Organelles and compartmentilization Flashcards

1
Q

What are organelles?

A

Organelles are specialized structures within cells that perform distinct functions necessary for cellular operation and survival.

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2
Q

Which structures are NOT considered organelles?

A

The cell wall, cytoskeleton, and cytoplasm are not considered organelles; they provide support and structure but do not have specific functions like organelles do.

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3
Q

Which structures are considered organelles?

A

Nuclei, vesicles, ribosomes, and the plasma membrane are examples of structures that are classified as organelles.

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4
Q

What is the function of the nucleus in a cell?

A

The nucleus serves as the control center of the cell, housing genetic material (DNA) and coordinating activities such as growth, metabolism, and reproduction.

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5
Q

What is the role of ribosomes in cells?

A

Ribosomes are responsible for protein synthesis, translating messenger RNA (mRNA) into polypeptide chains that fold into functional proteins.

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6
Q

How do vesicles function within a cell?

A

Vesicles transport materials within the cell, helping in processes such as secretion, endocytosis, and intracellular transport of proteins and lipids.

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7
Q

Why is it important to understand the functions of different organelles?

A

Understanding organelle functions helps elucidate how cells operate, communicate, and maintain homeostasis, which is vital for overall organism health.

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8
Q

How did advancements in technology contribute to the study of organelles?

A

The invention of ultracentrifuges enabled scientists to isolate and study individual organelles through cell fractionation, enhancing our understanding of their specific functions.

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9
Q

What is cell fractionation?

A

Cell fractionation is a laboratory technique used to separate cellular components based on size and density, allowing for detailed study of individual organelles.

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10
Q

Why is it significant that organelles are discrete subunits within cells?

A

The compartmentalization provided by organelles allows for specialized environments and conditions necessary for various biochemical processes to occur efficiently and simultaneously within the cell.

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11
Q

What is the advantage of separating the nucleus and cytoplasm in eukaryotic cells?

A

The separation allows for distinct processes of gene transcription in the nucleus and translation in the cytoplasm, enhancing regulation and efficiency.

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12
Q

What occurs during gene transcription?

A

Gene transcription is the process where DNA is transcribed into messenger RNA (mRNA) within the nucleus, preparing it for protein synthesis.

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13
Q

How does post-transcriptional modification of mRNA occur?

A

After transcription, mRNA undergoes modifications such as capping, polyadenylation, and splicing before it exits the nucleus to meet ribosomes in the cytoplasm.

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14
Q

Why is post-transcriptional modification important?

A

These modifications ensure mRNA stability, facilitate its export from the nucleus, and enhance translation efficiency by preparing it for ribosomal recognition.

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15
Q

How does this separation differ in prokaryotic cells?

A

In prokaryotes, transcription and translation occur simultaneously in the cytoplasm since there is no nuclear membrane, allowing mRNA to immediately associate with ribosomes.

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16
Q

What are some consequences of simultaneous transcription and translation in prokaryotes?

A

This allows for rapid protein synthesis but limits the opportunity for mRNA processing and quality control that occurs in eukaryotic cells.

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17
Q

How does compartmentalization benefit eukaryotic cells?

A

Compartmentalization allows for specialized environments conducive to specific biochemical processes, enhancing cellular efficiency and organization.

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18
Q

What role do ribosomes play after mRNA exits the nucleus?

A

Ribosomes translate the processed mRNA into proteins, synthesizing polypeptides based on the genetic code carried by the mRNA.

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19
Q

Why is it significant that eukaryotic cells can modify mRNA before translation?

A

It provides an additional layer of regulation, allowing cells to control gene expression and respond to environmental changes more effectively.

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20
Q

How does understanding compartmentalization contribute to biology?

A

Understanding how compartmentalization functions helps explain cellular organization, regulation of metabolic processes, and evolutionary advantages in complex organisms.

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21
Q

What is compartmentalization in cells?

A

Compartmentalization refers to the division of the cytoplasm into distinct areas or organelles, each specialized for specific biochemical processes.

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22
Q

What is one advantage of compartmentalization regarding metabolite concentration?

A

Compartmentalization allows for the concentration of metabolites and enzymes in specific areas, enhancing the efficiency and speed of biochemical reactions.

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23
Q

How does compartmentalization separate incompatible biochemical processes?

A

By isolating different reactions in distinct compartments, cells can prevent harmful interactions between incompatible processes, ensuring that they can occur simultaneously without interference.

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24
Q

What role do lysosomes play in cellular compartmentalization?

A

Lysosomes contain digestive enzymes that break down waste materials and cellular debris, functioning as specialized compartments for degradation and recycling.

