Neutrophil Arrival/Functions

Question 1

What kind of inflammation is associated with neutrophils?

Answer 1

Acute inflammation

Question 2

What is margination?

Answer 2

Blood flow stasis-> leukocytes pushed to periphery

Question 3

What is Sialyl-Lewis X?

Answer 3

Expressed on leukocytes

Binds to endothelial cell selectins during rolling

Question 4

What up regulates CAM?

What does it do?

Answer 4

TNF, IL-1

CAM binds to integrins on neutrophils-> adhesion

Question 5

What up regulates integrins?

What do they do?

Answer 5

C5a, LTB4

Bind to CAM on endothelium-> neutrophil adhesion

Question 6

After neutrophils transmigrate, what initiates chemotaxis?

Answer 6

IL-8, C5a, LTB4

Question 7

What induces neutrophil opsonization/phagocytosis?

Answer 7

C3b, IgG

Question 8

What is opsonization?

Answer 8

Pathogen is “marked” for phagocytosis/ingestion by opsonins such as IgG and C3b

Question 9

What are the characteristics of chronic granulomatous disease?

Answer 9

Defect in neutrophils- unable to properly use O2 dependent killing.
Instead, body “walls-off” pathogens with granulomas

Question 10

What does myeloperoxidase do?

Answer 10

Converts H2O2 to Hypochlorate (HOCL) in neutrophils. HOCL used for pathogen killing

Question 11

What happens in Chediak-Higashi syndrome?

Answer 11

Microtubules fail to polymerize
Lysosome and Phagosome cannot fuse
Impaired pathogen killing