NON-ACID FAST, AEROBIC GRAM-POSITIVE ACTINOMYCETES

Question 1

• Gram-positive and rod-shaped with branching
  filaments
• Found in soil

Answer 1

STREPTOMYCES

Question 2

BHI agar and SDA

Answer 2

CULTURE MEDIA ON STREPTOMYCES

Question 3

Colonies are dry to chalky and heaped;
some exhibit a grayish white color and “musty
basement odor”

Answer 3

cULTURE ON STREPTOMYCES

Question 4

Susceptible
to lysozyme

Answer 4

Differential test for streptomyces

Question 5

Streptomyces somaliensis
(Agent of Actinomycotic mycetoma)

Answer 5

Human pathogens

Question 6

Microscopic and colony
morphology of the
species of this genus is
very similar to that of
the Nocardia species.

Answer 6

ACTINOMADURA

Question 7

“Molar tooth” appearance

Answer 7

ACTINOMADURA

Question 8

Gram positive actinomycete and facultative, intracellular pathogen

Answer 8

TROPHERYMA WHIPPLEI

Question 9

Etiologic agent of TROPHERYMA WHIPPLEI

Answer 9

: Whipple’s disease

Question 10

• Affects the gastrointestinal tract, joint and muscles and is characterized by abdominal pain and diarrhea.
• Isolated from human feces, saliva and gastric secretions

Answer 10

TROPHERYMA WHIPPLEI

Question 11

(+) Periodic Acid-Schiff Staining (PAS) macrophages

Answer 11

Diagnostic Test of TROPHERYMA WHIPPLEI

Question 12

1. Encephalopathy (inflammation of brain)
2. Lymphadenopathy
3. Malabsorption and diarrhea
4. Arthritis
5. (+) PAS stain (Periodic acid–Schiff)
6. Demonstrating a tropherphyma whippli bacilli within the macrophages

Answer 12

WHIPPLE’S DISEASE