Gas exchange Flashcards

1
Q

What is gas exchange?

A

Gas exchange is the biological process by which organisms take in oxygen and expel carbon dioxide. This vital function is essential for cellular respiration, allowing cells to produce energy and maintain metabolic processes.

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2
Q

Why is gas exchange considered a vital function in all organisms?

A

Gas exchange is considered a vital function in all organisms because it provides the necessary oxygen for cellular respiration and removes carbon dioxide, a waste product. Without efficient gas exchange, cells cannot generate energy, leading to impaired function and survival.

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3
Q

How does the surface area-to-volume ratio change with increasing organism size?

A

As organisms increase in size, their surface area-to-volume ratio decreases. This means that larger organisms have relatively less surface area available for gas exchange compared to their volume, making it more challenging to meet their respiratory needs.

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4
Q

What challenges do larger organisms face regarding gas exchange?

A

Larger organisms face several challenges regarding gas exchange, including:
- Reduced efficiency in oxygen uptake due to lower surface area relative to volume.
- Increased distance from the center of the organism to the exterior, making diffusion less effective.
- Greater metabolic demands that require more oxygen and efficient removal of carbon dioxide.

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5
Q

How do smaller organisms typically manage gas exchange?

A

Smaller organisms typically manage gas exchange efficiently through direct diffusion across their cell membranes. Their high surface area-to-volume ratio allows for sufficient oxygen uptake and carbon dioxide removal without specialized respiratory structures.

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6
Q

What adaptations do larger organisms develop for effective gas exchange?

A

Larger organisms develop various adaptations for effective gas exchange, including:
- Specialized respiratory structures (e.g., lungs in mammals, gills in fish) that increase surface area.
- Circulatory systems to transport gases quickly throughout the body.
- Mechanisms such as ventilation or pumping to enhance airflow or water flow over respiratory surfaces.

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7
Q

Why is diffusion alone insufficient for gas exchange in larger organisms?

A

Diffusion alone is insufficient for gas exchange in larger organisms because it relies on concentration gradients and can be too slow over greater distances. As size increases, the time required for gases to diffuse across tissues becomes impractical for meeting metabolic demands.

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8
Q

How does the distance from the center of an organism to its exterior affect gas exchange?

A

The distance from the center of an organism to its exterior affects gas exchange by increasing the time it takes for gases to diffuse. In larger organisms, this longer distance can hinder efficient oxygen delivery and carbon dioxide removal, necessitating specialized structures or systems.

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9
Q

What role does the circulatory system play in facilitating gas exchange?

A

The circulatory system plays a crucial role in facilitating gas exchange by transporting oxygen from respiratory surfaces (like lungs or gills) to tissues throughout the body and carrying carbon dioxide from tissues back to these surfaces for exhalation or excretion.

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10
Q

How do environmental factors influence gas exchange in organisms?

A

Environmental factors such as temperature, humidity, and oxygen availability influence gas exchange in organisms. For example, higher temperatures may increase metabolic rates and oxygen demand, while low humidity can affect moisture levels necessary for efficient diffusion across respiratory surfaces.

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11
Q

What are the key properties of effective gas-exchange surfaces?

A

The key properties of effective gas-exchange surfaces include:
- High permeability to gases.
- A thin tissue layer to facilitate diffusion.
- Moisture to maintain gas solubility.
- A large surface area to maximize gas exchange efficiency.

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12
Q

Why is permeability important for gas-exchange surfaces?

A

Permeability is important for gas-exchange surfaces because it allows gases (such as oxygen and carbon dioxide) to diffuse easily across the membrane. High permeability ensures that gases can move rapidly in and out, facilitating efficient respiration.

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13
Q

How does a thin tissue layer enhance gas exchange?

A

A thin tissue layer enhances gas exchange by minimizing the distance that gases must diffuse. The shorter the distance, the faster the rate of diffusion, allowing for more efficient oxygen uptake and carbon dioxide removal.

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14
Q

What role does moisture play in gas exchange?

A

Moisture plays a critical role in gas exchange by dissolving gases, which allows them to diffuse more readily across membranes. In many organisms, respiratory surfaces need to be moist to facilitate the movement of oxygen into cells and carbon dioxide out of cells.

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15
Q

Why is a large surface area beneficial for gas exchange?

A

A large surface area is beneficial for gas exchange because it provides more space for gas molecules to diffuse. This increases the overall rate of gas exchange, allowing organisms to meet their metabolic demands more effectively.

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16
Q

How do alveoli in mammalian lungs exemplify these properties?

