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BACTE FINALS 2.2 > BACILLUS > Flashcards

BACILLUS Flashcards

1
Q

Large, ‘boxcar’ shaped, ± spores

A

Bacillus, Clostridium

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2
Q

Small, pleiomorphic, angular arrangements

A

Corynebacterium
Cutibacterium
Lactobacillus
Listeria

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3
Q

Beading, filamentous

A

Nocardia, Actinomyces

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4
Q

Spore-forming, aerobic or facultatively anaerobic, rod-shaped bacteria and isolated from the soil

A

BACILLUS

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5
Q

BACILLUS SPECIES

A

o Bacillus anthracis
o Bacillus cereus
o Bacillus thuringiensis o Bacillus mycoides

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6
Q

large, boxcar-shaped, gram-positive
rods with clear, unstained, central spores or
“empty spaces”

A

Bacillus on microscopy

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7
Q

Motile with peritrichous flagella except for

A

Bacillus anthracis and Bacillus mycoides

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8
Q

Species only form endospores aerobically
Survive in extreme environment

A

Bacillus

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9
Q

(+) catalase
ferments glucose
hydrolyzes starch

A

Biochemical Test of Bacillus

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10
Q

D-glutamic acid capsule and
protein exotoxins

A

VF of Bacillus

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11
Q

Thiamine B

A

Growth Factor of B anthracis

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12
Q

Gram-positive, large,
encapsulated and square-ended rod; has “bamboo fishing rods” appearance on the unstained central spore

A

Microscopy of Bacillus anthracis

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13
Q

colonies have “Medusa head” appearance with swirling projections; are non- haemolytic; and “beaten egg white” appearance when inoculating loop is used.

A

Bacillus anthracis on BAP

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14
Q

Malignant pustule, sputum and blood

A

Specimen for B. a

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15
Q

isolated from normally sterile site such as blood, lung tissue and CSF.

A

B. anthracis

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16
Q

o Used of old culture in Gram staining:
Gram variable
o Spore stains: Malachite green and
McFadyean stain
o Capsule stain: India ink (blood and
CSF)
o Fluorescent microscopy: rapid
presumptive diagnosis

A

Staining

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17
Q

Used of old culture in Gram stainin

A

Gram variable

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18
Q

Spore stains

A

Malachite green and
McFadyean stain

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19
Q

Capsule stain

A

India ink (blood and
CSF)

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20
Q

rapid
presumptive diagnosis

A

Fluorescent microscopy

21
Q

: BAP, CAP, egg yolk
agar (EYA), Phenylethyl alcohol (PEA),
Polymyxin-lysozyme-EDTA-thallous
acetate (PLET), bicarbonate agar, and
nutrient broth

A

Culture media used in Bacillus anthracis

22
Q

application of heat or alcohol shock
technique before plating on media

A

Enrichment and Selective technique

23
Q

recommended for identification of
B. anthracis in fecal specimen

A

PEA

24
Q

used in isolating
Bacillus from contaminated specimen

A

PEA and PLET

25
Q

determine B. anthracis has
produced lecithinase

A

EYA

26
Q

inverted pine tree
appearance.

A

Gelatine Medium

27
Q

Disease primarily affects: goats and sheep, by
feeding on plants that are contaminated with the
spores.

A

Anthrax

28
Q

inhalation of spores during exposure to
infected animals and contaminated animal
products, or cuts through skin

A

MOA for anthrax

29
Q

: cause signs and symptoms

A

Exotoxins

30
Q

o spores are inoculated into a lesion on
the intestinal mucosa following their
ingestion.

A

Gastrointestinal anthrax

31
Q

o inhalation of spores into the pulmonary
parenchyma
o resembles URTI with mild fever,
dyspnea
o this is usually acquired when getting
sheep’s fur.

A

Pulmonary anthrax/Woolsorter’s disease

32
Q

o Acquired thru skin cuts and abrasions
o Small papule 2-5 days after exposure
o Appearance: “black eschar”, black,
necrotic and painless central area that
does not produce pus.

A

Cutaneous anthrax

33
Q

o Detects thermostable anthrax antigens
o (+): formation of precipitin band after
less than 15 minutes

A

Ascoli Test (Precipitin test)

34
Q

Bacillus (catalase +); Clostridium
(catalase -)

A

Catalase Test

35
Q

Variable (anthrax)

A

Oxidase Test

36
Q

(+) cell wall polysaccharide and capsule
antigen.

A

DFA Test (Direct Fluorescent Antibody Test)

37
Q

Causes food poisoning due to the ingestion of
contaminated cooked rice dishes or other food
products

A

BACILLUS CEREUS (“FRIED RICE” BACILLUS)

38
Q
  • Commonly encountered Bacillus in opportunistic
    infections that causes eye and ear infection.
  • Exhibits motility and resistant to penicillin
A

BACILLUS CEREUS (“FRIED RICE” BACILLUS)

39
Q

uspected food
(>105cells/gram)

A

Best specimen for testing for Bacillus cereus

40
Q

Enterotoxins (heat-stable
and heat-labile), cerelysin, phospholipase C and
pyogenic toxin

A

VF of B. cereus

41
Q

Ferments salicin; (+)
lecithinase

A

Biochemical test of B. cereus

42
Q

large and feathery; spreading growth;
have a frosted-glass appearance and bhaemolytic

A

B. cereus on BAP

43
Q

o Heat labile enterotoxin
o Ingestion of contaminated meat, poultry
and vegetables. IP: 8 to 16 hrs

A

Diarrheal type

44
Q

o Heat stable enterotoxins
o Ingestion of improperly cooked rice

A

Emetic Type

45
Q

o Most commonly encountered laboratory
contaminant
o Halophilic organisms, up to 7% NaCl
o Source of Bacitracin antibiotics
o Cause: Eye infection among prohibited
drug users
o BAP: large, flat, and dull with groundglass appearance; maybe b-haemolytic
and exhibit pigment (pink, yellow,
orange or brown)
o Biochemical test: Ferment mannitol,
xylose and arabinose

A

Bacillus subtilis (Hay Bacillus)

46
Q

o biological indicator: sterilization method
o BAP: large and moist; blister-like
appearance and b-haemolytic

A

Bacillus pumilus

47
Q

o insect pathogens
o produces parasporal crystals that can
be utilized as pesticide

A

Bacillus thuringiensis

48
Q
  • Grow aerobically or anaerobically
  • Not part of normal flora and not highly
    contagious
  • Non-motile, halophilic organism can withstand
    up to 7% NaCl
  • Used as biological weapon of mass destruction
  • Grows in low pH (<6.0) environment, produce
    lecithinase and ferments glucose
A

Bacillus anthracis

BACTE FINALS 2.2 (8 decks)

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