Nonfermenters

Question 1

Grouped according to OF media and TSI reactions

Answer 1

Non fermenters

Question 2

Nonfermenters are referred to as

Answer 2

Oxidizers or nonoxidizers

Question 3

Enumerate the clinical infections of nonfermenter

Answer 3

• septicemia
• meningitis
• osteomyelitis
• wound infection

Question 4

Biochemical characteristics and identification of pseudomonads

Answer 4

GAMOCCOG
- gram negative bacillus or coccobacillus
- aerobic
- motile
- oxidase positive
- catalase positive
Citrate positive
- oxidizer
- grows on MacConkey

Question 5

What is the most commonly isolated species under pseudomonads?

Answer 5

Pseudomonas aeruginosa

Question 6

What are the infection of pseudomonas Aeruginosa?

Answer 6

BEP UME
- bacteremia
- ecthyma gangrenosum
- pulmonary infection with cystic fibrosis

• UTI
• meningitis
• endocarditis

Question 7

What are the less serious infection of p. aeruginosa?

Answer 7

• otitis externa
• jacuzzi or hot tub syndrome
• keratitis
• endophthalmitis

Question 8

What are the virulence factor or p. aeruginosa?

Answer 8

• endotoxin
• motility
• pili
• capsule
• exotoxin

Question 9

What is the most important exotoxin?

Answer 9

Exotoxin A

Question 10

What is the pigment produced by pseudomonas fluorescence group?

Answer 10

Pyoverdine - yellow green or yellow brown pigment

Question 11

Most strains of pseudomonas produces what pigment?

Answer 11

Pyocyanin - blue pigment

Question 12

Enumerate the pigments produce by pseudomonas?

Answer 12

• pyoverdine - yellow green
• pyocyanin - blue
• pyorubin - red
• pyomelanin - brown or black

Question 13

What is the medium of choice for pseudomonas aeruginosa?

Answer 13

Cetrimide agar

Question 14

What is the odor of the colonies?

Answer 14

Sweet grape like
Corn taco like

Question 15

P. fluorescens and P. putida do not produce what pigment?

Answer 15

Both doesn’t produce Pyocyanin

Question 16

What are the treatment susceptible in pseudomonas?

Answer 16

• Aminoglycosides
• semisynthetic penicillins
• 3rd and 4th generation cephalosporins
• carbapenem

Question 17

What carbohydrate does p. putida and p. fluorescens utilizes?

Answer 17

Xylose

Question 18

What biochemical test to differentiate p. fluorescens from p. putida?

Answer 18

Gelatin hydrolysis

Question 19

P. putida and p. fluorescens susceptible to what antibiotic?

Answer 19

• aminoglycosides
• polymyxin
• piperacillin

Question 20

P. putida and p. fluorescens resistant to what antibiotic?

Answer 20

Carbenicillin and SXT

Question 21

Characterized by wrinkled, leathery, adherent colonies that may produce a light yellow or brown pigment

Answer 21

Pseudomonas stutzeri

Question 22

What are the two species of acinetobacter commonly isolated from clinical specimen?

Answer 22

• acinetobacter baumanii
• acinetobacter lwoffi

Question 23

What acinetobacter is saccharolytic?

Answer 23

A. baumanii

Question 24

Third most common nonfermenting, gram negative bacilli, isolated from clinical specimen

Answer 24

Stenotrophomonas maltophilia

Question 25

Nonfermenter that can be mistaken as lactose fermenting colonies of MacConkey agar

Answer 25

Acinetobacter

Question 26

Nonfermentative bactefia with wrinkled colonies

Answer 26

- pseudomonas stutzeri - burkholderia pseudomallei

Question 27

Enumerate the members of fluorescens group?

Answer 27

- p. aeruginosa - p. putida - p. fluorescens

Question 28

What culture media for burkholderia pseudomallei?

Answer 28

Ashdown medium

Question 29

Enzyme being detected by the ONPG test

Answer 29

Beta-galactosidase

Question 30

A pigment unique to P. aeruginosa

Answer 30

Pyocyanin

Question 31

Culture media of choice for p. aeruginosa

Answer 31

- cetrimide agar

Question 32

A nonfermenter that appears as purplish colonies on MacConkey media

Answer 32

Acinetobacter

Question 33

Appears bluish on MacConkey

Answer 33

Stenotrophomonas maltophilia

Question 34

Burkholderia cepacia complex typically infects

Answer 34

- patients with chronic granulomatous disease - patients with cystic fibrosis

Question 35

Can grow at 4 degrees celcius

Answer 35

P. fluorescens and p. putida

Question 36

Eye infection is associated with

Answer 36

A. baumanii

Question 37

What acinetobacter is asaccharolytic

Answer 37

Acinetobacter lwoffi

Question 38

Acinetobacter baumanii is resistant to

Answer 38

Penicillins, 1st and 2nd generation cephalosporins

Question 39

Found to contaminated blood drawing equipment and disinfectants

Answer 39

Stenotrophomonas maltophilia

Question 40

Usually associated with pneumonia in patients with cystic fibrosis or chronic granulomatous disease

Answer 40

Burkholderia cepacia complex

Question 41

Causes glanders disease

Answer 41

Burkholderia mallei

Question 42

Causes melioidosis, a pulmonary disease. The organism found in water and muddy soils.

Answer 42

Burkholderia pseudomallei

Question 43

It is nonfermentative, wrinkled colony that has safety pin appearance

Answer 43

Burkholderia pseudomallei

Question 44

It appears deep pink in ashdown medium and exhibits an earthy odor

Answer 44

Burkholderia pseudomallei