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25
Q

How do phagocytic vacuoles exemplify compartmentalization?

A

Phagocytic vacuoles form when a cell engulfs external particles or pathogens, creating a separate compartment where these materials can be digested without affecting the rest of the cell.

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26
Q

Why is it important for enzymes to be concentrated in specific compartments?

A

Concentrating enzymes in specific compartments increases the likelihood of substrate interaction, facilitating faster reaction rates and more effective metabolic pathways.

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27
Q

How does compartmentalization contribute to cellular organization?

A

Compartmentalization organizes cellular functions into distinct areas, allowing for better regulation and coordination of metabolic activities within the cell.

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28
Q

What happens if biochemical processes are not compartmentalized?

A

Without compartmentalization, conflicting reactions could occur simultaneously, leading to inefficiencies and potential damage to cellular structures due to unwanted interactions.

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29
Q

How does understanding compartmentalization enhance our knowledge of cell biology?

A

Understanding compartmentalization helps explain how cells maintain homeostasis, respond to environmental changes, and carry out complex biochemical processes effectively.

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30
Q

Why is compartmentalization considered an evolutionary advantage?

A

Compartmentalization allows for greater complexity in cellular functions, enabling organisms to adapt to diverse environments and perform specialized tasks that support survival and growth.

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31
Q

What is the primary function of mitochondria?

A

Mitochondria are known as the “powerhouses of the cell,” responsible for producing adenosine triphosphate (ATP) through aerobic cellular respiration.

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32
Q

How does the double membrane structure of mitochondria benefit ATP production?

A

The double membrane creates distinct compartments, with the inner membrane housing the electron transport chain and ATP synthase, optimizing conditions for ATP production.

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33
Q

What is the significance of a small volume of intermembrane space in mitochondria?

A

A small intermembrane space allows for rapid accumulation of protons (H⁺ ions) during electron transport, creating a strong electrochemical gradient essential for ATP synthesis.

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34
Q

How do the cristae enhance mitochondrial function?

A

The large surface area of the cristae (folds of the inner membrane) increases the number of embedded proteins and enzymes involved in the electron transport chain and ATP synthesis.

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35
Q

What role does compartmentalization play in mitochondrial function?

A

Compartmentalization allows for the separation of different metabolic processes, such as the Krebs cycle occurring in the matrix and oxidative phosphorylation occurring across the inner membrane.

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36
Q

What occurs in the mitochondrial matrix?

A

The matrix contains enzymes necessary for the Krebs cycle (citric acid cycle), where acetyl-CoA is oxidized to produce NADH and FADH₂, which are crucial for ATP production.

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37
Q

How does the structure of mitochondria relate to their efficiency in energy production?

A

The combination of a double membrane, extensive cristae, and compartmentalization maximizes surface area and creates optimal conditions for efficient ATP generation.

38
Q

Why is aerobic respiration more efficient than anaerobic processes?

A

Aerobic respiration produces significantly more ATP per glucose molecule than anaerobic processes, due to complete oxidation of glucose in mitochondria.

39
Q

What adaptations do mitochondria have for high-energy demanding cells?

A

Cells with high energy demands, such as muscle cells, have more mitochondria and larger cristae to support increased ATP production required for their functions.

40
Q

How does understanding mitochondrial adaptations contribute to biology?

A

Understanding these adaptations helps explain how cells meet their energy needs, adapt to different metabolic demands, and how dysfunctions in mitochondria can lead to various diseases.

41
Q

What is the primary function of chloroplasts?

A

Chloroplasts are organelles responsible for photosynthesis, converting light energy into chemical energy stored in glucose

42
Q

How does the large surface area of thylakoid membranes benefit photosynthesis?

A

The extensive surface area of thylakoid membranes allows for a greater number of photosystems and electron transport proteins, enhancing the efficiency of light absorption and energy conversion.

43
Q

What are thylakoids?

A

Thylakoids are membrane-bound structures within chloroplasts that contain chlorophyll and other pigments, where the light-dependent reactions of photosynthesis occur.

44
Q

Why is a small volume of fluid inside thylakoids advantageous?

A

A small volume allows for rapid accumulation of protons (H⁺ ions) during the light-dependent reactions, creating a strong proton gradient essential for ATP synthesis through chemiosmosis.

45
Q

What is the role of the Calvin cycle in photosynthesis?

A

The Calvin cycle, occurring in the stroma of chloroplasts, uses ATP and NADPH generated from light-dependent reactions to convert carbon dioxide into glucose.

