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Microbiology CT 4 > Myobacterium > Flashcards

Myobacterium Flashcards

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1
Q

Name selective staining method for Myobacterium

A

Ziehl-Neelsen

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2
Q

What kind of organism is Myobacterium?

A

Saprophyte

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3
Q

What is a saprophyte?

A

Saprophyte is an organism that feeds on dead organic matter

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4
Q

Patogenicity of Myobacterium

A

Majority is apathogenic (do not cause disease). Some are pathogenic and cause chronical diseases in humans and animals (tuberculosis, pneumonia, lepropsy etc.)

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5
Q

Microscopical examination of Myobacterium

A

Gram +
Thin rods
Various length
Non-specific arrangement
Non-motile
Acid-alcohol resistant

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6
Q

Relation to oxygen for Myobacterium

A

aerobic

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7
Q

The cell wall in Myobacterium is rich in

A

lipids and mycolic acids, makes it resistant to the environment

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8
Q

Important species of Myobacteriaceae (5)

A

Myobacterium bovis
Myobacterium avium
Myobacterium tuberculosis
Myobacterium avium subsp. paratuberculosis
Myobacterium leprae

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9
Q

What causes bovine TBC?

A

Myobacterium bovis

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10
Q

What causes avian TBC?

A

Myobacterium avium

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11
Q

What causes human TBC?

A

Myobacterium tuberculosis

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12
Q

What causes para-TBC/ John’s disease in ruminants?

A

Myobacterium avium subsp. paratuberculosis

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13
Q

What causes Crohn’s disease in human?

A

Myobacterium avium subsp. paratuberculosis

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14
Q

What causes lepropsy in humans?

A

Myobacterium leprae

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15
Q

Special test for Myobacterium used for alergenodiagnosis

A

Tuberculination - tuberculin (PPD - purified protein derivate) is administered in 0.1 ml.
Used for alergenodiagnosis - late hypersensitivity reaction.

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16
Q

Cultivation properties of Myobacterium (O2, temp, time)

A

Obligate aerobes
37 degrees Celsius (20-34C), M. avium - 42 degrees C
Generation time: 2-24 hours

17
Q

Incubation times for Myobacteria:

A

Rapid growing - 2-7 days
Slow growing - 7-60 days (for pathogenic species)
Barely culturable - few weeks or months

18
Q

Non-culturable Myobacterium

A

Myobacterium leprae

19
Q

Form of growth of Eugonic Myobacteria + 2 examples

A

Grow upwards (M. tuberculosis and M. avium)

20
Q

Form of growth of Dysgonic Myobacteria

A

Small
Smooth
Shiny
Flat
(M. bovis)

21
Q

Myobacterium can grow in two forms

A

Eugonic or Dysgonic

22
Q

Special nutrient media for Myobacterium spp

A

Liquid Sula medium
Solid egg-based medium: Löwenstein-Jensen agar, Stonebrink agar or Petragini agar

Microbiology CT 4 (6 decks)

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