Nucleic Acids Flashcards
1
Q
Building Blocks of DNA and RNA
A
5C sugar
Nitrogenous Base
Phosphate Group
2
Q
C 1
A
glycosidic bond to base
3
Q
C 2
A
Hydroxyl group in RNA
no O in DNA
4
Q
C 3
A
Hydroxyl group in DNA and RNA
joins to C5 thru phosphodiester bonds
5
Q
C 5
A
linked to phosphate group
joins C3 thru phosphodiester bonds
6
Q
AT H-bonds
A
2
7
Q
GC H-bonds
A
3
8
Q
DNA Backbone
A
Hydrophilic sugar phosphate backbone
negative charge
9
Q
High GC content
A
High Tm
10
Q
High AT content
A
Low Tm
11
Q
DNA Gyrase
A
Converts cyclic DNA to supercoiled form
12
Q
DNA Topoisomerase II
A
Converts supercoiled DNA to cyclic DNA
inhibited by quinolones
13
Q
Histones
A
rich in lysine and arginine (+ aa)
bind strongly to DNA
14
Q
Eukaryotic mRNA
A
5’ end has m7G- cap
3’ end has poly A tail
15
Q
Eukaryotic rRNA sizes
A
40S, 60S, 80S