46
Q

How does compartmentalization benefit chloroplast function?

A

Compartmentalization allows distinct environments for light-dependent reactions in thylakoids and the Calvin cycle in the stroma, optimizing conditions for each process.

47
Q

What are photosystems?

A

Photosystems are complexes of proteins and pigments embedded in thylakoid membranes that capture light energy and initiate the process of electron transport during photosynthesis.

48
Q

How do adaptations in chloroplasts enhance overall photosynthetic efficiency?

A

The combination of large surface area, small thylakoid volume, and compartmentalization allows chloroplasts to maximize light capture and optimize biochemical reactions necessary for glucose production.

49
Q

Why is understanding chloroplast adaptations important in biology?

A

Understanding these adaptations helps explain how plants efficiently convert solar energy into chemical energy, which is fundamental to life on Earth and impacts ecological systems.

50
Q

How do chloroplasts illustrate the concept of compartmentalization in cells?

A

Chloroplasts demonstrate compartmentalization by having distinct structures (thylakoids and stroma) that segregate different stages of photosynthesis, allowing for specialized functions within a single organelle.

51
Q

What is the primary structure of the nuclear envelope?

A

The nuclear envelope consists of a double membrane that surrounds the nucleus, separating it from the cytoplasm.

52
Q

What is the significance of having a double membrane in the nucleus?

A

The double membrane provides a barrier that protects genetic material and regulates the exchange of substances between the nucleus and cytoplasm.

53
Q

Why are nuclear pores essential in the nuclear membrane?

A

Nuclear pores allow for selective transport of molecules such as RNA and proteins between the nucleus and cytoplasm, facilitating communication and regulation.

54
Q

How do nuclear pores function?

A

Nuclear pores are large protein complexes that form channels through which molecules can pass, allowing small molecules to diffuse freely while regulating larger macromolecules.

55
Q

What is one advantage of compartmentalizing transcription and translation?

A

Compartmentalization allows for post-transcriptional modifications of mRNA in the nucleus before it is exported to the cytoplasm for translation, enhancing gene expression regulation.

56
Q

How does the nuclear envelope contribute to cell division?

A

During mitosis and meiosis, the nuclear envelope breaks down into vesicles, allowing chromosomes to segregate properly and ensuring accurate distribution to daughter cells.

57
Q

What happens to the nuclear membrane during cell division?

A

The nuclear membrane disassembles into vesicles, which helps facilitate the separation of duplicated chromosomes during cell division processes.

58
Q

Why is it important for mRNA to be modified before reaching ribosomes?

A

Modifying mRNA (e.g., capping, polyadenylation, splicing) ensures stability and proper recognition by ribosomes, optimizing translation efficiency.

59
Q

How does the double membrane structure enhance nuclear function?

A

The double membrane allows for distinct environments within the nucleus and cytoplasm, enabling specialized processes like transcription in the nucleus and translation in the cytoplasm.

60
Q

Why is understanding the structure and function of the nuclear envelope important in biology?

A

Understanding the nuclear envelope’s role in protecting genetic material and regulating gene expression provides insights into cellular function, development, and disease mechanisms.

61
Q

What are free ribosomes?

A

Free ribosomes are ribosomes that float freely in the cytoplasm and synthesize proteins that are typically retained within the cell.

62
Q

What types of proteins do free ribosomes produce?

A

Free ribosomes primarily produce proteins for use within the cell, such as enzymes and structural proteins.

63
Q

What is the rough endoplasmic reticulum (RER)?

A

The rough endoplasmic reticulum is a membrane-bound organelle studded with ribosomes on its cytoplasmic surface, involved in the synthesis of proteins destined for secretion or for use in membranes.

64
Q

How do membrane-bound ribosomes on the RER differ from free ribosomes?

A

Membrane-bound ribosomes synthesize proteins that are either secreted from the cell or incorporated into cellular membranes, while free ribosomes synthesize proteins that remain in the cytoplasm.

65
Q

What is the significance of the rough appearance of the RER?

A

The rough appearance is due to the presence of ribosomes on its surface, which are actively engaged in protein synthesis.

66
Q

How do proteins synthesized on free ribosomes enter cellular functions?

A

Proteins synthesized on free ribosomes can function directly in metabolic processes, serve as enzymes, or contribute to cellular structure without leaving the cytoplasm.

67
Q

What happens to proteins synthesized by membrane-bound ribosomes?

A

Proteins synthesized by membrane-bound ribosomes enter the lumen of the RER, where they undergo folding and post-translational modifications before being transported to their final destinations.

