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Microbiology > Gram Positive Cocci Identification > Flashcards

Gram Positive Cocci Identification Flashcards

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Biology 101 flashcards
1
Q

Staphylococcus epidermidis Gram Stain

A

GPC in clusters

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2
Q

Staphylococcus epidermidis Biochemical Reactions

A

Catalase = Positive
Coagulase = Negative
LAP = Negative

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3
Q

Staphylococcus epidermidis Sensitivities

A

Novobiocin = Sensitive
Test used for urine samples

Polymyxin B = Reistant

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4
Q

Staphylococcus epidermidis Identification

A

API Staph panel
MALDI
Vitek

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5
Q

Staphylococcus epidermidis Diseases

A

Intravascular Catheter infection
Endocarditis

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6
Q

Staphylococcus saprophyticus Gram Stain

A

GPC in clusters

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7
Q

Staphylococcus epidermidis Biochemical reactions

A

Catalase = Positive
Coagulase = Negative
LAP = Negative

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8
Q

Staphylococcus saprophyticus Novobiocin

A

Resistant
Test used for urine samples

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9
Q

Staphylococcus saprophyticus Identification

A

API Staph panel
MALDI
Vitek

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10
Q

Staphylococcus saprophyticus Diseases

A

UTIs in young adult females that are sexually active

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11
Q

Streptococcus bovis Gram Stain

A

GPC in chains

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12
Q

Streptococcus bovis Biochemical reactions

A

Gamma hemolysis
Catalase = Negative
PYR = Negative
LAP = Positive
Latex = D Group
Bile Esculin = Positive
6.5% NaCl = No Growth

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13
Q

Streptococcus bovis Bacitracin

A

Resistant

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14
Q

Streptococcus bovis Diseases

A

Septecemia
Bacteremia

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15
Q

Streptococcus anginosus Gram Stain

A

Viridans Group
GPC in chains

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16
Q

Streptococcus anginosus Biochemical Reactions

A

Alpha hemolysis (Gamma or Beta sometimes)
Catalase = Negative
PYR = Negative
LAP = Positive
Bile Esculin = Negative
6.5% NaCl = No Growth

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17
Q

Streptococcus anginosus Sensitivities

A

Bacitracin = Resistant
Optochin = Resistant

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18
Q

Streptococcus anginosus Diseases

A

Infection of GI, head, and neck

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19
Q

Streptococcus anginosus Identification

A

Remel Strep Panel
MALDI
VItek

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20
Q

Streptococcus pyogenes Gram Stain

A

GPC in chains

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21
Q

Streptococcus pyogenes Biochemical Reactions

A

Beta hemolysis
Catalase = Negative
PYR = Positive
LAP = Positive
Latex = Group A

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22
Q

Streptococcus pyogenes Bacitracin

A

Zone of 12 mm

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23
Q

Streptococcus pyogenes Disease

A

Strep throat
Skin and Wound Infections
Bacteremia
Pneumonia

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24
Q

Aerococcus viridans Gram Stain

A

GPC in tetrads and pairs

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25
Q

Aerococcus viridans Biochemical Reactions

A

Alpha Hemolysis
Catalase = Negative
PYR = Positive
LAP = Negative
Bile Esculin = Negative
6.5% NaCl = Growth

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26
Q

Aerococcus viridans Sensitivities

A

Bacitracin = Resistant
Optochin = Resistant

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27
Q

Aerococcus viridans Diseases

A

Associated with UTIs

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28
Q

Aerococcus viridans Identification

A

MALDI
Vitek

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29
Q

Enterococcus faecalis Gram Stain

A

GPC in chains (elongated ovals)

30
Q

Enterococcus faecalis Biochemical Reactions

A

Gamma hemolysis (may appear slightly alpha)
Catalase = Negative or weak Positive
PYR = Positive
LAP = Positive
Bile Esculin = Positive
6.5% NaCl = Growth

31
Q

Enterococcus faecalis Diseases

A

Bacteremia
Endocarditis
UTIs
Intra-abdominal infections
Wound and soft tissue infections

32
Q

Enterococcus faecalis Identification

A

Strep Panel
MALDI
Vitek

33
Q

Enterococcus faecium Gram Stain

A

GPC in chains (elongated ovals)

34
Q

Enterococcus faecium Biochemical Reactions

A

Gamma Hemolysis (appears more alpha)
Catalase = Negative
PYR = Positive
LAP = Positive
Bile Esculin = Positive
6.5% NaCl = Growth

