Next generation sequencing

Question 1

NGS is good at

Answer 1

generating BIG DATA

Question 2

used for

Answer 2

• determining the DNA sequence of large numbers of different DNA molecules simultaneously
• genome sequencing
• cDNA sequencing

Question 3

process of NGS

Answer 3

1) take genome and fragment
2) machines then separate each fragment into their own area of the machine
3) these fragments are amplified using PCR involving fluorescent labelled nucleotides
4) each nucleotide is labelled with a different fluorescent dye
5) the different coloured nucleotides ae added sequentially
6) until order of the nucleotides is sequenced

Question 4

computer is used to

Answer 4

rebuild fragments looking for overlapping sequences to assemble the genome

Question 5

how are fragments rejoined by the computer

Answer 5

looking for overlapping sequences to assemble the genome

Question 6

applications of NGGS

Answer 6

-sequences individual genomes

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Question 7

NGS can even look for

Answer 7

point mutations

Question 8

why is there a need for deep sequencing

Answer 8

multiple reading are need to reduce error so we can be sure sequences we are looking at are true reflections

Question 9

quite often only …. are sequenced

Answer 9

NGS

Question 10

many different

Answer 10

NGS methods