A

Alveoli in mammalian lungs exemplify these properties by having thin walls (one cell thick), being moist with a surfactant layer, having a high permeability to gases, and providing a vast surface area due to their numerous and highly branched structure, facilitating efficient gas exchange.

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17
Q

How do gills in fish demonstrate the importance of large surface area?

A

Gills in fish demonstrate the importance of large surface area by having numerous filaments and lamellae that create extensive surface area for oxygen absorption from water. This adaptation allows fish to extract sufficient oxygen even in environments where oxygen levels may be low.

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18
Q

What adaptations do amphibians have for gas exchange that highlight moisture’s role?

A

Amphibians have adaptations such as moist skin that allows for cutaneous respiration (gas exchange through the skin). This reliance on moisture is crucial since their skin must remain wet to facilitate the diffusion of gases directly into the bloodstream.

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19
Q

How does the structure of plant leaves support effective gas exchange?

A

The structure of plant leaves supports effective gas exchange through features such as a large surface area provided by broad leaves, stomata that allow for moisture retention and gas diffusion, and a thin epidermal layer that minimizes diffusion distance.

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20
Q

What challenges might arise if any of these properties are compromised?

A

If any of these properties are compromised (e.g., if the gas-exchange surface becomes thickened or dry), it can lead to reduced efficiency in gas exchange. This may result in insufficient oxygen uptake or inadequate removal of carbon dioxide, ultimately affecting cellular respiration and overall organism health.

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21
Q

What is the significance of maintaining concentration gradients at gas-exchange surfaces?

A

Maintaining concentration gradients at gas-exchange surfaces is crucial for efficient gas exchange, as it allows for the continuous movement of oxygen into the bloodstream and carbon dioxide out of the body. A steep concentration gradient enhances diffusion rates.

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22
Q

How do dense networks of blood vessels contribute to gas exchange?

A

Dense networks of blood vessels contribute to gas exchange by ensuring that a large volume of blood is in close proximity to the exchange surface. This allows for rapid absorption of oxygen and removal of carbon dioxide, helping to maintain concentration gradients.

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23
Q

What role does continuous blood flow play in gas exchange?

A

Continuous blood flow plays a vital role in gas exchange by constantly bringing deoxygenated blood to the exchange surfaces (like lungs or gills) and removing oxygenated blood quickly. This renewal of blood helps maintain high concentration gradients for efficient diffusion.

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24
Q

How do lungs facilitate gas exchange through ventilation?

A

Lungs facilitate gas exchange through ventilation, which involves inhaling fresh air rich in oxygen and exhaling air containing carbon dioxide. This process helps maintain a steep concentration gradient between the air in the alveoli and the blood in the surrounding capillaries.

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25
Q

What is the mechanism of ventilation in fish gills?

A

Fish ventilate their gills by using two skeletal muscle pumps that draw water into the mouth and push it over the gill filaments. This continuous flow of water helps maintain a concentration gradient, allowing for efficient oxygen uptake and carbon dioxide removal.

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26
Q

How does counter-current flow enhance gas exchange efficiency in fish gills?

A

Counter-current flow enhances gas exchange efficiency in fish gills by ensuring that water flows over the gills in one direction while blood flows through the capillaries in the opposite direction. This arrangement maximizes the concentration gradient, allowing for more effective oxygen absorption.

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27
Q

Why is a large surface area important for gas exchange surfaces?

A

A large surface area is important for gas exchange surfaces because it increases the amount of space available for gases to diffuse across. This maximizes the rate of gas exchange, ensuring that sufficient oxygen enters and carbon dioxide exits efficiently.

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28
Q

How does moisture affect gas exchange at respiratory surfaces?

A

Moisture affects gas exchange at respiratory surfaces by dissolving gases, which facilitates their diffusion across membranes. Moist environments help maintain the solubility of gases, enhancing their movement into and out of cells during respiration.

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29
Q

What adaptations do mammalian lungs have to support effective gas exchange?

A

Mammalian lungs have several adaptations to support effective gas exchange, including:
- Alveoli with thin walls to reduce diffusion distance.
- A large surface area due to numerous alveoli.
- A rich supply of capillaries to facilitate rapid gas transfer.

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30
Q

What challenges do aquatic organisms face regarding gas exchange compared to terrestrial organisms?

A

Aquatic organisms face challenges regarding gas exchange due to lower concentrations of dissolved oxygen in water compared to air. They must have specialized structures (like gills) with large surface areas and efficient ventilation mechanisms to extract sufficient oxygen from their environment.

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31
Q

What are the key adaptations of mammalian lungs for gas exchange?

