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BACTE FINALS 2.2 > CORYNEBACTERIUM > Flashcards

CORYNEBACTERIUM Flashcards

1
Q
  • Catalase positive, gram-positive rods
  • Non–acid-fast, non–spore-forming, and mostly
    non-branching rods
A

CORYNEBACTERIUM

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2
Q

C. jeikeiumand C. urealyticum

A

Lipophilic Corynebacteria

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3
Q

o C. diphtheriae, C. pseudotuberculosis,
C. pseudodiphtheriticum, C. striatum, C.
ulceransand C. xerosis

A

Non-Lipophilic Corynebacteria

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4
Q

a can be divided into non lipophilic
and lipophilic species
* Majority of the species are found as indigenous
microbiota of the skin and mucous membrane of
humans and animals. Species are pathogenic to
animals, plants, and humans.

A
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5
Q

found in fresh water.
Corynebacterium are also found in salt water,
soil, and air

A
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6
Q

Species are non-motile, non-encapsulated, nonspore forming and highly theomorphic rod

A
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7
Q

Specie are glucose and maltose fermenters
except

A

C. pseudodiphthericum and C. urealyticum.

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8
Q

slightly curved, grampositive rods

A
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9
Q

“club shape” or coryneform

A
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10
Q

Cells are arranged singly, in “palisades” of
parallel cells or in pairs of cells connected after
cell division to form V or L shapes: “Chinese
letters” appearance

A
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11
Q

“diphtheria-like

A
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12
Q

Specie causing human infections in Corynebacterium

A

C.
diphtheriae, C. jeikeium, C. pseudotuberculosis,
C. pseudodiphthericum, C. ulcerans, and C.
urealyticum

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13
Q

Majority of the species are found as indigenous microbiota of the skin and mucous membrane of humans and animals. Species are pathogenic to animals, plants, and humans.

A

CORYNEBACTERIUM

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14
Q

AKA: diphtheria bacillus or Kleb-Loffler bacillus

A

CORYNEBACTERIUM DIPHTHERIAE

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15
Q

Facultative anaerobe, Inhabits the human nasopharynx in carrier

A

CORYNEBACTERIUM DIPHTHERIAE

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16
Q

inhalation of contaminated respiratory droplets or direct contact with infected cutaneous lesions.

A

MOA for Corynebacterium diphtheriae

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17
Q

Readily killed by heat and by most of the usual disinfectants.
• Glucose and maltose fermenter

A

CORYNEBACTERIUM DIPHTHERIAE

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18
Q

VF OF CORYNEBACTERIUM DIPHTHERIAE

A

Diphtheria toxin

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19
Q

Preferred medium for Preferred medium

A

Enriched medium with
serum, cysteine and potassium tellurite

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20
Q

o Rounded ends and “club-shaped swelling”
o highly pleomorphic cells are arranged in pairs and create X, V, Y and L formation that closely resembles Chinese letters.

A

Microscopy of CORYNEBACTERIUM DIPHTHERIAE

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21
Q

(-) Urease; (+) Nitrate reduction.

A

Biochemical Test for CORYNEBACTERIUM DIPHTHERIAE

22
Q

Nasopharyngeal and throat swabs

A

Best specimen for CORYNEBACTERIUM DIPHTHERIAE

23
Q

THREE BIOTYPES OF CORYNEBACTERIUM DIPHTHERIAE

A

• Intermedius
• Mitis
• Gravis

24
Q

Very small, flat, dry, and grayish-black
colonies; non-haemolytic

A

Intermedius

25
Q

Small, black and convex that have
“fried-egg appearance

A

Mitis

26
Q

Large, flat and dark gray colonies
“daisy-head appearance”

A

Gravis

27
Q

used for the visualization of metachromatic granules which is also known as Much granules.

A

Albert stain

28
Q

• Heat-labile
• Produced by strains with a lysogenic b-phage
that carries the TOX gene.

A

DIPHTHERIA TOXIN

29
Q

• Tissue necrosis and exudates formation
(psedomembrane lining) over the tonsils, larynx
and pharynx
• Alkaline pH (7.8 to 8.0), aerobic environment
and sufficient amount of iron in the medium.

