Fastidious Flashcards

1
Q

Require growth factors

A

X and v factor

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2
Q

Non-motile, facultatively anaerobic, oxidase and catalase positive, nitrate reductase positive

A

Haemophilus

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3
Q

Phenomenon observed in species requiring V factor

A

Satellitism

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4
Q

Erroneously implicated in the influenza pandemic of 1889-1890

A

Haemophilus influenzae

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5
Q

What are the virulence factors of Haemophilus influenzae?

A
  • capsule
  • IgA protease
  • adherence mechanism
  • outer membrane proteins
  • lipopolysaccharide
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6
Q

Causes osteoarthritis in children

A

Kingella kingae

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7
Q

Bleach like odor

A

Haemophilus influenzae

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8
Q

Legionella requires ______ for growth

A

L-cysteine

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9
Q

Mushroom odor

A

Haemophilus influenzae

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10
Q

Pinkeye conjunctivitis

A

Haemophilus aegypticus

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11
Q

Urine specimen for Ag detection

A

Legionella

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12
Q

What are the two patterns of H. influenzae?

A
  • Encapsulated strains
  • unencaosulated strains
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13
Q

Haemophilus influenzae serotype b (HIB) in children

A

Meningitis

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14
Q

Rapid onset, acute inflammation, intense edema of the epiglottis

A

Epiglottitis

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15
Q

Life threatening disease in children

A

Bacterial tracheitis

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16
Q

What is other name of harmophilus aegypticus?

A

Koch-Weeks bacillus

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17
Q

Observed in conjunctivitis exudates from egyptians by koch in 1883

A

Haemophilus aegypticus

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18
Q

Associated with pinkeye conjunctivitis

A

Haemophilus aegypticus

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19
Q

Agent of chancroid, type of genital ulcer disease

A

Haemophilus ducreyi

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20
Q

Infects mucosal epithelium, genital and nongenital skin, and regional lymph nodes

A

Haemophilus ducreyi

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21
Q

Endocarditis of insidious onset

A

Haemophilus parainfluenzae

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22
Q

Pharyngitis

A

Haemophilus parahaemolyticus

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23
Q

What are the specimen for Haemophilus?

A
  • Blood
  • CSF
  • middle ear exudate
  • joint fluids
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24
Q

What we can use for response specimens

A

CAP supplemented with bacitracin (300 mg/L)

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25
Q

For H. aegypticus and H. ducreyi what media can we use ?

A

CAP supplemented with 1% Iso-VitaleX or Vitox

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26
Q

Translucent, tannish, moist, smooth, convex, with distinct odor

A

Haemophilus influenzae

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27
Q

Small, flat, smooth, nonmucoid, transparent to opaque, tan or yellow

A

Haemophilus ducreyi

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28
Q

School of fish or railroad tracks appearance

A

Haemophilus ducreyi

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29
Q

Used to differentiate heme-producing species of Haemophilus

A

Porphyrin test

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30
Q

Test the ability of the organisms to convert d-aminolevulinic acid (d-ALA) into porphyrins or porphobilinogens

A

Porphyrin test

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31
Q

Cannot synthesize heme and are not X factor positive (required hemin)

A

Porphyrin negative

32
Q

Can synthesize heme and x factor negative

A

Porphyrin positive

33
Q

What reagent is addrd to porphobilinogen?

