Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Histology Exam > Microorganisms > Flashcards

Microorganisms Flashcards

1
Q

What is the purpose of gram staining tissue sections?

A

Differentiate/ demonstrate gram positive and negative organisms

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What is the principle of gram staining?

A

Physical theory - porosity

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What is the difference between a mordant and a trapping agent?

A

Mordant:
- Used with acidic dyes, usually metallic and is applied before the dye.
Trapping agent:
- used with basic dyes, are not usually metallic and is applied after the dye but prior to removing excess dye.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What are modifications of the gram stain? what is the purpose of modifying it?

A
  • Gram twort
  • Brown and Brenn
  • Addition of a 3rd dye to enhance gram negative organsim
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What will Gram Twort stain?

A

Cytoplasm = green

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What will Brown and Brenn stain?

A

Cytoplasm = yellow

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What is the purpose of the Ziehl-Neelsen method?

A

Demonstration of acid fast bacteria in tissue?

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What is the principle behind acid fast stains?

A

Chemical vs solubility

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What is the counterstain in the Ziehl-Neelsen method?

A

Methylene Blue

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What is the primary stain in the Ziehl-Neelsen method? And what does it target?

A

Basic Fuchsin - Mycolic acid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What will acid fast bacili stain after using the Ziehl-Neelsen method?

A

Red

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

How should sections be decolourized in the Ziehl-Neelsen method?

A

1% HCl + 70% ethanol

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What are the modifications of the Ziehl-Neelsen method?

A
  • Kinyoun - cold
  • Fite - enhancement
  • Auramine and Rhodamine fluorescence
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What is the purpose of using Fite in the demonstration of acid fast bacteria?

A

enhance weakly acid fast bacteria

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What substances are used for the fite method for demonstrating acid fast bacteria?

A

Peanut oil & xylene

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What is the purpose of the warthin starry method?

A

Demonstration of spirochetes

17
Q

What is the principle behind the Warthin starry method?

A

Argyrophilic reactions

18
Q

What is the reducing agent used in the Warthin starry method?

A

hydroquinone

19
Q

What will spirochetes, H.pylori and klebsiella stain following the Warthin starry method?

A

Black

20
Q

What will nuclei stain following the Warthin starry method?

A

Brown

21
Q

What will the background stain following the Warthin starry method?

A

golden Yellow

22
Q

You overstain a sections containing spirochetes with the Warthin starry method, how would you proceed?

A
  • Remove the stain with iodine and hypo
  • Re-stain
23
Q

What is the purpose of the Steiner and Steiner method?

A

Demonstrate spirochetes, H.pylori, cat scratch, legionella and pneumophilia

24
Q

What is the principle of the Steiner and Steiner method?

A

Argyrophilic reactions

25
Q

What is the reducing agent in the Steiner and Steiner method?

A

Gum mastic + hydroquinone + absolute alcohol

26
Q

What will the bacteria look like following staining with the Steiner and Steiner method?

A

Brown / black

27
Q

What will the background look like following staining with the Steiner and Steiner method?

A

Light yellow

28
Q

What are the 5 method to H.pylori identification.

A
  • Diff quick
  • Dye-based histochemical stains
  • True Giemsa stain
  • Silver stains
  • IHC
29
Q

What are 4 demonstration methods of fungus?

A
  • H&E
  • Gram stains
  • PAS
  • Silver impregnation
30
Q

What is the purpose of Grocott’s Methenamine silver?

A

Demonstrate fungi, actinomyces and norcardia.

31
Q

What is the principle of Grocott’s Methenamine silver?

A

Argentaffin reactions

32
Q

Which fixative should be avoid when staining with Grocott’s Methenamine silver? WHY?

A

Glutaraldehyde - because it contains free aldehydes which will reduce silver.

33
Q

what is the counterstain in Grocott’s Methenamine silver method?

A

Light green

34
Q

What will fungi stain following Grocott’s Methenamine silver method?

A

Black

35
Q

What should you do prior to staining a liver section with Grocott’s Methenamine silver method?

A

Diastase to remove glycogen

Histology Exam (8 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions