Other Techniques Flashcards

1
Q

Electron microscopy provides better resolution or magnification than light microscopy?

A

Resolution

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2
Q

What is the most common fixative- is used in elector microscopy?

A

Glutharaldehyde

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3
Q

What are the 2 types of electron microscopy?

A

Transmission and scanning

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4
Q

What is the main difference between scanning and transmission?

A

Scanning provides a 3d image

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5
Q

What does ihc stand for?

A

Immunohistochemistry

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6
Q

Define immunohistochemistry

A

Affinity of antibodies for specific antigens

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7
Q

What are antigens?

A

Proteins

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8
Q

What type of bonds are formed by ihc?

A

Ab-ag bonds

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9
Q

How does ihc identify?

A

Cell type, tissue/cell component, and pathogens

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10
Q

What 2 ways do we obtain antibodies (AB) from

A

Naturally through immune system, or through immoral cells

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11
Q

What type of antibody does direct ihc tag?

A

Primary

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12
Q

What is an example of direct ihc?

A

Polyclonal

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13
Q

What type of antibody does indirect ihc tag?

A

Secondary

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14
Q

Does IHC with antibodies pick up stain more than IHC with out antibodies?

A

Yes

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15
Q

What is PCR an example of?

A

Nuclei acid detection

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16
Q

What does ish stand for?

A

In situ hybridization

17
Q

What does ish detect?

A

Viral DNA, bacteria, Protozoa, or anytrins with DNA