A

DIPHTHERIA TOXIN

30
Q

Acute, infectious disease characterized by production of systemic toxin and false membrane lining (Pseudomembranous formation) of the throat mucous membrane → respiratory obstruction.

A

RESPIRATORY DIPHTHERIA

31
Q

(+): 4 mm to 5 mm fine precipitin lines at 45 angle to the streaks.

A

Immunodiffusion Test - Elek’s Test

32
Q

AKA: diphtheria antitoxin test (in
vivo test)

A

Guinea pig lethal Test

33
Q

detection of diphtheria antitoxin
(in vitro)

A

Tissue Culture Test

34
Q

used for detection of toxin
genes

A

Polymerase Chain Reaction

35
Q

Preferred medium
for isolation and identification of
crynebacterium

A

Cystine Tellurite Blood Agar

36
Q

o Modification of
the Tinsdale agar o Selective and
Differential
o Contains sheep blood, bovine serum,
cysteine

A

Cystine Tellurite Blood Agar

37
Q

Positive reaction for Cystine Tellirite Blood Agar

A

Colonies of corynebacterial exhibit a
black or brown color after 48 hours

38
Q

C. diphtheriae, C. ulcerans and C.
pseudotuberculosis.

A

Positive organism for CTBA

39
Q

Sheep blood,
cystine, potassium tellurite and sodium thiosulfate

A

Tinsdale Agar

40
Q

o (+) Black color surrounded by halo
o (+) all bio types of C. diphtheriae

A

Tinsdale Agar

41
Q

Urea production: C. urealyticum

A

Christensen Urea Slant

42
Q

Microscopic and metachromatic
granules of C.
diphtheriae.

A

Pai’s Slant or Loeffler’s Serum Agar

43
Q

(+): C. diphtheriae
exhibit “poached- egg” appearance

A

Pai’s Slant or Loeffler’s Serum Agar

44
Q

Metachromatic diphtheriaeare called

A

Babes ernst bodies

45
Q

o Used to determine the susceptibility of a
person to diphtheria.
o Involves the intradermal introduction of
a small amount of the diphtheria toxin
into the arm of the suspected individual.

A

Schick Test

46
Q

(+): Redness and Swelling around the
site.

A

Schick Test

47
Q

Most frequently isolated and most clinically significant.
• Urinary pathogen, strict aerobe and lipophilic
• Do not ferment glucose and maltose
• Microscopy: V shaped and palisades
• Culture: BAP –pinpoint, white smooth, non-
haemolytic
• Urease producer
• Most frequent isolated and most clinically
significant among them all.

A

C. UREALYTICUM

48
Q

• Normal flora of nasopharynx
• Respiratory infection, UTI, cutaneous wound
infection in immunocompromised.
• Microscopy: arranged in parallel rows or
palisades and do not exhibit any other characteristics “pleomorphism” that is similar to other.
• Urease and nitrate positive

A

C. PSEUDODIPHTHERITICUM

49
Q

• Skin normal flora in inguinal, axillary and rectal sites.
• Obligate aerobe and antibiotic resistant
• Immunocompromised, Common cause of diphtheroid prosthetic valve endocarditis in
adults.
• Microscopy: Pleomorphic, club-shaped and
arranged in V-shaped
• Urease and Nitrate negative

A

C. JEIKEIUM

50
Q

• Animal contact and unpasteurized dairy products.
• Skin ulcers and exudative pharyngitis
• Associated with diphtheria-like sore throats.
• Culture: BAP-narrow zone of b-hemolysis
• CTBA - brown halo
• Loeffler’s serum agar: Exhibit growth
• (+): Urease and gelatinase; (-) nitrate reduction.

A

C. ULCERANS

51
Q

• Animal pathogens that human can contract thru direct contact with infected animals.
• Dermonecrotic toxin causes death of various cell types
• Diphtheria toxin (causative agent)
• Culture: CTBA –black color and surrounded by
brown halo
• BAP: small and yellowish-white
• (+) urease; (-) gelatinase

A

C. PSEUDOTUBERCULOSIS

52
Q

Preferred for collection.

A

Calcium alginate swa

BACTE FINALS 2.2 (8 decks)

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