Gram Positive Rods Flashcards
Bacillus cereus Morphology
Large green colonies
Rough or Ground Glass Appearance
Beta hemolysis
GS: GPB with spores
Bacillus cereus Reactions
Catalase = Positive
Oxidase = Positive
Lecithinase = Positive
Penicillin = Resistant
Motile
Bacillus species Reactions
Beta Hemolytic (most)
GS: GPB with spores
Catalase = Negative
Oxidase = Positive
Lecithinase = Negative
Penicillin = Variable
Motile
Listeria moncytogenes Morphology
Narrow zone of Beta Hemolysis
CAMP = Positive (shovel pattern)
GS: Short GPR or GPCB
Listeria monocytogenes Reactions
Catalase = Positive
Bile Esculin = Positive
VP = Positive
Motile only at 25 C
Non-spore forming
Listeria monocytogenes Disease
Fifth most common cause of meningits
Corynebacterium species Info
GS: GPR with pallisades
Skin contaminate
Catalase = Positive
Corynebacterium diphtheriae Morphology
Requires special media
Tinsdale or CTBA = Black colonies/brown with halo
GS: GPR with pallisades
Corynebacterium diphtheriae Reactions
Catalase = Positive
Oxidase = Positive
Nitrate = Positive
Urease = Negative
Elek = Positive
Ferments: Glucose and Maltose
Corynebacterium diphtheriae Disease
Respiratory tract
Skin lesion
Erysipelothrix rhusiopathae Info
Alpha hemolytic
Catalase = Negative
H2S = Positive
Non-motile
** Associated with Pigs **
Lactobacillus species
Alpha hemolytic
Catalase = Negative
H2S = Negative
Arcanobacterium haemolyticum Info
Beta Hemolytic
Catalase = Negative
CAMP = Reverse Positive
GS: Thin GPR
Gardnerella vaginalis Morphology
Beta hemolytic on Human Blood
PPT to tiny clear colonies
V agar: Colonies are yellow - hydrolyzes starch
Gardnerella vaginalis Reactions
GS: Gram Variable Pleomorphic Rods
Causes Clue Cells in Vaginal Wet Preps
40% KOH = Whiff Test (Fishy Odor)
Rhodococcus equi Info
Pink Colonies
GS: GPCB
Partial Acid Fast = Positive
Catalase = Negative
Urease = Positive
Non-motile
Bacillus anthracis Morphology
Non-hemolytic
Medusa head colony
GS: GPB with spores
Bacillus anthracis Reactions
Nitrate = Positive
Lecinthinase = Positive
Non-motile
Bacillus anthracis Disease
1) Cutaneous (MOST COMMON) = Primary lesion, black eschar, secondary spread through lymphatics and septicemia
2) Pulmonary = Higher fatalities, respiratory distress and edema
3) Gastrointestinal (MOST SEVERE) = Rarest form of disease, ingestion of spores from contaminated meat
