Other Techniques Flashcards
Electron microscopy provides better resolution or magnification than light microscopy?
Resolution
What is the most common fixative- is used in elector microscopy?
Glutharaldehyde
What are the 2 types of electron microscopy?
Transmission and scanning
What is the main difference between scanning and transmission?
Scanning provides a 3d image
What does ihc stand for?
Immunohistochemistry
Define immunohistochemistry
Affinity of antibodies for specific antigens
What are antigens?
Proteins
What type of bonds are formed by ihc?
Ab-ag bonds
How does ihc identify?
Cell type, tissue/cell component, and pathogens
What 2 ways do we obtain antibodies (AB) from
Naturally through immune system, or through immoral cells
What type of antibody does direct ihc tag?
Primary
What is an example of direct ihc?
Polyclonal
What type of antibody does indirect ihc tag?
Secondary
Does IHC with antibodies pick up stain more than IHC with out antibodies?
Yes
What is PCR an example of?
Nuclei acid detection