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BACT211 IRR1 > Nonfermenters > Flashcards

Nonfermenters Flashcards

1
Q

Grouped according to OF media and TSI reactions

A

Non fermenters

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2
Q

Nonfermenters are referred to as

A

Oxidizers or nonoxidizers

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3
Q

Enumerate the clinical infections of nonfermenter

A
  • septicemia
  • meningitis
  • osteomyelitis
  • wound infection
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4
Q

Biochemical characteristics and identification of pseudomonads

A

GAMOCCOG
- gram negative bacillus or coccobacillus
- aerobic
- motile
- oxidase positive
- catalase positive
Citrate positive
- oxidizer
- grows on MacConkey

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5
Q

What is the most commonly isolated species under pseudomonads?

A

Pseudomonas aeruginosa

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6
Q

What are the infection of pseudomonas Aeruginosa?

A

BEP UME
- bacteremia
- ecthyma gangrenosum
- pulmonary infection with cystic fibrosis

  • UTI
  • meningitis
  • endocarditis
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7
Q

What are the less serious infection of p. aeruginosa?

A
  • otitis externa
  • jacuzzi or hot tub syndrome
  • keratitis
  • endophthalmitis
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8
Q

What are the virulence factor or p. aeruginosa?

A
  • endotoxin
  • motility
  • pili
  • capsule
  • exotoxin
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9
Q

What is the most important exotoxin?

A

Exotoxin A

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10
Q

What is the pigment produced by pseudomonas fluorescence group?

A

Pyoverdine - yellow green or yellow brown pigment

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11
Q

Most strains of pseudomonas produces what pigment?

A

Pyocyanin - blue pigment

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12
Q

Enumerate the pigments produce by pseudomonas?

A
  • pyoverdine - yellow green
  • pyocyanin - blue
  • pyorubin - red
  • pyomelanin - brown or black
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13
Q

What is the medium of choice for pseudomonas aeruginosa?

A

Cetrimide agar

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14
Q

What is the odor of the colonies?

A

Sweet grape like
Corn taco like

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15
Q

P. fluorescens and P. putida do not produce what pigment?

A

Both doesn’t produce Pyocyanin

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16
Q

What are the treatment susceptible in pseudomonas?

A
  • Aminoglycosides
  • semisynthetic penicillins
  • 3rd and 4th generation cephalosporins
  • carbapenem
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17
Q

What carbohydrate does p. putida and p. fluorescens utilizes?

A

Xylose

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18
Q

What biochemical test to differentiate p. fluorescens from p. putida?

A

Gelatin hydrolysis

19
Q

P. putida and p. fluorescens susceptible to what antibiotic?

A
  • aminoglycosides
  • polymyxin
  • piperacillin
20
Q

P. putida and p. fluorescens resistant to what antibiotic?

A

Carbenicillin and SXT

21
Q

Characterized by wrinkled, leathery, adherent colonies that may produce a light yellow or brown pigment

A

Pseudomonas stutzeri

22
Q

What are the two species of acinetobacter commonly isolated from clinical specimen?

A
  • acinetobacter baumanii
  • acinetobacter lwoffi
23
Q

What acinetobacter is saccharolytic?

A

A. baumanii

24
Q

Third most common nonfermenting, gram negative bacilli, isolated from clinical specimen

A

Stenotrophomonas maltophilia

25
Q

Nonfermenter that can be mistaken as lactose fermenting colonies of MacConkey agar

A

Acinetobacter

26
Q

Nonfermentative bactefia with wrinkled colonies

A
  • pseudomonas stutzeri
  • burkholderia pseudomallei
27
Q

Enumerate the members of fluorescens group?

A
  • p. aeruginosa
  • p. putida
  • p. fluorescens
28
Q

What culture media for burkholderia pseudomallei?

A

Ashdown medium

29
Q

Enzyme being detected by the ONPG test

A

Beta-galactosidase

30
Q

A pigment unique to P. aeruginosa

A

Pyocyanin

31
Q

Culture media of choice for p. aeruginosa

A
  • cetrimide agar
32
Q

A nonfermenter that appears as purplish colonies on MacConkey media

A

Acinetobacter

33
Q

Appears bluish on MacConkey

A

Stenotrophomonas maltophilia

34
Q

Burkholderia cepacia complex typically infects

A
  • patients with chronic granulomatous disease
  • patients with cystic fibrosis
35
Q

Can grow at 4 degrees celcius

A

P. fluorescens and p. putida

36
Q

Eye infection is associated with

A

A. baumanii

37
Q

What acinetobacter is asaccharolytic

A

Acinetobacter lwoffi

38
Q

Acinetobacter baumanii is resistant to

A

Penicillins, 1st and 2nd generation cephalosporins

39
Q

Found to contaminated blood drawing equipment and disinfectants

A

Stenotrophomonas maltophilia

40
Q

Usually associated with pneumonia in patients with cystic fibrosis or chronic granulomatous disease

A

Burkholderia cepacia complex

41
Q

Causes glanders disease

A

Burkholderia mallei

42
Q

Causes melioidosis, a pulmonary disease. The organism found in water and muddy soils.

A

Burkholderia pseudomallei

43
Q

It is nonfermentative, wrinkled colony that has safety pin appearance

A

Burkholderia pseudomallei

44
Q

It appears deep pink in ashdown medium and exhibits an earthy odor

A

Burkholderia pseudomallei

BACT211 IRR1 (6 decks)

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