Haemophilus spp.

Question 1

Haemophilus spp require these in in vitro growth

Answer 1

X factor (Hemin)
V factor (NAD/NADP)

Question 2

Haemophilus spp normally inhabit the upper respiratory tract except for:

Answer 2

Haemophilus ducreyi

Question 3

composition of the capsule of H. influenzae

Answer 3

sugar-alcohol phosphate aka polyribitol phosphate

Question 4

most commonly encountered type of H. influenzae

Answer 4

H. influenzae type b

Question 5

Haemophilus ducreyi is commonly isolated in the:

Answer 5

genital area

– genital lesions

Question 6

specimen collection indicated for H. ducreyi

Answer 6

cotton swab moistened with phosphate buffered saline

– collected at th ebase of the ulcer

Question 7

indications for centrifugation in order to optimize or increase the sensitivity of direct detection of Haemophilus in Gram stain

Answer 7

centrifuge at 2000 rpm for 10 minutes

– cytocentrifuge at 10,000 rpm for 10 minutes

Question 8

appearance of H. ducreyi in Gram Stain

Answer 8

SCHOOL OF FISH APPEARANCE

Question 9

the culture media that contains both that X factor (hemin) and V factor (NADP)

Answer 9

chocolate agar

Question 10

isolation of H. influenzae may be achieved thru inoculation in

Answer 10

horse blood bacitracin agar

– from patients with cystic fibrosis; prevents the growth of Pseudomonas aeruginosa

Question 11

number of days to incubate H. ducreyi

Answer 11

7 days