Order of Draw and Blood Collection

Question 1

Antiseptic

Answer 1

substances used to prevent the presence of microorganisms or their toxic products within the bloodstream. Most common is 70% isopropyl. Safe for human skin

Question 2

Disinfectants

Answer 2

remove or kill microorganisms on surfaces and instruments. Examples used in lab 10% Bleach solutions. NOT safe for human skin

Question 3

Hand Sanitizer

Answer 3

an antiseptic alternative when hands are not visibly soiled.

Question 4

General Blood Collection Equipment

Answer 4

Gauze pads, bandages, slides, pen, watch, Patient ID equipment, tourniquet, Needles

Question 5

Needle Gauge

Answer 5

the bigger the gauge number the smaller the diameter and size of the needle. Most common needles are 20 to 23 gauge-needles

Question 6

Bevel

Answer 6

the slanted cut of the venipuncture needle that pierces the vein

Question 7

Shaft

Answer 7

The long cylindrical portion of the venipuncture needle

Question 8

Hub

Answer 8

the end of the venipuncture needle that attaches to the blood collection device

Question 9

Lumen

Answer 9

the internal space of the venipuncture needle

Question 10

ETS

Answer 10

Evacuated Tube System. A closed system where the patient’s blood flows through a needle inserted into a vein without contamination

Question 11

Light Blue Stopper

Answer 11

Additives: Sodium Citrate
Sample Type: Plasma
Ex Test: PT, APTT
Department: Coagulation
Invert 3-4x
Must be filled 90% for accurate readings

Question 12

Red Stopper

Answer 12

Additives: None (glass), clot activator (plastic)
Sample Type: Serum
Ex Test: Lithium, Phenytoin, Theophylline
Department: Chem, Blood Bank, Serology/Immunology
Invert 5-10x

Question 13

Green Stopper

Answer 13

Additives: Lithium heparin or Sodium heparin
Sample Type: Plasma or Whole Blood
Ex Test: Glucose, Cholesterol, Lipid Panel, Liver Panel, Chem 7
Department: Chem
Invert 5-10x

Question 14

Lavender Stopper

Answer 14

Additives: EDTA
Sample Type: Whole Blood
Ex Test: Hemoglobin, hematocrit, CBC, ESR
Department: Hematology
Invert 8-10x

Question 15

Pink Stopper

Answer 15

Additives: EDTA
Sample Type: Whole Blood
Ex Test: Blood Typing, Crossmatch
Department: Blood Bank
Invert 8-10x

Question 16

Grey Stopper

Answer 16

Additives: Sodium fluoride and Potassium oxalate
Sample Type: Whole Blood or Plasma
Ex Test: Glucose
Department: Chem
Invert 8-10x

Question 17

Orange Stopper

Answer 17

Additives: Thrombin
Sample Type: Serum
Ex Test: Glucose, Cholesterol, Lipid Panel, Liver Panel, Chem 7
Department: Chem
Invert 5-10x

Question 18

Royal Blue Stopper

Answer 18

Additives: None, EDTA, or Sodium Heparin
Sample Type: Serum, Whole Blood/Plasma, Plasma
Ex Test: Chem 7
Department: Chem
Invert 8-10x

Question 19

Yellow Stopper

Answer 19

Additives: SPS ( sodium polyanethanol sulfonate) or ACD ( acid citrate dextrose)
Sample Type: Whole Blood or Plasma
Ex Test: Blood Cultures
Department: Micro (SPS) Blood Bank/Immunohematology (ACD)
Invert 8x

Question 20

Light Green Stopper

Answer 20

Additives: Lithium heparin and gel separator
Sample Type: Plasma
Ex Test: Glucose, Cholesterol, Lipid Panel, Liver Panel, Chem 7
Department: Chem
Invert 5-10x

Question 21

Red/Black or Gold Stopper

Answer 21

Additives: Clot activator and gel separator
Sample Type: Serum
Ex Test: Chem 7
Department: Chem

Question 22

Anticoagulants

Answer 22

substances that prevent blood from clotting by precipitating calcium or inhibiting formation of thrombin
Ex: EDTA, Citrates, Heparin, Oxalates, ACD, CPD, and SPS

Question 23

Clot Activators

Answer 23

a substance that enhances coagulation in tubes.

Question 24

Order of Draw

Answer 24

1. BC
2. Sodium Citrate
3. Serum tubes with or without clot activator or gel
4. Heparin tube with or without gel
5. EDTA tube with or without gel separator
6. Sodium Fluoride/Potassium oxalate glycolytic inhibitor
   Mnemonic :
   Stop Light Red Stay Put Green LEts Go