIA = AK/A

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31
Q

Providencia rettgeri MIO

A

+/+/-

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32
Q

Providencia stuartii

A

PDA = Positive
H2S = Negative
Urea = Negative (30% are Positive)
Mannitol = Negative
KIA = AK/A

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33
Q

Providencia stuartii MIO

A

+/+/-

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34
Q

H2S Positive Organisms

A

Edwardsiella tarda
Citrobacter freundii
Salmonella species

** SEC **

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35
Q

Edwardsiella tarda

A

PDA = Negative
H2S = Positive
Citrate = Negative
ONPG = Negative
KIA = AK/A

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36
Q

Edwardsiella tarda MIO

A

+/+/+

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37
Q

Salmonella species

A

PDA = Negative
H2S = Positive
Citrate = Negative
Lsyine = Positive
KIA = AK/A
Serotype

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38
Q

Salmonella species MIO

A

+/-/+

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39
Q

VP (Voges-Proskauer) Positive Organisms

A

Klebsiella species
Enterobacter species
Serratia species

** KES = Kryptonite Enrages Superman **

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40
Q

Klebsiella species

A

Klebsiella pneumoniae
Klebsiella oxytoca
Klebsiella (Enterobacter) aerogenes

41
Q

Klebsiella pneumoniae

A

PDA = Negative
H2S = Negative
VP = Positive
KIA = A/A

42
Q

Klebsiella pneumoniae MIO

A

-/-/-

43
Q

Klebsiella oxytoca

A

PDA = Negative
H2S = Negative
VP = Positive
KIA = A/A

44
Q

Klebsiella oxytoca MIO

A

-/+/-

** Y is Ideal for Indole **

45
Q

Enterobacter species

A

Enterobacter (Klebsiella aerogenes)
Enterobacter cloacae

46
Q

Enterobacter (Klebsiella) aerogenes

A

PDA = Negative
H2S = Negative
VP = Positive
Lysine = Positive
Arginine = Negative
KIA = A/A

** Better if A had been positive to correspond with aerogenes **

47
Q

Enterobacter (Klebsiella) aerogenes MIO

A

+/-/+

48
Q

Enterobacter cloacae

A

PDA = Negative
H2S = Negative
VP = Positive
Lysine = Negative
Arginine = Positive

49
Q

Enterobacter cloacae MIO

A

+/-/+

50
Q

Non-Motile Enteric Organisms

A

Shigella species
Klebsiella species
Yersinia species

** SKY **

51
Q

Shigella species Serotyping

A

A, B, C, D

Dirty = Shigella dysenteriae (A)
Fingers = Shigella flexneri (B)
Brings = Shigella boydii (C)
Shigella = Shigella sonnei (D)

52
Q

Shigella species A, B, C

A

very nonreactive
ONPG = Negative
Ornithine = Negative
KIA = AK/A

53
Q

Shigella species D

A

ONPG = Positive
Ornithine = Positive
KIA = AK/A

54
Q

Yersinia enterocolitica

A

NLF
Very nonreactive
ONPG = Positive
Motile at 25 C
Ornithine = Positive
Urea = Positive
KIA = AK/A

55
Q

Yersinia enterocolitica MIO

A

-/-/-

56
Q

Escherichia coli

A

Mostly LF (Some are NLF)
VP = Negative
MR = Positive
Lysine = Positive
Arginine = Variable
KIA = A/A
H2S = Negative

57
Q

Escherichia coli MIO

A

+/+/V

58
Q

NLF GNRs Info

A

Found in nature
Mostly in Soil and Water
GNR or GNCB
Do NOT ferment glucose or other carbohydrates
KIA = AK/NC
Produces weak acids
OF Media

59
Q

Pseudomonads

A

Produces pigments
Pyocyanin - blue-green color
Pyorubin - red/red-brown
Pyoveridin - fluorescent

60
Q

Pseudomonas fluorescens/putida

A

Oxidase = Positive
Fluorescens Gel = Positive
Putida Gel = Negative
Pyoveriden
No Growth at 42 C

61
Q

Stenotrophomonas maltophilia Morphology

A

Third Most Common
Lavender-green
Smells like ammonia
NLF: ppt at 24 hrs, tan at 48 hrs