A

Key adaptations of mammalian lungs for gas exchange include:
- The presence of surfactant.
- A branched network of bronchioles.
- Extensive capillary beds surrounding alveoli.
- A high surface area provided by numerous alveoli.

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32
Q

What is the role of surfactant in the alveoli?

A

Surfactant plays a crucial role in the alveoli by reducing surface tension at the air-liquid interface. This prevents alveolar collapse during exhalation, ensuring that the alveoli remain open and available for gas exchange.

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33
Q

How is surfactant produced in the lungs?

A

Surfactant is produced by type II alveolar cells as a mixture of phospholipids and proteins. It is secreted into the alveoli, where it forms a thin film that lowers surface tension, facilitating efficient gas exchange.

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34
Q

Why is a branched network of bronchioles important for gas exchange?

A

A branched network of bronchioles is important for gas exchange because it increases airflow throughout the lungs, ensuring that air reaches all alveoli. This extensive branching maximizes the surface area available for gas exchange.

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35
Q

How do extensive capillary beds enhance gas exchange in the lungs?

A

Extensive capillary beds enhance gas exchange by providing a large surface area for blood to come into close contact with alveolar air. This proximity allows for efficient diffusion of oxygen into the blood and carbon dioxide out of the blood.

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36
Q

What is the significance of having a high surface area in mammalian lungs?

A

The high surface area in mammalian lungs, created by approximately 300 million alveoli, allows for a greater volume of gases to be exchanged simultaneously. This adaptation ensures that sufficient oxygen can be absorbed and carbon dioxide can be expelled efficiently.

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37
Q

How does moisture in the alveoli facilitate gas exchange?

A

Moisture in the alveoli facilitates gas exchange by dissolving gases, allowing them to diffuse more easily across the thin alveolar membrane. This moist environment is essential for effective oxygen uptake and carbon dioxide removal.

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38
Q

What happens if there is a deficiency of surfactant in the lungs?

A

A deficiency of surfactant in the lungs can lead to conditions such as neonatal respiratory distress syndrome, where alveoli collapse (atelectasis) due to high surface tension. This results in impaired gas exchange and can be life-threatening, particularly in premature infants.

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39
Q

How does the structure of alveoli contribute to their function in gas exchange?

A

The structure of alveoli contributes to their function in gas exchange by being extremely thin-walled (one cell thick), which minimizes diffusion distance for gases. Their spherical shape also maximizes surface area while minimizing tissue volume.

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40
Q

Why are adaptations like surfactant and extensive capillary networks critical for mammals?

A

Adaptations like surfactant and extensive capillary networks are critical for mammals because they enable efficient gas exchange necessary to meet high metabolic demands. These adaptations allow mammals to thrive in various environments and support activities requiring rapid oxygen delivery, such as exercise.

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41
Q

What is the primary role of the diaphragm in ventilation?

A

The primary role of the diaphragm in ventilation is to contract and flatten during inhalation, increasing the volume of the thoracic cavity, which lowers pressure in the lungs and allows air to flow in. During exhalation, it relaxes and returns to its dome shape, pushing air out of the lungs.

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42
Q

How do intercostal muscles assist in the process of breathing?

A

Intercostal muscles assist in breathing by contracting to elevate and expand the rib cage during inhalation. This action increases the volume of the thoracic cavity further, enhancing airflow into the lungs. They also help lower the rib cage during exhalation, aiding in pushing air out.

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43
Q

What happens to the thoracic cavity during inspiration?

A

During inspiration, the diaphragm contracts and moves downward, while the external intercostal muscles contract to lift and expand the ribs. This combination increases the volume of the thoracic cavity, resulting in a decrease in intra-alveolar pressure that allows air to flow into the lungs.

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44
Q

Describe the process of expiration.

A

Expiration is primarily a passive process where the diaphragm and intercostal muscles relax after inhalation. The elastic recoil of lung tissue decreases thoracic cavity volume, increasing intrapulmonary pressure above atmospheric pressure, which forces air out of the lungs.

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45
Q

How does pleural fluid contribute to lung ventilation?

A

Pleural fluid creates a cohesive bond between the lungs and thoracic wall, allowing for lung expansion during inhalation. As the thoracic cavity expands due to diaphragm and intercostal muscle activity, this fluid helps ensure that lung movement is synchronized with chest wall movement.

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46
Q

What is the function of abdominal muscles during ventilation?

A

Abdominal muscles assist in ventilation by providing support during forced exhalation. When these muscles contract, they push up against the diaphragm, helping to expel air more forcefully from the lungs during activities like exercise or coughing.

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47
Q

Explain how changes in pressure drive pulmonary ventilation.

