I. ENTEROBACTERIACEAE Flashcards by JHOANNA LYNN LUMBOY

gram negative
non spore
f. anaerobic bacilli
reduces nitrate to nitrite
ferments glucose

present in intestinal tract as commensal flora

enterobacteriaceae

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enterobacteriaceae are usually present as commensal flora except for

OVERT OR TRUE PATHOGENS

yersinia, shigella, salmonella

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enterobacteriaceae are large smooth gray colonies, non encapsulated except

klebsiella, enterobacter

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enterobacteriaceae are non hemolytic except for –

E. coli (b-hemolytic)

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all enterobacteriaceae are motile with peritrichous flagella except –

klebsiella, shigella, yersinia

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may grow at low temp 1-5c

serratia, yersinia

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extended spectrum b lactamase producing enterobacteria

EKK, e.coli, k. pneu, k. oxy

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isolated from urinary tract and cause bacteremia

EPK, e. coli, p.mirabilis, k. pneu

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antibiotic resistant genera

SEC, serratia, enterobacter, citro

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assoc with diarrhea

yersinia, e.coli, shigella, salmonella

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antigen determinants

heat stable; cell wall for e.coli and shigella

somatic o

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antigen determinants

heat labile; flagellum for salmonella

flagellar h

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antigen determinants

heat labile; covers o antigen

found as k1- e. coli
found as Vi- s. enterica

capsular k

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infection:

neonatal sepsis

e. coli

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infection:

dysentery

shigella

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infection:

plague

y. pestis

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infection:

wounds contaminated with soil and vegatation

erwinia

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infection:

meningitis

e.coli, edwardsiella

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infection:

enteric fever

edwardsiella, salmonella

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infection:

mesenteric adenitis

y. pseudotuberculosis, entero

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colon bacillus
inhabits normal bowel flora and female genital tract
invades enterocytes lining of the large intestine

marker of fecal contamination in water purification
leading cause of nosocomial infection- UTI

e. coli

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flat dry with pink colonies: mc conkey
greenish metallic sheen: EMB

e. coli

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antigenic determinants: O,H,K
VF: endotoxin, common pilli, intimin, k1 antigen. sex pili and adhesive fimbriae

e.coli

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VF that causes neonatal meningitis in e. coli

k1 antigen

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formerly called as e.coli atypical or enteric group 2 isolated from CSF, wounds and blood culture: yellow-pigmented colonies

e. hermanii

infection: infantile diarrhea stool wo blood

enteropathogenic E. coli EPEC

infection: traveler's diarrhea BAP

`enterotoxigenic E. coli ETEC

infection: montezumas's revenge BAP

`enterotoxigenic E. coli ETEC

infection: dysentery like-shigella like infx watery diarrhea with WBCs

enterinvasive E.coli EIEC

infection: hemorrhagic colitis hemolytic uremic syndrome

enterohemorrhagic e.coli EHEC

infection: watery diarrhea

enteroadherent e.coli EAEC

infection: UTI

enteroadherent e.coli EAEC diffusely adherent e.coli DAEC

adheres to Hep2 cells forming clumps of bacteria 'stacked brick appearance'

enteroadherent e.coli EAEC

most common cause of UTIs in human

uropathogenic e.coli

determines the organim's ability to produce keratoconjuctivitis in guinea pig, virulence test for shigella and EIEC

sereny test

most common cause of UTIs in human

uropathogenic e.coli

production of cGMP

production cAMP

phage encoded cytotoxin identical to the shiga toxon produced by s.dysenteriae produces damage to vero cells (african green monkey)

veratoxin I

e.coli strains that produce verotoxins are also called as -

STEC shiga toxigenic e.coli

friedlander's bacillus bacillus capsulatus

klebsiella pneu subsp pneu

CA of community acquired pneumonia currant jelly like sputum LOWER respiratory tract infx

k. pneu subsp pneu

CA of chronic atrophic rhinitis foul smelling rhinitis

k.pneu subsp ozanae

CA of granuloma of nose and osopharynx

k.pneu subsp rhicoscleromatis

all + except for indole, gelatin

k pneu pneu

all - except for lysine

k pneu oza

all + except for gelatin

k pneu oxy

resembles klebsiella when gwoing on mac conkey

enterobacter

formerly known as e. taylorae isolated with osteomyelitis following traumatic wounds

