Nonfermenters Flashcards
Grouped according to OF media and TSI reactions
Non fermenters
Nonfermenters are referred to as
Oxidizers or nonoxidizers
Enumerate the clinical infections of nonfermenter
- septicemia
- meningitis
- osteomyelitis
- wound infection
Biochemical characteristics and identification of pseudomonads
GAMOCCOG
- gram negative bacillus or coccobacillus
- aerobic
- motile
- oxidase positive
- catalase positive
Citrate positive
- oxidizer
- grows on MacConkey
What is the most commonly isolated species under pseudomonads?
Pseudomonas aeruginosa
What are the infection of pseudomonas Aeruginosa?
BEP UME
- bacteremia
- ecthyma gangrenosum
- pulmonary infection with cystic fibrosis
- UTI
- meningitis
- endocarditis
What are the less serious infection of p. aeruginosa?
- otitis externa
- jacuzzi or hot tub syndrome
- keratitis
- endophthalmitis
What are the virulence factor or p. aeruginosa?
- endotoxin
- motility
- pili
- capsule
- exotoxin
What is the most important exotoxin?
Exotoxin A
What is the pigment produced by pseudomonas fluorescence group?
Pyoverdine - yellow green or yellow brown pigment
Most strains of pseudomonas produces what pigment?
Pyocyanin - blue pigment
Enumerate the pigments produce by pseudomonas?
- pyoverdine - yellow green
- pyocyanin - blue
- pyorubin - red
- pyomelanin - brown or black
What is the medium of choice for pseudomonas aeruginosa?
Cetrimide agar
What is the odor of the colonies?
Sweet grape like
Corn taco like
P. fluorescens and P. putida do not produce what pigment?
Both doesn’t produce Pyocyanin
What are the treatment susceptible in pseudomonas?
- Aminoglycosides
- semisynthetic penicillins
- 3rd and 4th generation cephalosporins
- carbapenem
What carbohydrate does p. putida and p. fluorescens utilizes?
Xylose
What biochemical test to differentiate p. fluorescens from p. putida?
Gelatin hydrolysis
P. putida and p. fluorescens susceptible to what antibiotic?
- aminoglycosides
- polymyxin
- piperacillin
P. putida and p. fluorescens resistant to what antibiotic?
Carbenicillin and SXT
Characterized by wrinkled, leathery, adherent colonies that may produce a light yellow or brown pigment
Pseudomonas stutzeri
What are the two species of acinetobacter commonly isolated from clinical specimen?
- acinetobacter baumanii
- acinetobacter lwoffi
What acinetobacter is saccharolytic?
A. baumanii
Third most common nonfermenting, gram negative bacilli, isolated from clinical specimen
Stenotrophomonas maltophilia
Nonfermenter that can be mistaken as lactose fermenting colonies of MacConkey agar
Acinetobacter
Nonfermentative bactefia with wrinkled colonies
- pseudomonas stutzeri
- burkholderia pseudomallei
Enumerate the members of fluorescens group?
- p. aeruginosa
- p. putida
- p. fluorescens
What culture media for burkholderia pseudomallei?
Ashdown medium
Enzyme being detected by the ONPG test
Beta-galactosidase
A pigment unique to P. aeruginosa
Pyocyanin
Culture media of choice for p. aeruginosa
- cetrimide agar
A nonfermenter that appears as purplish colonies on MacConkey media
Acinetobacter
Appears bluish on MacConkey
Stenotrophomonas maltophilia
Burkholderia cepacia complex typically infects
- patients with chronic granulomatous disease
- patients with cystic fibrosis
Can grow at 4 degrees celcius
P. fluorescens and p. putida
Eye infection is associated with
A. baumanii
What acinetobacter is asaccharolytic
Acinetobacter lwoffi
Acinetobacter baumanii is resistant to
Penicillins, 1st and 2nd generation cephalosporins
Found to contaminated blood drawing equipment and disinfectants
Stenotrophomonas maltophilia
Usually associated with pneumonia in patients with cystic fibrosis or chronic granulomatous disease
Burkholderia cepacia complex
Causes glanders disease
Burkholderia mallei
Causes melioidosis, a pulmonary disease. The organism found in water and muddy soils.
Burkholderia pseudomallei
It is nonfermentative, wrinkled colony that has safety pin appearance
Burkholderia pseudomallei
It appears deep pink in ashdown medium and exhibits an earthy odor
Burkholderia pseudomallei