A

Changes in pressure drive pulmonary ventilation by creating a pressure gradient. During inhalation, atmospheric pressure is greater than intra-alveolar pressure due to increased thoracic volume. Conversely, during exhalation, intrapulmonary pressure exceeds atmospheric pressure, causing air to flow out.

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48
Q

What role do external intercostal muscles play specifically?

A

External intercostal muscles play a specific role during inhalation by contracting to raise and expand the rib cage. This action increases thoracic volume and decreases intra-alveolar pressure, facilitating air intake into the lungs.

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49
Q

How does aging affect diaphragm function?

A

Aging can affect diaphragm function by reducing its strength and efficiency due to factors such as muscle atrophy or changes in lung elasticity. This can lead to decreased respiratory capacity and difficulties in maintaining effective ventilation.

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50
Q

Why is proper functioning of respiratory muscles essential for effective ventilation?

A

Proper functioning of respiratory muscles is essential for effective ventilation because they work together to regulate airflow into and out of the lungs. Any impairment in these muscles can lead to inadequate gas exchange, reduced oxygen availability, and overall respiratory distress.

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51
Q

What is tidal volume (TV)?

A

Tidal volume (TV) is the amount of air that is inhaled or exhaled during normal, relaxed breathing. The average tidal volume is about 500 mL for both men and women.

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52
Q

How is tidal volume measured?

A

Tidal volume can be measured using a spirometer, which records the volume of air displaced during inhalation and exhalation. Various methods, including dry and wet spirometers, can accurately determine tidal volume.

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53
Q

What is vital capacity (VC)?

A

Vital capacity (VC) is the maximum amount of air that can be exhaled after a maximum inhalation. It represents the total usable volume of the lungs and is typically around 4600 mL in males and 3400 mL in females.

54
Q

How do you calculate vital capacity?

A

Vital capacity can be calculated by summing the inspiratory reserve volume (IRV), tidal volume (TV), and expiratory reserve volume (ERV). The formula is VC = IRV + TV + ERV.

55
Q

What is inspiratory reserve volume (IRV)?

A

Inspiratory reserve volume (IRV) is the additional amount of air that can be inhaled beyond the tidal volume during a forceful breath. The average IRV is approximately 3000 mL in males and 2100 mL in females.

56
Q

What is expiratory reserve volume (ERV)?

A

Expiratory reserve volume (ERV) is the additional amount of air that can be forcibly exhaled after a normal tidal expiration. The average ERV is about 1100 mL in males and 800 mL in females.

57
Q

What equipment is commonly used to measure lung volumes?

A

Common equipment used to measure lung volumes includes spirometers, body plethysmographs, and multi-breath dilution techniques using gases like helium or nitrogen. Each method has its advantages for measuring different lung volumes.

58
Q

Why are lung volume measurements important?

A

Lung volume measurements are important for diagnosing respiratory conditions, assessing lung function, and monitoring treatment progress. They help identify ventilatory defects and provide insights into overall respiratory health.

59
Q

What factors can affect lung volumes?

A

Factors that can affect lung volumes include age, sex, height, physical fitness, body composition, and environmental conditions such as altitude. Taller individuals typically have larger lung volumes compared to shorter individuals.

60
Q

How do residual volume (RV) and functional residual capacity (FRC) relate to lung volumes?

A

Residual volume (RV) is the amount of air remaining in the lungs after a maximal exhalation, typically around 1200 mL in males. Functional residual capacity (FRC) is the total amount of air left in the lungs after a normal expiration, calculated as FRC = ERV + RV.

61
Q

What is the function of the waxy cuticle in leaves?

A

The waxy cuticle is a protective layer on the surface of leaves that reduces water loss through evaporation. It helps maintain moisture within the leaf while allowing light to penetrate for photosynthesis.

62
Q

How does the epidermis contribute to gas exchange in leaves?

A

The epidermis is a single layer of cells that protects the leaf and helps regulate gas exchange. It is transparent, allowing light to pass through, and it contains stomata, which are openings for gas exchange.

63
Q

What role do air spaces play in leaf gas exchange?

A

Air spaces within the leaf’s mesophyll allow for the diffusion of gases (oxygen and carbon dioxide) throughout the leaf. These spaces increase the surface area available for gas exchange and facilitate efficient movement of gases to and from cells.

64
Q

What is the function of spongy mesophyll in leaves?

A

The spongy mesophyll consists of loosely packed cells with large air spaces, providing a site for gas exchange and facilitating the diffusion of carbon dioxide to photosynthesizing cells. Its structure maximizes surface area while minimizing resistance to gas movement.