enterobacter cancerogenus

formerly known as enterobacter agglomerans from POWDERED INFANT formula

chronobacter sakazakil

formely known as enterobacter agglomerans from CONTAMINATED IV FLUIDS triple decarboxylase negative

pantoea agglomerans

serratia musty pungent odor potato like

s. odorifera

most clinically significant in serratia species bacteremic outbreaks in nurseries, burn units, cardiac surgery epidemic of SEPTIC ARTHRITIS

s. marcescens

causes osteomyelitis following a motorcycle accident

s. plymuthica

has a delayed citrate reaction

hafnia

hafnia that grows in beer wort of breweries

h. alvei biotype 1

can cause acute glomerular nephritis a rapid urease producer=renal stone formation

proteus most common isol: p. mirabilis

swarming phenomenon burnt chocolate/burnt gun powder odor

proteus

nosocomial outbreaks in burn units

providencia stuartil

same biochemical reaction with P. vulgaris except citrate -

morganella

isolated from cold blooded and warm blooded animals

edwardsiella

plant pathogen

erwinia

resmbles e.coli on mac conkey biochemically resembling salmonella

citrobacter

causes false + agglutination test with salmonella

citrobacter

endocarditis with IV drug users

citrobacter freundii

produces type 1 cephalosporinase

citrobacter freundii

nursery outbreak of neonatal meningitis

citrobacter koseri

formerly known as c.diversus

c. koseri

most serious pathogenic enterobacteria causing enteric fever, typhoid, gastroenteritis (food poisoning) can be carried by human carriers found in cold blooded animals as well as rodents and birds: natural hosts

salmonella

VF: fimbriae and enterotoxin somatic o and flagellar H antigen, Vi antigen

salmonella

CA of enteric fever

S. serotype typhi

CA of parathypoid fever

s. serotype paratyphi a,b,c s serotype choleraesuis

salmonella all are motile except

s. pullorum, s. gallinarum

salmonella all produces gas except for

s. gallinarum, s. typhi

salmonella found in lizards and other cold blooded animals

s. bongori

most common form of food poisoning mostly caused by s. enterica subsp enterica

gastroenteritis

CA peanut butter outbreaks

s. typhimurium

also known as typhoid fever caused by s. typhi MOT: transmission thru fomites is possible, ingestion of contaminated food and water, human carriers

enteric fever

characteristics: ROSE SPOTS- 2nd week of fever complications: stays/harbor GALLBLADDER causing necrosis

enteric fever

necrotizing cholecystitis PEYER's patches

enteric fever

CA of enteric fever resistant to gastric acids

S. typhi

specimen for salmonella: 1st week of infx

blood

specimen for salmonella: 2nd week of infx

stool

specimen for salmonella: 3rd week of infx

urine

intracellular organism multiply within the cells of colon transmitted by 4Fs flies, fingers, food, feces somatic O antigen spx: rectal swab

shigella

most virulent shigella

s. dysenteriae

CA of gay bowel syndrome

shigella flexneri

CA of bacillary dysentery

s. dysenteriae type 1

acute inflammatory colitis, bloody diarrhea (WBCs in tool) caused: improper sanitary conditions-poor personal hygiene highly communicable symptoms: tenesmus complications: ileus-obstruction of the intestine

bacillary dysentery

plague bacillus class a bioterrorism agent CA of bubonic plague/ black death

yersinia pestis

MOT: bite of xenopsylla cheopis has the ability to survive and multiply inside phagocytes

yersinia pestis

closed safety pin appearance stalactile pattern

y. pestis

plague: transmitted by fleas/rats/rodents which multiply in BLOOD and LYMPHS -- inflammatory swelling of axilla and groin buboes results from infected flea

bubonic plague

plague: transmitted by fleas/rats/rodents which multiply in BLOOD and LYMPHS -- acquired by close contact with victims, secondary to bubonic plague

pulmonary plague

CA of enterocolitis survive in cold temperature (food refrigeration) contaminated packed RBCs requires cold enrichment technique BULLS EYE COLONIES

yersinia enterocolitoca

only oxidase + member of the enterobacteriaceae

plesiomonas shiggeloides

found in freshawater- warmer climates APRON like colonies ingestion of uncooked oysters, dlamps, shrimps

plesiomonas shigelloides