Nerve Staining

Question 1

Define: Neuron

Answer 1

A nerve cell

Question 2

Define: Glia and glial fibers

Answer 2

Cells that provide the supporting network for the central nervous system. 4 types - oligodendroglia, astroglia, microglia, and ependymal

Question 3

Define: Nissl substance

Answer 3

Clumps of rough, or granular, endoplasmic reticulum. Because of the RNA content, Nissl substance stains sharply with basic aniline dyes, such as thionin and cresyl echt violet

Question 4

Define: Myelin

Answer 4

White fatty material encasing and providing a protective sheath for some nerve fibers

Question 5

Define: Axon (axis cylinder)

Answer 5

The nerve process that conducts impulses away from the cell body of the neuron. Neurons have a single axon. Axons are included along with dendrites in the term “nerve fibers”; axons have also been referred to as the axis cylinder

Question 6

What substance is demonstrated by Cresyl echt violet stain?

Question 7

What substance is demonstrated by Bodian stain?

Question 8

What substance is demonstrated by Holmes silver nitrate stain?

Question 9

What substance is demonstrated by Bielschowsky stain?

Question 10

What substance is demonstrated by Weil stain?

Question 11

What substance is demonstrated by Luxol fast blue stain?

Question 12

What fixative should be used with Cresyl echt violet stain?

Question 13

What fixative should be used with Bodian stain?

Question 14

What fixative should be used with Holmes silver nitrate stain?

Question 15

What fixative should be used with Bielschowsky stain?

Question 16

What fixative should be used with Weil stain?

Question 17

What fixative should be used with Luxol fast blue stain?

Question 18

What are the primary reagents, and their purpose, used in Cresyl echt violet stain?

Question 19

What are the primary reagents, and their purpose, used in Bodian stain?

Question 20

What are the primary reagents, and their purpose, used in Holmes silver nitrate stain?

Question 21

What are the primary reagents, and their purpose, used in Bielschowsky stain?

Question 22

What are the primary reagents, and their purpose, used in Weil stain?

Question 23

What are the primary reagents, and their purpose, used in Luxol fast blue stain?

Question 24

Describe the results seen from the Cresyl echt violet stain?

Question 25

Describe the results seen from the Bodian stain?

Question 26

Describe the results seen from the Holmes silver nitrate stain?

Question 27

Describe the results seen from the Bielschowsky stain?

Question 28

Describe the results seen from the Weil stain?

Question 29

Describe the results seen from the Luxol fast blue stain?

Question 30

What is the appropriate control material for Cresyl echt violet stain?

Question 31

What is the appropriate control material for Bodian stain?

Question 32

What is the appropriate control material for Holmes silver nitrate stain?

Question 33

What is the appropriate control material for Bielschowsky stain?

Question 34

What is the appropriate control material for Weil stain?

Question 35

What is the appropriate control material for Luxol fast blue stain?

Question 36

What are common sources of error for Cresyl echt violet stain and how are they corrected?

Question 37

What are common sources of error for Bodian stain and how are they corrected?

Question 38

What are common sources of error for Holmes silver nitrate stain and how are they corrected?

Question 39

What are common sources of error for Bielschowsky stain and how are they corrected?

Question 40

What are common sources of error for Weil stain and how are they corrected?

Question 41

What are common sources of error for Luxol fast blue stain and how are they corrected?

Question 42

Luxol fast blue