NON-BLOOD SPECIMEN HANDLING AND PROCESSING Flashcards
BODY FLUIDS
Cerebrospinal Fluid (CSF)
Peritoneal Fluid
Pericardial Fluid
Pleural Fluid
NON-BLOOD SAMPLES
BODY FLUIDS
URINE
STOOL
SPUTUM
SEMEN
OROPHARYNGEAL (OP)
NASOPHARYNGEAL (NP) SWAB NASOPHARYNGEAL WASH/ASPIRATE
TISSUES
is the most common sample the patient needs to collect.
Urine
Urine: for samples that will be used for culture, the container must be
sterile
Urine: Collection by a _____ is the most common method.
patient
is used to collect urine for younger children bec they do not have control of their bladder function.
Pediatric urine collection bag
URINE TYPE:
Random
first morning
8-hour sample
timed sample
double voided
24-hour sample
URINE COLLECTION:
Mid-stream
clean-catch
catheter (culture)
URINE VOLUME
30 - 60ml
URINE PRESERVATION:
Refrigerator Temp (2-6 degrees Celsius)
URINE PROCESSING: (Time frame)
Within 2 hours
URINE PROCESSING:
-Physical Examination (Color and Clarity)
-Chemical Examination (SG, pH, Glucose and Protein)
-Microscopic Examination (WBC, RBC, Bacteria, Epithelial Cells)
SEMEN COLLECTION:
collected on-site
SEMEN CONTAINER:
Sterile
Wide-mouth container
SEMEN TEMPERATURE:
Must not be refrigerated but kept as close to body temperature
SEMEN PROCESSING: ( Time frame)
Within 2 hours
SEMEN PROCESSING:
-Volume
-Sperm count, movement and shape
White Blood Cells
SPUTUM COLLECTION:
Deep respiratory secretions
not saliva
SPUTUM VOLUME:
about 1 teaspoon (5 milliliters)
SPUTUM PROCESSING: (Time frame)
Immediately
SPUTUM PROCESSING:
-Gram Stain
-Sputum Culture
-AFB
STOOL COLLECTION:
Freshly Collected
STOOL VOLUME:
0.5 to 1.0 gram
pea-sized
STOOL PROCESSING: (Time frame)
Within 2 hours
STOOL PROCESSING:
-Physical Examination (Color and Consistency)
-Microscopic Examination (WBCs, RBCs, Parasite, Yeast)
NASOPHARYNGEAL WASH/ASPIRATE
OPTIMAL TIMING:
Within 3 days and not later than 7 days
NASOPHARYNGEAL WASH/ASPIRATE
SPECIMEN COLLECTION:
Insert tubing into the nostril parallel to the palate.
NASOPHARYNGEAL WASH/ASPIRATE
SPECIMEN HANDLING:
Sterile vials @ 4 degrees Celsius
OROPHARYNGEAL (OP) AND
NASOPHARYNGEAL (NP) SWABS
OPTIMAL TIMING-
Within 3 days
No later than 7 days
OROPHARYNGEAL (OP) AND
NASOPHARYNGEAL (NP) SWABS
SWAB TYPE-
Sterile Dacron or rayon swabs with plastic shafts
OROPHARYNGEAL (OP) AND
NASOPHARYNGEAL (NP) SWABS
SPECIMEN HANDLING-
Immediately into a sterile vial with 2 mL of viral transport media w/o antibiotics
OP COLLECTION-
Posterior pharynx and tonsillar areas
NP COLLECTION-
Nares parallel to plate