Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Histology Exam > Carbohydrates > Flashcards

Carbohydrates Flashcards

1
Q

Which carbohydrates cannot be demonstrated in tissue sections?

A
  • Glucose
  • Oligosaccharides
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What are the 4 groups of naturally occuring polysaccharides?

A

Group 1: Neutral polysaccharides
Group 2: Acid mucopolysaccharides
Group 3: Glycoproteins
Group 4: Glycolipids

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What is the purpose of PAS?

A

Demonstrate glycogen, neutral mucopolysaccharides, basement membrane, and fungal infections

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What is the principle of PAS?

A

Glycol groups are oxidized to aldehydes which react with the Schiff reagent to produce a colour

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What will PAS positive structures stain?

A

Magenta

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What will nuclei look like after staining with PAS?

A

Blue

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What are the storing conditions of Schiffs reagent?

A
  • sealed in dark cool (4C) place
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What is the BEST fixative to use for PAS?

A

Alcohol or alcoholic picric acid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What is dPAS?

A

Diastase method, enzymatic treatment of sections with diastase to digest glycogen, followed by PAS.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What is the result of dPAS method?

A

Undigested sections - PAS positive magenta staining
Digested sections - No magenta

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What is the purpose of Alcian blue at a pH of 2.5?

A

Demonstrate carboxylated and sulphated acid mucopolysaccharide

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What is the purpose of Alcian Blue at a pH of 1.0?

A

Demonstration of sulphated mucins.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What is the ideal fixative to use for the Alcian blue methods?

A
  • 10% NBF
  • Bouins
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What is the purpose of staining with both Alcian blue and PAS?

A

To distinguish neutral and acid mucosubstances

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What is the purpose of treating slides with hyaluronidase prior to staining with Alcian Blue?

A

Remove hyaluronic acids which inhibits or reduces staining quality.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What is the purpose of staining with mucicarmine?

A

Demonstrate epithelium mucin or encapsulated fungi .

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

What does mucicarmine stain? Be specific!

A

carboxylated and sulphated mucins

18
Q

What will mucins look like after staining with mucicarmine?

A

deep red

19
Q

What is the counterstain used in mucicarmine?

A

metanil yellow

20
Q

What is the purpose of using colloidal iron?

A

To demonstrate;
- acid mucins
- cryptococcus neoformans
- carboxylated and sulfated mucopolysaccharides
- glycoproteins

21
Q

What is the principle of colloidal iron?

A

Histochemical reaction

22
Q

Which fixatives are idea for colloidal iron?

A
  • NBF
  • Carnoy’s
23
Q

what is the purpose of congo red?

A

demonstrate amyloid deposits in tissue sections.

24
Q

Which fixatives should be avoided with colloidal iron?

A

Chromate fixatives

25
Q

What will acid mucopolysaccharides look like after using colloidal iron?

A

deep blue

26
Q

What is the counterstain used in colloidal iron?

A

pink / red

27
Q

What is the principle which congo red uses?

A

Chemical theory

28
Q

What is the ideal fixative to use when staining with congo red?

A
  • Alcohol
  • NBF
29
Q

What will amyloid look like when stained with congo red?

A

Pink

30
Q

What will nuclei look like when stained with congo red?

A

Blue

31
Q

What is the purpose of staining with crystal violet in carbohydrate demonstrations?

A

Rapid screening to demonstrate amyloid

32
Q

What is the principle in which crystal violet works?

A

Monochromatic staining

33
Q

What is the idea fixative to use when staining with crystal violet?

A

NBF

34
Q

What section width should be used for crystal violet methods?

A

10 - 12 microns

35
Q

If you want to preserve a slide stained with crystal violet, how would you do it?

A
  • Aqueous mounting media
  • Seal the edges
36
Q

What will amyloid look like after staining with crystal violet?

A

Purple

37
Q

What is the purpose of staining with Thioflavine?

A

Demonstrate amyloid

38
Q

What is the principle in which thioflavine works?

A

Fluorescence

39
Q

In order to see the amyloid fluoresce using thioflavine what must you do first?

A

Quench the background with alum hematoxylin

40
Q

what is the ideal fixative to use with thioflavine method?

A

NBF

41
Q

What size of sections should be used with the thioflavine technique?

A

6 - 10 microns

42
Q

What will amyloid look like after stained with thioflavine?

A

fluorescent yellow / yellow- green

Histology Exam (8 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions