NON ENTERIC GI PATHOGEN Flashcards
curved/comma bacillus
found in brackish, marine water, salt water
halophilic
vibrio
vibrio are all NLF except for
v. vulnificus
CA cholera, necrotizing faciitis
v. cholerae O1
all vibrio reduce nitrates to nitrites except for`
v. metschnikovii
v. cholerae 01 serotypes
A,B
ogawa
v. cholerae 01 serotypes
A,C
inaba
v. cholerae 01 serotypes
A,B,C
hikojima
v. cholerae biogroups
VP (-), do not agglutinate chicken RBC, susceptible
classical
v. cholerae biogroups
VP (+), agglutinates chicken RBC, resistant
el tor
enterotoxins:
cho9lera toxins
zot toxins
ace toxins
v. cholerae
+ string test
mucoid stringing reaction
v. cholerae
antigenic structure: somatic O, flagellar H
v. cholerae
acute diarrheal disease spread through contaminated water
hallmark: rice-watery stool
cholera
protein with enzymatic A subunit and intestinal receptor-binding B subunit
stimulate hypersecretion of water and chloride
choleragen
second most common vibrio implicated in gastroenteritis
SUMMER DIARRHEA in japan
v. parahemolyticus
pandemic strain; food borne outbreaks
v. parahemolyticus serotype O3:K6
hemolysis lyses human RBCs
kanagawa phenomenon
selective medium for v. parahemolyticus –
high salt mannitol medium
wagatsuma agar
lactose-positive vibrio
second to v. cholerae in producing serious type of vibrio
v. vulnificus
from eating raw oysters, fish and TILAPIA
v. vulnificus
least pathogenic vibrio- strictly halophile 1-10% NaCl
OCCUPATIONAL HAZARD fishermen and sailors
v. alginolyticus
vibrio species should be collected and transported only to – medium
cary-blair
yellow colonies on TCBS: CAM- v. cholerae, v. alginolyticus, v. metschnikovii
sucrose fermenters
green colonies on TCBS:
non-sucrose fermenters
USES 0.5% nA DEOXYCHOLATE
string test
differentiates vibrio from aeromonas
+= vibrio
string test
used to separate vibrios (SUSCEPTIBLE) from aeromonas (RESISTANT)
vibriostatic test
differentiate v. cholerae 01 and non-O1 from other vibrio
vibriostatic test
best method for vibrio spp
API 20E
strains that resembles v.cholerae but fail to agglutinate in O1 antisera are reffered
v. cholerae non-01
antigenic structure: O and K antigens
v. parahemolyticus
fresh water, chlorinated water
BULLS EYE COLONIES, APRON-like patterns
aeromonas
CA RED LEG diseases in frogs
CA traveler’s diarrhea similar to ETEC
aeromonas
group of aermonas
a.salmonicida (fish pathogen)
psychrophilic group
most common isolate (aeromonas)
a. caviae
CAMPYLOBACTER requires 5-10% oxygen (microaerophilic) except for – ANAEROBE
c. rectus, c. carvus
CA of guillan-barre syndrome
causing sterility and abortion
campylobacter
SEAGULL-wing shaped
tailling effect along the streak line
runny spreading
campylobacter
most common cause of bacterial gastroenteritis worldwide from contaminated chicken and turkey
c. jejuni
causes septic arthritis
DARTING MOTILITY
c. jejuni
recommended counterstain for campylobacter
carbolfuchsin
used in direct detection of c. jejuni and c. coli
EIA
S-shaped rods resembling campylobacter
STRONG UREASE ACTIVITY
helicobacter
major cause of type B gastritis, peptic ulcer, gastric carcinoma
h. pylori
neutralizes gastric acid
urease
isolated from blood of patients with bacteremia and patients with HIV
h. cinaedi
medium used for tissue biopsy material: helicobacter
stuarts
presence of - stimulates further growth
hemin
excellent sensitivity and specificity in helicobacter
urea breath test, PCR
Enteric pathogens
YESS
yersinia, salmonella, shigella, e.coli O157:H7
PVAC
plesiomonas shigelloides, vibrio, aeromonas, campylobacter