ARCANOBACTERIUM, KURTHIA, GARDENLLA VAGINALIS, AEROBIC ACTINOMYCES Flashcards
Classified as pleomorphic, non-motile, rod-
shaped and Gram-positive
ARCANOBACTERIUM
colonies have a narrow zone of b-
hemolysis; exhibit pitting of the agar with a black
opaque dot.
ARCANOBACTERIUM on BAP
lipase and lecithinase
positive and Positive reverse CAMP reaction due to Phospholipase D.
A. haemolyticum
Obligate aerobe, gram positive rods, Motile by peritrichous flagella
KURTHIA
Exhibit a Rhizoid growth
Nutrient agar on Kurthia
Large
“medusa-head” appearance
BAP on Kurthia
Short, pleomorphic gram-positive rod or coccobacillus that often stains gram-variable or gram-negative.
GARDNERELLA VAGINALIS
(causative agent) → bacterial vaginosis (BV) in humans
GARDNERELLA VAGINALIS
The observation of “clue cells,” large squamous epithelial cells with gram-positive and gram- variable bacilli and coccobacilli clustered on the edges, aids the diagnosis of BV
GARDNERELLA VAGINALIS
Vaginal discharge
collected from suspected BV
Best specimen for G vaginalis
grows best in 5% to 7%
CO2 at a temperature of 35°C to 37C.
G. vaginalis
human blood bilayer Tween (HBT)
agar
medium of choice for G. vaginalis
contains human
blood and is used for recovery of this
organism.
V (vaginalis) agar
Gram positive bacilli, Aerobes with a branching filamentous growth that extends along the agar due to the substrate hyphae and into the agar due to the aerial hyphae.
• Filamentous rods with beaded appearance
AEROBIC ACTINOMYCETES
Found in the soil and organic materials
• Can cause disease to humans and many
animals
AEROBIC ACTINOMYCETES