OMIC Technologies

Question 1

Omic technologies are aimed at the detection of genes (________), mRnA (_______), protein (__________) and metabolites (_________ within in a sample

Answer 1

genomics
transcriptomics
proteomics
metabolics

Question 2

Vocab –> Libraries
Genomics: describe the mapping, sequencing and analysis of _____________
Transcriptomics: study of ____________- products transcribed
Proteomics: Analysis of all expressed _________ and interactions including all isoforms and modifications
Metabolomics: analysis of whole __________ (all ___________ synthesized)

Answer 2

all genes in genome

all RnA

Analysis of all expressed proteins

analysis of whole metabolome (all metabolites synthesized)

Question 3

Transcriptomics most used technique is DNA microarrays or known as _________
It allows real time measure of ____________ levels up to 50,000 transcripts

Used to see how ________

Limitations: requires sufficient amount of ____ and _____. Also limited data analysis bc dietary interventions usually results in ________ gene expression changes so highly reliable analysis and interpretation is required

Answer 3

CHIP
expression
nutrient effects expression levels of genes
tissue and cost
small

Question 4

Transcriptomics is used within nutrition to how a specific nutrient effects the ___________ of genes on a select group of cells. Can also be used for food safety by viewing what different genes were expressed from __________ (HWF)

Answer 4

expression
hypoallegeic wheat flour

Question 5

Proteomics involves 2D _______ ______________ and mass ____________

Used to identify protein fucntions within specific cells such as soy allergens

Answer 5

gel electrophoresis
spectromony

Question 6

metabolomics uses ________ (NMR) tech to determine the ____ and ___ of samples and the molecular structure

main challenge: metabolism is ______

NMR allows us to investigate the world at the molecular level to see the 3D structure and the way they move

Answer 6

nuclear magnetic resonance
purity and content

dynamic

Question 7

omic technologies allow for the generation of pathway information to provide capacity to measure the

Answer 7

functioning pathways of nutrients