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histology exam > Nervous Tissue > Flashcards

Nervous Tissue Flashcards

1
Q

Which dyes are best for demonstration of Nissl substance?

A
  • Cresyl echt
  • Thionin
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2
Q

What is the purpose of using Weigert’s?

A

Demonstration of myelin

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3
Q

What will myelin look like after staining with Weigert’s?

A

Blue

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4
Q

What is the purpose of using luxol fast blue?

A

Differentiate white and grey matter

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5
Q

What is the principle of luxol fast blue?

A

Copper phthalocyanine dye which is soluble in organic solvent.

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6
Q

What are idea fixatives for luxol fast blue

A
  • NBF
  • K dichromate
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7
Q

What size of sections are required for demonstrations of myelin sheath?

A

10-15 microns

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8
Q

What will grey matter look like after staining with luxol fast blue?

A

colourless

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9
Q

What will white matter look like after staining with luxol fast blue?

A

Blue

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10
Q

What will demyelinated myelin look like after staining with luxol fast blue?

A

colourless

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11
Q

What will Nissl substance look like after staining with luxol fast blue?

A

purple violet

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12
Q

What is the purpose of the Weil’s method?

A

Demonstrate myelin

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13
Q

What is the first demonstration step in Weil’s method?

A

Ferric ammonium sulfate

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14
Q

What is the second demonstration step in the Weil’s method?

A

Borax ferricyanide

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15
Q

What is the best fixative to use for Weil’s method?

A

NBF

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16
Q

What will myelin look like after being stained with Weil’s method?

A

Blue / Black

17
Q

What will the background look like after being stained with Weil’s method?

A

light Tan

18
Q

What is the purpose of Mallory’s phosphotungstic acid?

A

Demonstrate glial cells

19
Q

What is the idea fixative for Mallory’s phosphotungstic acid?

A

NBF

20
Q

What size of sections should be used for the demonstration of glial cells?

A

6 - 8 microns

21
Q

What will glial cells look like after being stained with mallory’s?

A

Blue

22
Q

What will nuclei look like after being stained with mallory’s?

A

Blue

23
Q

What will myelin look like after being stained with mallory’s?

A

Blue

24
Q

What will neurons look like after being stained with mallory’s?

A

salmon

25
Q

What is the purpose of the cajal’s method?

A

Demonstrate astrocytes

26
Q

What is the idea fixative for cajal’s method?

A
  • Formalin ammonium bromide
27
Q

What will astrocytes look like following staining with cajal’s mehtod?

A

Black

histology exam (8 decks)

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