Organisation 🫀 Flashcards
What do muscle do and what do they contain that helps them?
Contract and contain protein fibres which can change their lengths
And mitochondria to provide energy for muscle contacting
What is a tissue ?
A group of cells with the same structure and function
What is an organ? Give an example.
A group of tissues that work together for a specific function like the stomach - muscle tissues and glandular tissues that release enzymes
What are organs grouped into and what do they do?
Work together to form organisms
What are the 3 main nutrients In food?
Lipids - fat
Proteins
Carbs
Why do lipids proteins and carbs have to be digested ?
Too large to fit in blood stream
What happens during digestion?
Large molecules are broken down into smaller molecules which can then be absorbed into the blood stream
describe how food is digested.
Food is chews in the mouth 👄 enzymes such as amylase found in saliva defeat the starch and sugars
Food passes down oesophagus onto the stomach and protease produces pepsin which digests proteins
Hydrochloric acid is stored to kill bacteria and give protease the right PH for optimal performance- 2
Churning of muscles of stomach turns food to fluid increased surface area of enzymes to digest
Fluid goes into small intestine and chemicals are realised into it from liver and pancreas
Pancreas produces protease amylase and lipase and releases it to the small intestine
Liver release bile which helps emulsify and break down and speed up digestion of lipids and bile neutralises acid related for the stomach
Goes into small intestines with releases protease lipase and amylase small food molecules are absorbed into the blood stream by diffusion or active transport
Fluid goes into large intestine where water is absorbed into blood stream
Poo leaves the body
What are the products of digestion used for?
To build new carbohydrates lipids and proteins
What is the glucose produced in digestion used for ?
Respiration
What do enzymes do ?
Catalyse ( speed up and does not get used up) chemical reactions
What are enzymes ?
Large protein molecules that has an active site where the substrate attaches to
Explain the lock and key 🔐 model and how Enzymes work
An active site has to be complimentary in order for them to be able to catalyse a reaction
Enzyme breaks down substrate to form new products
What are enzymes ?
Specific
What are proteins broken down by? Where do we find it?
Protease - stomach small intestine and pancreas
What happens when we digest proteins ?
The proteases convert the proteins back to the individual amino acids which are absorbed by the blood stream
Then they join together in a different order to make human proteins
What is starch?
A chain of glucose molecules
What is starch broken down by?
Amylase
What are carbs broken down by? Which enzyme ?
Carbohydrases
What happens when starch is digested?
The chain of glucose is broken down by amylase to produce simple sugars
Where is amylase found ?
Saliva in mouth and pancreatic fluid
What do lipid molecules consist of ?
Molecules of glycerol attached to 3 fatty acids
What are lipids digested by ? Found in ?
Lipase and it separates the glycerol and 3 fatty acids
Pancreas and small intestine
Where is bile made? What does it do to lipids (fats )
In the liver and stored in gall bladder
Speed up digestion but is not an enzyme
Bile emulsifies large lipid droplets into smaller lipid droplets
This increase the SA of lipids and increases the rate of breaking it down
What is Bile’s PH? What does it allow it to do?
Alkaline - neutralises stomach acid creating alkaline conditions in small intestine increasing the rate of digestion of lipids
Why do enzymes activity increase and we increase temp?
Increase KE so there are more collisions of active sites and substrates per second
What is the optimum temperature for enzymes?
The best temp that causes enzymes to work at it’s fastest possible rate max frequency of successful collisions of active sites and substrates (37.4•c)
Why at very high temperatures do enzymes stop 🛑 working?
Because they start to denature deforming the active side and substrates no longer making the complimentary
Enzyme molecules vibrate - changes shape
Enzyme can no longer catalyse reaction
What happens if we make a optimal PH of an enzyme more acidic or alkaline ? Why?
Activity drops to 0 - active site denatures if conditions are too acidic or alkaline
LOOK AT MIND MAP FOR TITRATION CALCULATIONS 🫗
Explain the effect the PH in Amylase practical ?