65
Q

How do stomatal guard cells regulate gas exchange?

A

Stomatal guard cells control the opening and closing of stomata, which are small pores on the leaf surface. By changing shape in response to environmental conditions (e.g., light, humidity), guard cells regulate gas exchange and water loss, optimizing photosynthesis and transpiration.

66
Q

What is the significance of veins in leaf structure?

A

Veins in leaves contain xylem and phloem tissues that transport water, nutrients, and sugars throughout the plant. They provide structural support to the leaf while ensuring that all parts receive essential resources for photosynthesis and growth.

67
Q

How does leaf structure maximize photosynthesis?

A

Leaf structure maximizes photosynthesis by optimizing light capture (through broad, flat surfaces), facilitating gas exchange (via stomata and air spaces), and ensuring efficient transport of water and nutrients (through veins). These adaptations work together to enhance overall photosynthetic efficiency.

68
Q

Why is it important for leaves to have a large surface area?

A

A large surface area is important for leaves because it increases exposure to sunlight, enhancing light absorption for photosynthesis. Additionally, a larger surface area allows for more stomata, facilitating greater gas exchange with the atmosphere.

69
Q

How do environmental factors influence stomatal behavior?

A

Environmental factors such as light intensity, humidity, temperature, and carbon dioxide concentration influence stomatal behavior. For example, stomata tend to open in bright light to allow more carbon dioxide in for photosynthesis while closing during dry conditions to reduce water loss.

70
Q

What adaptations help prevent excessive water loss in leaves while maintaining gas exchange?

A

Adaptations that help prevent excessive water loss while maintaining gas exchange include:
- The waxy cuticle that reduces evaporation.
- Stomatal regulation by guard cells that control opening size.
- The spongy mesophyll structure that allows for efficient gas diffusion without compromising moisture retention.

71
Q

What are the main tissues found in a transverse section of a dicotyledonous leaf?

A

The main tissues found in a transverse section of a dicotyledonous leaf include:
- Epidermis (upper and lower)
- Mesophyll (palisade and spongy)
- Vascular tissue (xylem and phloem)
- Waxy cuticle

72
Q

Where is the upper epidermis located in a dicotyledonous leaf?

A

The upper epidermis is located on the top surface of the leaf. It serves as a protective layer and is usually covered by a waxy cuticle to minimize water loss.

73
Q

What is the role of the palisade mesophyll?

A

The palisade mesophyll is located just beneath the upper epidermis and consists of tightly packed cells rich in chloroplasts. Its primary role is to maximize light absorption for photosynthesis.

74
Q

Describe the structure and function of spongy mesophyll.

A

The spongy mesophyll is located beneath the palisade mesophyll and consists of loosely arranged cells with large air spaces. This structure facilitates gas exchange (carbon dioxide and oxygen) and allows for efficient diffusion within the leaf.

75
Q

What is the function of the lower epidermis?

A

The lower epidermis is located on the underside of the leaf and contains stomata, which regulate gas exchange. It also helps protect the leaf while allowing for transpiration and gas exchange.

76
Q

How are xylem and phloem arranged in a dicotyledonous leaf?

A

In a dicotyledonous leaf, xylem and phloem are arranged together in vascular bundles (veins). Xylem typically appears on the upper side of the bundle, transporting water and minerals from the roots, while phloem is located on the lower side, transporting sugars produced during photosynthesis.

77
Q

What is the significance of vascular bundles in leaves?

A

Vascular bundles are significant in leaves because they provide structural support, transport water, nutrients, and sugars throughout the plant, and facilitate communication between different parts of the plant.

78
Q

How does the distribution of tissues in a leaf contribute to its overall function?

A

The distribution of tissues in a leaf contributes to its overall function by optimizing photosynthesis (palisade mesophyll for light absorption), facilitating gas exchange (spongy mesophyll and stomata), providing structural support (vascular bundles), and minimizing water loss (epidermis and cuticle).

79
Q

What should be included when drawing a plan diagram of a dicotyledonous leaf?

A

When drawing a plan diagram of a dicotyledonous leaf, include labeled sections for:
- Upper epidermis
- Waxy cuticle
- Palisade mesophyll
- Spongy mesophyll
- Lower epidermis
- Stomata
- Vascular bundles (xylem and phloem)

80
Q

Why is it important for students to understand tissue distribution in leaves?

A

Understanding tissue distribution in leaves is important for students because it provides insights into how leaves function in photosynthesis, gas exchange, and nutrient transport. This knowledge is fundamental to understanding plant biology and ecology.

81
Q

What is transpiration in plants?