62
Q

Stenotrophomonas maltophilia Biochemical Reactions

A

Oxidase = Negative
Catalase = Positive
Oxidizes Glucose and Maltose

63
Q

Stenotrophomonas maltophilia LAO

A

+/-/-

64
Q

Alcaligenes faecalis Morphology

A

Green Discoloration
Green Apple smell

65
Q

Alcaligenes faecalis Biochemical Reactions

A

Oxidase = Positive
Catalase = Positive
Motile
Reduces Nitrite but not Nitrate

66
Q

Alcaligenes faecalis LAO

A

-/-/-

67
Q

Burkholderia pseudomallei Morphology

A

Wrinkled colony
Yellow to Brown
Beta hemolysis

68
Q

Burkholderia pseudomallei Biochemical Reactions

A

Oxidase = Positive
Nitrate = Positive
Motile
Oxidizes Glucose, Lactose, Xylose, Mannitol

69
Q

Burkholderia pseudomallei LAO

A

-/+/-

70
Q

Burkholderia cepacian Morphology

A

Yellow - sometimes mucoid in CSF
Sweet Earthy smell

71
Q

Burkholderia cepacian Biochemical Reactions

A

Oxidase = Positive (weak)
ONPG = Positive
Motile
Ozidizes Glucose and Lactose

72
Q

Burkholderia cepacian LAO

A

+/-/-

73
Q

Elizabethkingia meningoseptica Morphology

A

Yellow colony w/ green-lavender
May have fruity smell

74
Q

Elizabethkingia meningoseptica Biochemical Reactions

A

Oxidase = Positive
Catalase = Positive
Indole = Positive
DNAse= Positive
Esculin = Positive
H2S = Positive
ONPG = Positive

75
Q

Elizabethkingia meningoseptica LAO

A

-/-/-

76
Q

Chryseomonas or Pseudomonas oryzihabitans Morphology

A

Yellow
Dry and wrinkled
No Growth at 42 C

77
Q

Chryseomonas or Pseudomonas oryzihabitans Biochemical Reactions

A

Nitrite = Negative
Oxidizes Glucose, Maltose, Xylose

78
Q

Chryseomonas or Pseudomonas oryzihabitans LAO

A

-/-/-

79
Q

Moraxella species Info

A

GNCB
MAC: Poor to NG
Oxidase = Positive
Catalase = Positive
Nonmotile
Does not Oxidize Glucose

80
Q

Oligella species

A

Oligella ureolytica
Oligella ureothralis

81
Q

Oligella ureolytica

A

Oxidase = Positive
Catalase = Negative
Urea = Positive
Nitrate = Positive
Motile

82
Q

Oligella ureothralis

A

Oxidase = Positive
Catalase = Negative
Urea = Negative
Nitrate = Negative
Nitrite = Positive
Nonmotile

83
Q

Oligella species LAO

A

-/-/-

84
Q

Citrbacter koseri

A

Citrate = Positive
Lyine = Negative

MIO +/+/+

85
Q

Enterics

A

Many are Normal Enteric Flora (NEF)
Cause infections in other areas of the body, especially in sterile body sites

86
Q

Enteric Pathogens

A

Salmonella
Shigella
Yersinia enterocolitica
E.coli O157:H7

87
Q

Enteric Pathogens that are not in Enterobacteriacea

A

Campylobacter species
Aeromonas
Virbios
Plesiomonas - Now categorized as Enterobacteriacea

88
Q

Antigenic Structure: Salmonella and Shigella serotyping

A

O = Somatic Antigen
H = Flagellar Antigens
K = Capsular Antigens
Vi = Found in some highly virulent strains of Salmonella

89
Q

Organisms are arranged in tribes

A

Escherichieae
Edwardsielleae
Salmonellae
Citrobactereae
Klebsielleae
Proteeae
Yersineae

90
Q

Escherichieae Tribe

A

E.coli O157:H7 (sorbitol negative)
Enterotoxigenic E.coli
Enteropathogenic E.coli
Enteroaggregative E.coli

91
Q

Escherichia coli Diseases

A

UTIs
Bacteremia
Pneumonia
Wound infections
Meningitis (newborns)
Diarrhea

92
Q

Shigella species Disease

A

Bacterial diarrhea
Causes very severe GI symptoms

93
Q

Edwardsiella tarda Diseases

A

** Carried by turtles and reptiles **
Diarrhea
Wound infections
Bacteremia

94
Q

Salmonella species Disease

A

Acute gastroenteritis
Typhoid Fever

95
Q

Citrobacter species Disease

A

Similar to Salmonella

96
Q

Klebsiella species Diseases

A

Pneumonia
UTI
Septicemia

97
Q

Proteeae Tribe (Proteus, Providencia, Morganella) Diseases

A

Urine
Wounds
Blood

98
Q

Yersinia pestis

A

Very non-reactive
Urea = Negative
ONPG = Positive
MIO = -/-/-

99
Q

Yersinia species Diseases

A

Yersinia enterocolitica = Causes GI tract infection
Yersinia pestis = Plague

Microbiology (9 decks)

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