A

Kovac’s reagent

34
Q

HACEK

A

H - Haemophilus
A - aggregatibacter, actinomycetemcomitans, A. aphrophilus
C - cardiobacterium hominis
E - eikenella corrodens
K - kingella spp

35
Q

Found in dental plaque and gingival scrapings

Colonies are conves, granular, and yellow with an opaque zone near the center of CAP

A

Aggregatibacter aphrophilus

36
Q

Isolated from blood, lung tissue, abscessess of the mouth and brain

Isolated grow better in increased carbon dioxide

A

Aggregatibacter actinomycetemcomitans

37
Q

Form rosettes, swellings, long filaments or sticklike structures in yeast extract

A

Cardiobacterium hominis

38
Q

Agar pitting colonies

A

Cardiobacterium hominis

39
Q

Cellulitis in drug addict

Implicated in periodontitis, meningitis, empyema, pneumonia, osteomyelitis, arthritis

A

Eikenella corrodens

40
Q

Members are coccobacillary to short bacilli with swuared ends that occur in paird or short chains

Grows on Thayer-Martin agar

A

Kingella

41
Q

Has been associated with bacteremia and abscesses

A

Kingella dinitrificans

42
Q

Important pathogrn in pediatric population

A

Kingella kingae

43
Q

Systemic and pneumonic forms are possible but cutaneous infection resulting from animals bites is common

A

Pasteurella

44
Q

What is the most common specie of pasteurella?

A

Pasteurella multocida

45
Q

Also known as mediterranean, crimean, malta fevers

A

Brucella

46
Q

Nonspecific symptoms occur within 1-4 weeks of exposure

A

Acute infection

47
Q

Undulating fevers, arthritis and epididymoorchitis

A

Subchronic or undulant form

48
Q

Depression, arthritis, and chronic fatigue syndrome

A

Chronic form

49
Q

Four common isolated species of brucella

A

Brucella melitensis
Brucrlla abortus
Brucella suis
Brucella canis

50
Q

B. abortus natural host

A

Cattle

51
Q

Brucella suis natural host

A

Swine

52
Q

B. melitensis natural host

A

Goat or sheep

53
Q

B. canis natural host

A

Dogs

54
Q

Most common specie of francisella

A

Francisella tularensis

55
Q

Zoomotic disease also known as rabbit fever, deerfly fever, lemming fever, water rat trappers’ disease

A

Tularemia

56
Q

Associated with nosocomial infection.

A

Legionella

57
Q

Most common specie of legionella

A

Legionella pneumophila

58
Q

Found in lakes, rivers, hot springs, and mud

A

Legionella

59
Q

Media used for isolation of legionella

A

Buffered charcoal yeast extract agar BCYE with L-cysteine

60
Q

On BCYE the colonies appear

A

Grayish white, blue-green, convex, glistening.

61
Q

Ground glass appearance

A

Legionella

62
Q

What are the rapid methods?

A
  • urine antigen test
  • direct fluorescent antibody rest
63
Q

Detection of legionella antigen in urine specimen using radioimmunoassay and enzyme immunoassay

A

Urine antigen test

64
Q

Fluorescein isothiocyanate labelled antibodies bind to antigens on the cell surface, and the Ag-Ab complexexls are detected using fluorescent microscope

A

Direct fluorescent antibody test

65
Q

Primary human pathogens

A

Bordetella pertussis and borderella parapertussis

66
Q

Causes whooping cough or pertussis

A

Bordetella

67
Q

Opportunistic pathogen

A

Bordetella bronchiseptica

68
Q

What are the virulence factors of bordatella

A
  • filamentous hemagglutinin
  • pertactin
  • pertussis toxin
  • tracheal cytotoxin
69
Q

Nonspecific signs; infection is highly communicable

A

Catarrhal phase

70
Q

Sudden onset of severe, repetitive coughing followed by fever ye characteristics “whoop” at the end of coughing spell

A

Paroxysmal phase

71
Q

Decrease in frequency and severity of coughing spells

A

Convalescent phase

72
Q

Human disease with no animal reservoir or vector

Acquired through respiratory droplet or direct contact with secretion

A

Pertussis

73
Q

One of the most highly communicable diseases of childhood

A

Pertussis

74
Q

Respiratory tract pathogen of animals includding dogs, in which it causes kennel cough

A

Bordetella bronchiseptica

75
Q

Specimen of choice for brucella

A

Nasopharyngeal aspirates or swabs