A

Transpiration is the process of water vapor loss from the stems and leaves of plants, primarily through small openings called stomata. It is a critical consequence of gas exchange, facilitating water movement from roots to leaves.

82
Q

How does gas exchange relate to transpiration?

A

Gas exchange relates to transpiration because the opening of stomata for carbon dioxide uptake during photosynthesis also allows water vapor to escape. Thus, transpiration is an inevitable consequence of the gas exchange process in leaves.

83
Q

What role do stomata play in transpiration?

A

Stomata are pores located on the underside of leaves that regulate gas exchange and transpiration. They open to allow carbon dioxide in for photosynthesis and simultaneously enable the loss of water vapor from the leaf.

84
Q

How does light intensity affect the rate of transpiration?

A

Light intensity affects the rate of transpiration because higher light levels stimulate stomatal opening, increasing gas exchange and water loss. As photosynthesis rates rise in bright light, transpiration rates also tend to increase.

85
Q

What effect does temperature have on transpiration?

A

Temperature affects transpiration by increasing the rate of evaporation and diffusion. Higher temperatures generally lead to increased transpiration rates; however, if temperatures become excessively high, stomata may close to conserve water.

86
Q

How does humidity influence transpiration rates?

A

Humidity influences transpiration rates inversely; as relative humidity increases, the rate of transpiration decreases. High humidity reduces the concentration gradient between the inside of the leaf and the surrounding air, slowing water vapor loss.

87
Q

What impact does wind velocity have on transpiration?

A

Wind velocity impacts transpiration by increasing its rate. Wind removes water vapor from around the leaf surface, enhancing the concentration gradient and promoting more rapid diffusion of water vapor out through stomata.

88
Q

How does water availability affect transpiration?

A

Water availability directly affects transpiration rates; when soil moisture is abundant, plants can maintain high rates of transpiration. Conversely, limited water availability can lead to reduced transpiration as plants close their stomata to conserve moisture.

89
Q

What internal factors can influence transpiration?

A

Internal factors influencing transpiration include leaf area (larger areas allow for more stomata), leaf structure (thicker cuticles reduce water loss), and the number and distribution of stomata on leaf surfaces.

90
Q

Why is understanding transpiration important for plant health?

A

Understanding transpiration is important for plant health because it plays a key role in nutrient transport, temperature regulation, and maintaining turgor pressure. Proper management of transpiration helps ensure optimal growth and resilience against environmental stressors.

91
Q

What is stomatal density?

A

Stomatal density refers to the number of stomata (pores) per unit area on a leaf’s surface. It is an important characteristic that influences a plant’s gas exchange and transpiration rates.

92
Q

How can stomatal density be measured using micrographs?

A

Stomatal density can be measured using micrographs by capturing images of leaf sections at high magnification. The number of stomata visible in a defined area (e.g., mm²) is then counted to calculate the stomatal density.

93
Q

What is the purpose of performing leaf casts in measuring stomatal density?

A

Performing leaf casts involves creating a mold of the leaf surface using materials like silicone or clear nail polish. Once dried, the cast can be examined under a microscope to count the number of stomata, providing a clear and accurate measurement of stomatal density.

94
Q

Why is it important to repeat measurements when determining stomatal density?

A

It is important to repeat measurements when determining stomatal density to increase the reliability and accuracy of the data. Repeated counts help account for variability in biological material and provide a more representative average for stomatal density.

95
Q

How does high power magnification aid in counting stomata?

A

High power magnification aids in counting stomata by providing a clearer and more detailed view of the leaf surface. This allows for more accurate identification and counting of individual stomata, reducing the likelihood of errors.

96
Q

What factors can influence stomatal density in plants?

A

Factors that can influence stomatal density include environmental conditions (such as light, humidity, and CO₂ concentration), plant species, leaf age, and water availability. These factors can lead to adaptations in stomatal density for optimal gas exchange.

97
Q

How can variability in biological material affect stomatal density measurements?

A

Variability in biological material can affect stomatal density measurements due to differences in leaf structure, developmental stage, or environmental exposure among individual plants. This variability necessitates multiple trials to obtain reliable data.

98
Q

What is the significance of understanding stomatal density in plant biology?

A

Understanding stomatal density is significant in plant biology because it provides insights into how plants adapt to their environment, manage water loss, and optimize gas exchange for photosynthesis. It also has implications for studies on climate change and plant responses to environmental stressors.

99
Q

What is a common method for calculating stomatal density from micrographs?

A

A common method for calculating stomatal density from micrographs involves counting the number of stomata within a defined area (e.g., 1 mm²) and then expressing this count as the number of stomata per unit area. This allows for standardized comparisons across samples.