68
Q

Why is compartmentalization important in protein synthesis?

A

Compartmentalization allows for specialized environments for protein synthesis, folding, and modification, enhancing efficiency and accuracy in producing functional proteins.

69
Q

What role does the RER play in protein quality control?

A

The RER assists in ensuring that only properly folded and modified proteins proceed to their next destinations, preventing misfolded proteins from being secreted or incorporated into membranes.

70
Q

How does understanding the functions of free ribosomes and the rough endoplasmic reticulum contribute to cell biology?

A

Understanding these structures helps elucidate how cells produce and manage proteins, which is essential for maintaining cellular function and responding to environmental changes.

71
Q

What is the primary function of the Golgi apparatus?

A

The Golgi apparatus is responsible for processing, modifying, and packaging proteins and lipids for secretion or delivery to other organelles.

72
Q

How does the Golgi apparatus receive proteins?

A

The Golgi apparatus receives proteins from the rough endoplasmic reticulum (RER) via transport vesicles that bud off from the RER.

73
Q

What modifications occur to proteins in the Golgi apparatus?

A

Proteins undergo various modifications, including glycosylation (addition of carbohydrate groups), phosphorylation, and cleavage, which are essential for their final functional form.

74
Q

How does the structure of the Golgi apparatus facilitate its function?

A

The Golgi apparatus consists of stacked membrane-bound sacs (cisternae) that provide a large surface area for enzymatic reactions and modifications of proteins.

75
Q

What is the significance of compartmentalization within the Golgi apparatus?

A

Compartmentalization allows different enzymes to act on proteins in specific regions, ensuring efficient processing and sorting of proteins based on their final destinations.

76
Q

What happens to proteins after they are processed in the Golgi apparatus?

A

After processing, proteins are packaged into vesicles that transport them to their designated locations, such as secretion outside the cell or delivery to lysosomes.

77
Q

How does the Golgi apparatus play a role in secretion?

A

The Golgi apparatus packages proteins into secretory vesicles that fuse with the plasma membrane, releasing their contents outside the cell through exocytosis.

78
Q

Why is proper functioning of the Golgi apparatus crucial for cells?

A

Proper functioning ensures that proteins are correctly modified and sorted, which is vital for maintaining cellular functions and responding to environmental signals.

79
Q

What can happen if there are defects in the Golgi apparatus?

A

Defects in the Golgi apparatus can lead to improper protein processing, resulting in diseases such as congenital disorders, metabolic syndromes, and certain neurodegenerative diseases.

80
Q

How does understanding the structure and function of the Golgi apparatus contribute to cell biology?

A

Understanding its role in protein processing and secretion helps clarify how cells communicate, maintain homeostasis, and respond to changes in their environment.

81
Q

What are vesicles?

A

Vesicles are small, membrane-bound sacs within cells that transport and store substances, facilitating communication and transport between different cellular compartments.

82
Q

What is the primary function of vesicles in cells?

A

Vesicles play a crucial role in transporting proteins, lipids, and other molecules to their destinations, including secretion outside the cell or delivery to organelles.

83
Q

How are vesicles formed?

A

Vesicles can form by budding off from membranes of organelles such as the endoplasmic reticulum or Golgi apparatus, or during processes like endocytosis.

84
Q

What role does clathrin play in vesicle formation?

A

Clathrin is a protein that coats the cytoplasmic side of the membrane during vesicle formation, helping to shape the membrane into a bud and facilitating the budding process.

85
Q

How does clathrin contribute to the specificity of vesicle transport?

A

Clathrin-coated vesicles are involved in specific transport pathways, ensuring that the correct cargo is selected and packaged for transport to designated locations within or outside the cell.

86
Q

What happens to clathrin after vesicle formation?

A

After vesicle budding, clathrin is often removed once the vesicle is formed, allowing it to be recycled for future vesicle formation.

87
Q

What types of substances can be transported by vesicles?

A

Vesicles can transport a variety of substances, including proteins, lipids, neurotransmitters, hormones, and waste products.

87
Q

Why is compartmentalization important for vesicle function?

A

Compartmentalization allows for the separation of different biochemical processes and prevents unwanted interactions between incompatible substances during transport.

88
Q

How do vesicles facilitate communication between organelles?

A

Vesicles enable the transfer of proteins and lipids between organelles, allowing them to communicate and coordinate their functions effectively.

89
Q

Why is understanding vesicle structure and function important in biology?

A

Understanding vesicles is crucial for comprehending cellular processes such as secretion, endocytosis, and intracellular transport, which are vital for maintaining cellular homeostasis and function.