Lipids Flashcards
What are the histological characteristics of lipids?
- soluble in organic solvents
- sudanophilic
What are simple lipids?
- fatty acids
- triglycerides
What are examples of compound lipids?
- phospholipids
- glycolipids
- sulfolipids
(Fatty acids + Alc + Phos/Nitro)
what are derived lipids?
simple or compound lipid formed through hydrolysis
(cholesterol, bile acids, hormones)
What is the fixative of choice for lipid demonstration?
Osmium tetroxide and chromic acid
what is the best demonstration method for lipids?
Fresh Frozen
What dyes are idea for the demonstration of lipids?
Lipofuchsins
What principle must be used to demonstrate lipids?
Physical method
What form of chromogens do lipid dyes form?
Lysochromes
What is herxheimer’s fluid ?
Equal parts acetone and 70% alcohol
What is the purpose of using Sudan black B?
demonstrate neutral lipids, triglycerides, phospholipids
What is the idea fixative to use for sudan black b techniques?
- NBF
- Calcium formalin
How big should sections be for lipid demonstration?
10 microns
What will lipids look like after being stained with sudan black b?
Blue / Black
What will nuclei look like after being stained with sudan black b?
red
What is the purpose of staining with Oil red O?
Distinguish fine lipid droplets vs glycogen and neutral lipids
what is the principle of Oil red O?
selective solubility
What is the fixative of choice for Oil red O technique?
- NBF
- Calcium formol
What will fat globules look like after staining with oil red o?
intense red
what will nuclei look like after staining with oil red o?
blue
What is the purpose of staining using osmium tetroxide?
Lipid preparation for electron microscopy
what is the only fixative that can be used to completely fix lipids?
osmium tetroxide
what will lipids look like after being stained with osmium tetroxide?
Black
