Diagnostic Molecular Techniques Flashcards

1
Q

List the preanalytical variables associated with various tissue types for molecular diagnostic testing.

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2
Q

Discuss basic molecular biology terms associated with in situ hybridization.

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3
Q

Describe the functional steps of the in situ hybridization staining process/

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4
Q

Compare fluorescent vs chromogenic in situ hybridization.

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5
Q

Describe common troubleshooting in in situ hybridization.

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6
Q

Describe the fundamentals of polymerase chain reaction (PCR).

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7
Q

What is required to prepare a laboratory for molecular testing.

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8
Q

Describe quantitative real-time PCR.

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9
Q

Describe reverse transcriptase PCR (RT-PCR).

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10
Q

Describe Sanger sequencing.

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11
Q

Describe the fundamentals of next-generation sequencing (NGS).

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12
Q

Describe circulating tumor DNA.

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