100
Q

How does replicating trials enhance scientific research on stomatal density?

A

Replicating trials enhances scientific research on stomatal density by providing a more robust dataset that accounts for variability and potential outliers. It strengthens conclusions drawn from the data and increases confidence in the results obtained from the study.

101
Q

What is the primary function of haemoglobin in the body?

A

The primary function of haemoglobin is to transport oxygen from the lungs to tissues throughout the body and to carry carbon dioxide from tissues back to the lungs for exhalation.

102
Q

How does foetal haemoglobin (HbF) differ from adult haemoglobin (HbA)?

A

Foetal haemoglobin (HbF) differs from adult haemoglobin (HbA) in its structure; HbF contains two alpha chains and two gamma chains, while HbA has two alpha chains and two beta chains. This structural difference gives HbF a higher affinity for oxygen.

103
Q

Why is a higher affinity for oxygen important for foetal haemoglobin?

A

A higher affinity for oxygen is important for foetal haemoglobin because it allows the fetus to effectively extract oxygen from maternal blood in the placenta, even under low oxygen tension conditions.

104
Q

What is cooperative binding in the context of haemoglobin?

A

Cooperative binding refers to the phenomenon where the binding of one oxygen molecule to a haemoglobin molecule increases the likelihood of additional oxygen molecules binding. This results in a sigmoidal (S-shaped) oxygen dissociation curve, enhancing oxygen uptake and release.

105
Q

How does allosteric binding of carbon dioxide affect haemoglobin’s function?

A

Allosteric binding of carbon dioxide affects haemoglobin’s function by promoting the release of oxygen. When carbon dioxide binds to haemoglobin, it stabilizes the deoxygenated form, facilitating oxygen unloading in tissues where it is most needed.

106
Q

What is the Bohr effect, and how does it relate to gas exchange?

A

The Bohr effect describes how increased levels of carbon dioxide and decreased pH (more acidic conditions) in tissues promote the release of oxygen from haemoglobin. This mechanism enhances oxygen delivery to metabolically active tissues that produce more carbon dioxide.

107
Q

How does foetal haemoglobin’s oxygen dissociation curve compare to that of adult haemoglobin?

A

The oxygen dissociation curve for foetal haemoglobin is shifted to the left compared to that of adult haemoglobin. This means that at any given partial pressure of oxygen, HbF has a higher percentage saturation than HbA, allowing for more efficient oxygen uptake.

108
Q

What happens to foetal haemoglobin after birth?

A

After birth, foetal haemoglobin (HbF) gradually decreases as adult haemoglobin (HbA) production increases. By about six months of age, HbA becomes the predominant form of haemoglobin in the bloodstream.

109
Q

Why is it beneficial for adult haemoglobin to have a lower affinity for oxygen compared to foetal haemoglobin?

A

It is beneficial for adult haemoglobin to have a lower affinity for oxygen because this allows for efficient release of oxygen into tissues that are actively respiring and require more oxygen. It ensures that oxygen can be delivered effectively where it is needed most.

110
Q

What role do structural differences in hemoglobin play in its function?

A

Structural differences in hemoglobin, such as the types of globin chains present, influence its affinity for oxygen and carbon dioxide. These adaptations enable different forms of hemoglobin (like HbF and HbA) to meet the specific respiratory needs of fetuses and adults effectively.

111
Q

What is the Bohr effect?

A

The Bohr effect is a physiological phenomenon where an increase in carbon dioxide concentration leads to a decrease in haemoglobin’s affinity for oxygen, resulting in enhanced oxygen release in respiring tissues.

112
Q

How does carbon dioxide influence the oxygen dissociation curve?

A

Carbon dioxide influences the oxygen dissociation curve by causing it to shift to the right when CO₂ levels increase. This shift indicates that at any given partial pressure of oxygen, haemoglobin is less saturated with oxygen, promoting oxygen unloading.

113
Q

What happens to blood pH when carbon dioxide levels rise?

A

When carbon dioxide levels rise, it combines with water to form carbonic acid, which dissociates into bicarbonate and hydrogen ions. This process lowers the pH of the blood, making it more acidic.

114
Q

Why is the Bohr effect beneficial for actively respiring tissues?

A

The Bohr effect is beneficial for actively respiring tissues because it facilitates greater oxygen release in areas where carbon dioxide production is high, ensuring that tissues receive adequate oxygen to meet metabolic demands.

115
Q

What role do hydrogen ions play in the Bohr effect?

