Diagnostic Molecular Techniques Flashcards
List the preanalytical variables associated with various tissue types for molecular diagnostic testing.
Discuss basic molecular biology terms associated with in situ hybridization.
Describe the functional steps of the in situ hybridization staining process/
Compare fluorescent vs chromogenic in situ hybridization.
Describe common troubleshooting in in situ hybridization.
Describe the fundamentals of polymerase chain reaction (PCR).
What is required to prepare a laboratory for molecular testing.
Describe quantitative real-time PCR.
Describe reverse transcriptase PCR (RT-PCR).
Describe Sanger sequencing.
Describe the fundamentals of next-generation sequencing (NGS).
Describe circulating tumor DNA.