Cytopreparatory Techniques

Question 1

Define: Diagnostic cytology

Question 2

Define: Liquid-based cytology

Question 3

Define: Cross-contamination

Question 4

Define: Cell block

Question 5

Differentiate between gynecologic & nongynecologic specimen.

Question 6

Explain the use and composition of prefixatives.

Question 7

Explain the effect of prefixatives on cellular morphology.

Question 8

Explain the effect of prefixatives on cellular morphology.

Question 9

List acceptable fixatives for cytology.

Question 10

Summarize 3 slide smear methods.

Question 11

Describe methods to handle sparsely cellular specimens.

Question 12

Describe methods to reduce red blood cells in smears.

Question 13

Describe methods increase cellular adherence to slides.

Question 14

7. Describe methods to remove excess mucus.

Question 15

Describe the effect on staining if Carbowax fixatives are not removed before staining.

Question 16

Describe methods for making cell blocks.

Question 17

State the purpose of the Papanicolaou (Pap) stain.

Question 18

State 2 benefits of liquid-based cytology preparation.

Question 19

Outline the Pap staining technique.

Answer 19

Primary reagents and/or dye:
Results of staining:
Potential sources of error and appropriate corrective action:
Mode of action:

Question 20

Discuss how cross-contamination occurs and recommend techniques that can be used to prevent it.