A

Hydrogen ions bind to haemoglobin, stabilizing its deoxygenated form (T-state), which promotes the release of oxygen. The presence of H⁺ ions thus enhances the Bohr effect by further reducing haemoglobin’s affinity for oxygen.

116
Q

How does increased metabolism in tissues affect haemoglobin’s oxygen release?

A

Increased metabolism in tissues raises carbon dioxide levels and decreases pH, which triggers the Bohr effect. This results in haemoglobin releasing more oxygen where it is most needed, such as in active muscles during exercise.

117
Q

What physiological changes occur during strenuous activity related to the Bohr effect?

A

During strenuous activity, muscle cells produce more carbon dioxide and lactic acid, leading to lower pH levels. This stimulates the Bohr effect, enhancing oxygen delivery from haemoglobin to these metabolically active tissues.

118
Q

How does the Bohr effect help maintain homeostasis during exercise?

A

The Bohr effect helps maintain homeostasis during exercise by ensuring that increased oxygen demand in active tissues is met by promoting efficient oxygen unloading from haemoglobin, allowing for sustained energy production through respiration.

119
Q

What is the significance of the rightward shift of the oxygen dissociation curve?

A

The rightward shift of the oxygen dissociation curve signifies that haemoglobin releases more oxygen at higher carbon dioxide concentrations and lower pH levels. This adaptation allows for better oxygen delivery to tissues undergoing high metabolic activity.

120
Q

Who first described the Bohr effect and when?

A

The Bohr effect was first described by Danish physiologist Christian Bohr in 1904. His research highlighted how changes in carbon dioxide concentration and pH affect haemoglobin’s ability to bind and release oxygen.

121
Q

What does the oxygen dissociation curve represent?

A

The oxygen dissociation curve represents the relationship between the partial pressure of oxygen (pO₂) and the saturation of haemoglobin with oxygen. It illustrates how readily haemoglobin binds and releases oxygen at different oxygen concentrations.

122
Q

What is the shape of the oxygen dissociation curve, and why is it significant?

A

The oxygen dissociation curve is S-shaped (sigmoidal), which is significant because it reflects the cooperative binding of oxygen to haemoglobin. This shape indicates that the binding of one oxygen molecule increases the likelihood of additional oxygen molecules binding.

123
Q

What is cooperative binding in relation to haemoglobin?

A

Cooperative binding refers to the phenomenon where the binding of one oxygen molecule to a haemoglobin subunit induces a conformational change that makes it easier for additional oxygen molecules to bind to the remaining subunits. This results in a rapid increase in saturation as pO₂ rises.

124
Q

How does the initial binding of oxygen affect haemoglobin’s affinity for subsequent oxygen molecules?

A

The initial binding of oxygen makes it more likely for subsequent oxygen molecules to bind due to the conformational change in the haemoglobin structure. This leads to a steep increase in saturation at moderate pO₂ levels on the curve.

125
Q

Why does the curve flatten at higher pO₂ levels?

A

The curve flattens at higher pO₂ levels because most of the haemoglobin binding sites are occupied, resulting in diminished incremental increases in saturation. As haemoglobin approaches full saturation, it becomes increasingly difficult for additional oxygen molecules to bind.

126
Q

What physiological advantages does cooperative binding provide?

A

Cooperative binding provides physiological advantages by allowing for efficient loading of oxygen in the lungs (where pO₂ is high) and effective unloading in tissues (where pO₂ is low). This ensures that tissues receive adequate oxygen based on their metabolic needs.

127
Q

How does carbon dioxide affect the position of the oxygen dissociation curve?

A

Carbon dioxide can shift the position of the oxygen dissociation curve to the right (the Bohr effect), indicating that at any given pO₂, haemoglobin has a lower affinity for oxygen. This promotes greater release of oxygen in metabolically active tissues where CO₂ levels are high.

128
Q

What role does pH play in influencing haemoglobin’s affinity for oxygen?

A

pH influences haemoglobin’s affinity for oxygen; lower pH (more acidic conditions) decreases affinity, promoting oxygen release. This is linked to increased CO₂ production during metabolism, which lowers pH and enhances the Bohr effect.

129
Q

How can changes in temperature affect the oxygen dissociation curve?

A

Increases in temperature can shift the oxygen dissociation curve to the right, reducing haemoglobin’s affinity for oxygen. This allows for more efficient unloading of oxygen in active tissues that generate heat during metabolism.

130
Q

Why is understanding the oxygen dissociation curve important in physiology?

A

Understanding the oxygen dissociation curve is important in physiology because it helps explain how haemoglobin functions under varying conditions, ensuring efficient transport and delivery of oxygen throughout the body, which is vital for cellular respiration and overall metabolic function.