Oncology BPG Flashcards
According to BPG, how many cultures should be set-up for an oncology sample (where the cell density allows)?
2 cultures
What is the optimum cell density for cultures?
10^6 per ml.
If not part of a clone, what is the minimum number of chromosomes a cell can contain to permit its use in the analysis?
Must have 40 or more chromosomes
Who must be involved in each case?
2 analysts must be involved and agree on the clone(s) detected, at least 1 must be a registered scientist with experience in oncology cytogenetics.
What happens if you find a single abnormal cell which is potentially clinically significant?
A single abnormal cell cannot define a clone according to BPG. However significant ones such as t(9;22) can be reported with clear qualifications.
Confirmation via FISH or molecular genetic studies should be completed if possible.
What should all reports contain?
Correct ISCN
Number of cells analysed
Description of clinically relevant abnormalities
How the abnormalities relate to the diagnosis/referral reason
Prognosis if a robust association (from trials, publications) exists.
If a clonal abnormality of unknown significance is detected how should this be reported according to BPG?
As consistent with neoplasia.
Why does the detection of -Y or -15 require special care?
May be seen in elderly patients without malignancy
According to BPG:
What turnaround time should all urgent samples meet?
What is an urgent sample?
Urgent samples; 95% should be reported in 14 calendar days.
Acute leukaemia and CML.
According to BPG: what is the turnaround time for an urgent FISH?
3 calendar days.
According to BPG what is the turnaround for a routine sample?
21 calendar days.
If monosomy 7 is detected in a myeloid disorder in a single, why do BPG state you should do?
Screen 30 cells for monosomy 7 or carry out FISH studies.
