NON-BRANCHING, SPORE-FORMING, Catalase-POSITIVE, Gram-positive Bacilli Flashcards
Bacillus and similar organisms
colony appearance of Bacillus anthracis
Medusa Head
ground glass colonies
in 5% BAM
three forms of diseases causeed by B. anthracis
cutaneous
gastrointestinal
pulmonary (Wool sorter’s disease/Rag Picker’s disease)
a black necrotic lesionin cutaneous anthracis
Eschar
the composition of the anthrax toxin consists of the following proteins:
Protective Antigen (PA) – facilitates the transport of the 2 other proteins ino the cell
Edema Factor
Lethal factor
laboratory identification of Bacillus cereus
- penicillin resistant
- B-hemoolytic
- motile
- wide zone of lethicinase on Egg Yolk Agar
the two tyes of symptoms upon the ingestion of B. cereus from contaminated food
- diarrhea and abdominal pain – 8 to 16 hours
2. nausea and vomitting – 1 to 5 hours
toxins produced in diarrheal B. cereus infection
- hemolysin BL (hBL)
- nonhemolytic enterotoxin (Nhe)
- cytotoxin K (CytK)
toxin in diarrheal B. cereus infection responsible for its major symptoms
Nhe
the emetic form of illness is associated to this toxin
cereulide (heat stable, proteolytic, acid resistant)
bacilli that is found in feces without the presence of gastrointestinal disturbance
Bacillus thuringiensis
method od specimen collection in cutaneous anthrax
collection of specimen beneath the eschar
inhalattion anthrax specimens
Blood culture speecimen
Pleural fluid
a specimen for serology
an enrichment or selective technique used in order to eliminate conttaminants and enhance the growth of Bacillus
Alcohol or heat shock
the only specific procedure to directly detect Bacillus anthracis
Gram Stain
sporulation of Baacillus is enhanced by the following:
oxygen
triple iron sugar
urea
nutrient agar with 5 mg/L manganese sulfate