Place one drop of iodine solution onto the well of the spotting tile
Will turn from orange to blue-black if starch is present
Add starch solutions into test tubes
Amylase and buffer solution - PH 5
2cm^3
Label and place them in a water bath of 30•c and leave them for 10 minutes to reach the correct temp
Combine all the tests tubes and stir with glass rod them immediately but back in water bath and start timing it with stop watch
After 30 seconds add drop of solution using a stirring rod into the drops of iodine in the spotting tiles
The iodine should turn blue-black and that indicates that starch in present
Continue keeping tube in water bath until the iodine stays orange. This indicates that there is no starch present - reaction is complete
Record the time taken for reaction to finish for your results
Replete using different PH buffers like 7 and 8
What are the problems with the PH practical for amylase ?
Taking PH every 30 seconds so we have an approximate time until reaction is compete
We are looking when the iodine stops going orange to blue-black which may be hard to see as it’s gradual
Ask several people and decide if its complete
Describe the food test practice
Grind food using mortar and pestle and make a paste using distilled water
Add food to beakers and add distilled water and still chemicals in food allowing the food to dissolve in the water
Filter solution to remove suspended food particles
How do we test for starch ?
But food solution in test tube - 2cm^3
Add iodine - orange
Iodine will turn orange to blue-black
If there is no starch present iodine will stay orange
How do we test for sugars ?
Add food solution in test tube
Add Benedict solution and place test tube 🧪 in a Becker with hot water and leave it for 5 mins
If sugars are present the Benedict’s solutions should turn blue to green yellow then brick-red 🧱
How can we tell the sugar concentration in a food solution?
Green - little sugar
Yellow - a bit more
Red - a lot
What type of sugars does the Benedict solution only work on?
Reducing sugars like glucose
What will the Benedict’s solution not work on?
Non-reducing sugars - sucrose
How do we test for proteins ?
Add 2cm^3 of food solution then Biuret solution - blue to lilac if protein is present
How do we test for lipids ? Why do we not filter it? What do we do instead?
Make food into paste but don’t filter through as lipid molecules can stick to filter paper
Add ethanol and food solution and gently shake it
Does from with to milky and cloudy
Important that no naked flames are present
How is the small intestine adapted for absorption?
Very long - large SA for absorption of molecules of products of digestion
Interior is covered with millions of villi which increased SA
How is the villi adapted for absorption of molecules in digestion ? (Small intestine )
Covered in microvilli- increases SA allowing more molecules to be absorbed at a time
Good blood supply so the villi rapidly removes products of digestion - increased concentration gradient
Has thin membrane to ensure a short diffusion path
LEADING TO A RAPID RATE OF DIFFUSION
What is produces when amylase breaks down starch ?
Sugar (glucose) and maltose
What does mesophyll do in plants ?
Helps plant photosynthesis
What is epidermal tissue?
Tissues that ones the outer surface of muscles
Give an example of epidermal tissue
Skin and waxy covering of plants
What are the 2 types of tissue ?
Epidermal and muscular
What does the glandular tissue do ?
Produces digestive juices that breaks down stomachs food
What does the muscular tissue do ?
Churns food around
What are the 3 tissues that make the stomach?- organ
Epithermrel glandular and muscular
What does the liver do ?
Produce bile
What type of molecule is an enzyme?
Protein
What are the positives of enzymes
Saves costs as can be used in low temperatures
Doesn’t get used up
What do we use if there are any molecules that cannot be absorbed by the villi by diffusion?
We use active transport
What are the 4 type of blood cells ?
Plasma
Platelets
Red blood cells
White blood cells
Explain the red blood cells
Has a biconcave shape to calmly oxygen - no nucleus - high surface area to carry oxygen
Job to carry oxygen
Has haemoglobin - red pigment- binds with oxygen - oxyhemoglobin
In tissues oxyhemoglobin unbinds with oxygen- releasing oxygen to cells
Explain platelets
Small fragments of cells
Helps clots wounds
Stops bleeding and microorganisms 🦠 entering
Lack of this leads to death
What is plasma ?
Straw coloured liquid that carries everything in the blood
Amino acids
Proteins
Urea
White blood cells
Antitoxins
RBS
Platelets
Antibodies
Co2
What do white blood cells do ?
Defend the body against infection