Nucletoides Review Flashcards
What is the purpose of prpp synthetase?
Activation of ribulose 5 phosphate nucleotides (occurs before synthesis of purines)
What metabolizes are necessary for purine synthesis?
Aspartate, CO2, THFx2, glycine, glutamine
What cofactors are needed for prpp synthetase?
Mg2+ ATP
Regulation prpp synthetase
+pi, - purines
What enzyme catalyze a the rate limiting/regulated step of purine synthesis?
Glutamine:phosphoribosyl pyrophosphate amidotransferase
What regulates GPPA?
Levels of Glutamine +prpp , inhibited by amp gmp imp
What kind of crystals characterize gout?
Mono sodium urate negatively birefringent spike shaped
What precursors are needed for pyrimidine synthesis?
Glutamine, aspartic acid, and co2….NOT THF!!!!!
What is the rate limiting step of pyrimidine biosynthesis?
Cps2 yo
What is the regulation of cps 2?
+ATP, GLUTAMINE - uracil-PPP
What enzymes comprise UMP Synthase?
OMP Decarboxylase, orotate phosphoribosyl transferase
What causes orotic aciduria?
Deficiency of OMP Decarboxylase and OMP phosphoribosyl transferase
How is UTP converted to CTP!
CTP synthase Glutamine is the nitrogen donor
What enzyme catalyze a the conversion of ribonucleotides to deoxyribonucleotides?
Ribonucleotide reductase
What inhibits ribonucleotides reductase?
dATP, hydroxyurea