35
Q

Enterococcus faecium Diseases

A

Bacteremia
Endocarditis
UTIs
Intra-abdominal infection
Wound and soft tissue infections

36
Q

Enterococcus faecium Identification

A

Strep panel
MALDI
Vitek

37
Q

Enterococcus faecalis/faecium Vancomycin

A

Resistance possible
Must test for VRE

38
Q

Micrococcus luteus Gram Stain

A

GPC in tetrads

39
Q

Micrococcus luteus Biochemical Reactions

A

Yellow
Catalase = Positive
Coagulase = Negative (may give false positive look)

40
Q

Micrococcus luteus Sensitivities

A

Bacitracin = Sensitive
Furazolidone = Resistant
Lysostaphin = Resistant

41
Q

Micrococcus luteus DIseases

A

Associated with immunocompromised patients

42
Q

Micrococcus species Gram Stain

A

GPC in tetrads

43
Q

Micrococcus species Biochemical Reactions

A

Catalase = Positive
Coagulase = Negative
Microdase = Positive

44
Q

Staphylococcus aureus Gram Stain

A

GPC in clusters

45
Q

Staphylococcus aureus Biochemical Reactions

A

Beta Hemolysis
Catalase = Positive
Coagulase = Positive
Mannitol Salt = Ferments

46
Q

Staphylococcus aureus Sensitivities

A

Bacitracin = Resistant
Furazolidone = Sensitive
Lysostaphi = Sensitive

47
Q

Staphylococcus aureus Diseases

A

Abscesses (boils)
Furuncles
Cellulitis
Bacteremia
Pneumonia
Bone and joint infections

48
Q

Staphylococcus aureus Methicillin

A

Resistance is possible
Must always test for MRSA reaction

49
Q

Staphylococcus simulans Gram Stain

A

GPC in clusters

50
Q

Staphylococcus simulans Biochemical Reactions

A

Catalase = Positive
Coagulase = Negative

51
Q

Staphylococcus simulans Sensitivities

A

Novobiocin = Sensitive

52
Q

Staphylococcus simulans Diseases

A

Isolated from septic arthritis

53
Q

Staphylococcus simulans Identification

A

API Staph panel
MALDI
Vitek

54
Q

Streptococcus agalactiae Gram Stain

A

GPC in chains

55
Q

Streptococcus agalactiae Biochemical Reactions

A

Beta hemolysis
Catalase = Negative
PYR = Negative
LAP = Positive
Latex = B Group
CAMP test = Positive

56
Q

Streptococcus agalactiae Diseases

A

Neonatal meningitis
Skin and wound infections
Bacteremia
Pneumoniae
Endocarditis
Osteomyolitis

57
Q

Streptococcus agalactiae Sensitivites

A

Bacitracin = Resistant

58
Q

Streptococcus dysgalactiae Gram Stain

A

GPC in chains

59
Q

Streptococcus dysgalactiae Biochemical Reactions

A

Beta hemolysis
Catalase = Negative
LAP = Positive
Latex = Group C

60
Q

Streptococcus dysgalactiae Diseases

A

Bacteremia
Skin Infections

61
Q

Streptococcus dysgalactiae Sensitivities

A

Bacitracin = Resistant

62
Q

Streptococcus mitis Gram Stain

A

GPC in chains

63
Q

Streptococcus mitis Biochemical Reaction

A

Alpha hemolysis
Catalase = Negative
PYR = Negative
LAP = Positive
Bile Esculin = Negative
6.5% NaCl = No Growth

64
Q

Streptococcus mitis Sensitivities

A

Bacitracin = Resistant
Optochin = Resistant

65
Q

Streptococcus mitis Diseases

A

Endocarditis
Meningitis

66
Q

Streptococcus mitis Identification

A

Remel Strep panel
MALDI
Vitek

67
Q

Streptococcus pneumoniae Gram Stain

A

GPDC - lancet shaped (Gram Positive DiploCocci)

68
Q

Streptococcus pneumoniae Biochemical Reactions

A

Alpha hemolysis
Catalase = Negative
PYR = Negative
LAP = Positive
Deoxycholate = Positive (Lysing of colonies)
Bile Esculin = Positive
6.5% NaCl = No Growth

69
Q

Streptococcus pneumoniae Sensitivities

A

Bacitracin = Resistant
Optochin = Sensitive

70
Q

Streptococcus pneumoniae Diseases

A

Bacteremia
Meningitis
Otitis Media
Sinusitis
Bronchitis
Septic arthritis
Pneumonia

Microbiology (9 decks)

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