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Histology Exam > Lipids > Flashcards

Lipids Flashcards

1
Q

What are the histological characteristics of lipids?

A
  • soluble in organic solvents
  • sudanophilic
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2
Q

What are simple lipids?

A
  • fatty acids
  • triglycerides
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3
Q

What are examples of compound lipids?

A
  • phospholipids
  • glycolipids
  • sulfolipids
    (Fatty acids + Alc + Phos/Nitro)
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4
Q

what are derived lipids?

A

simple or compound lipid formed through hydrolysis
(cholesterol, bile acids, hormones)

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5
Q

What is the fixative of choice for lipid demonstration?

A

Osmium tetroxide and chromic acid

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6
Q

what is the best demonstration method for lipids?

A

Fresh Frozen

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7
Q

What dyes are idea for the demonstration of lipids?

A

Lipofuchsins

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8
Q

What principle must be used to demonstrate lipids?

A

Physical method

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9
Q

What form of chromogens do lipid dyes form?

A

Lysochromes

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10
Q

What is herxheimer’s fluid ?

A

Equal parts acetone and 70% alcohol

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11
Q

What is the purpose of using Sudan black B?

A

demonstrate neutral lipids, triglycerides, phospholipids

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12
Q

What is the idea fixative to use for sudan black b techniques?

A
  • NBF
  • Calcium formalin
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13
Q

How big should sections be for lipid demonstration?

A

10 microns

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14
Q

What will lipids look like after being stained with sudan black b?

A

Blue / Black

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15
Q

What will nuclei look like after being stained with sudan black b?

A

red

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16
Q

What is the purpose of staining with Oil red O?

A

Distinguish fine lipid droplets vs glycogen and neutral lipids

17
Q

what is the principle of Oil red O?

A

selective solubility

18
Q

What is the fixative of choice for Oil red O technique?

A
  • NBF
  • Calcium formol
19
Q

What will fat globules look like after staining with oil red o?

A

intense red

20
Q

what will nuclei look like after staining with oil red o?

A

blue

21
Q

What is the purpose of staining using osmium tetroxide?

A

Lipid preparation for electron microscopy

22
Q

what is the only fixative that can be used to completely fix lipids?

A

osmium tetroxide

23
Q

what will lipids look like after being stained with osmium tetroxide?

A

Black

24
Q
A

Histology Exam (8 decks)

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