NON-BRANCHING, SPORE-FORMING, Catalase-POSITIVE, Gram-positive Bacilli Flashcards

Bacillus and similar organisms

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1
Q

colony appearance of Bacillus anthracis

A

Medusa Head
ground glass colonies

in 5% BAM

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2
Q

three forms of diseases causeed by B. anthracis

A

cutaneous
gastrointestinal
pulmonary (Wool sorter’s disease/Rag Picker’s disease)

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3
Q

a black necrotic lesionin cutaneous anthracis

A

Eschar

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4
Q

the composition of the anthrax toxin consists of the following proteins:

A

Protective Antigen (PA) – facilitates the transport of the 2 other proteins ino the cell
Edema Factor
Lethal factor

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5
Q

laboratory identification of Bacillus cereus

A
  1. penicillin resistant
  2. B-hemoolytic
  3. motile
  4. wide zone of lethicinase on Egg Yolk Agar
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6
Q

the two tyes of symptoms upon the ingestion of B. cereus from contaminated food

A
  1. diarrhea and abdominal pain – 8 to 16 hours

2. nausea and vomitting – 1 to 5 hours

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7
Q

toxins produced in diarrheal B. cereus infection

A
  • hemolysin BL (hBL)
  • nonhemolytic enterotoxin (Nhe)
  • cytotoxin K (CytK)
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8
Q

toxin in diarrheal B. cereus infection responsible for its major symptoms

A

Nhe

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9
Q

the emetic form of illness is associated to this toxin

A

cereulide (heat stable, proteolytic, acid resistant)

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10
Q

bacilli that is found in feces without the presence of gastrointestinal disturbance

A

Bacillus thuringiensis

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11
Q

method od specimen collection in cutaneous anthrax

A

collection of specimen beneath the eschar

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12
Q

inhalattion anthrax specimens

A

Blood culture speecimen
Pleural fluid
a specimen for serology

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13
Q

an enrichment or selective technique used in order to eliminate conttaminants and enhance the growth of Bacillus

A

Alcohol or heat shock

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14
Q

the only specific procedure to directly detect Bacillus anthracis

A

Gram Stain

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15
Q

sporulation of Baacillus is enhanced by the following:

A

oxygen
triple iron sugar
urea
nutrient agar with 5 mg/L manganese sulfate

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16
Q

spore stain components/method

A
  1. smear covered with primary stain malachite green and filter paper
  2. heated but the slide should be kept moist
  3. safraanin counterstain
17
Q

all Bacillus grow in the following culture media

A

BAM
CAM

selecttive media:
CNA
PEA
PLET (polymyxin-lysosome-EDTA-thallous acetate)

18
Q

heat shock treatment utilized for sporulation is achieved at these temperatures for most Bacillus spp exceept B. anthracis

A

70C for 30 minutes ;
80C for 10 minutes

effeective in eliminating vegeetative organisms

19
Q

heat shock treatment to enduce sporulation in B. anthracis

A

62 to 65C 15 to 20 mins