Brucellaceae Flashcards
Name selective staining method for Brucella
Stamp (positive = red, negative = green)
What type of pathogen are Brucella?
Obligate pathogens - cause zoonoses
Microscopical examination of Brucellaceae
Gram -
Small rods or coccobacilli
No specific arrangement
Non-motile
Acid-resistant
Brucella relation to oxygen
aerobic or microaerophilic
Brucella reaction on catalase, oxidase and urease
Catalase +, oxidase + and urease +
Brucella growth on common nutrient media
Weak
What kind of parasites are Brucellaea?
Intracellular parasites, able to multiply within the macrophages
Brucellaceae affinity to reproductive tract
Cause placentitis, abortus, infertility, orchitis and epididymitis
Virulence factors of Brucellaceae
Endotoxin
Somatic O-antigen
Intracellular parasitism
Important zoonotic species of Brucellaceae (5)
Brucella abortus
Brucella melitensis
Brucella suis
Brucella canis
Brucella neotomae
Bovine brucellosis is caused by:
Brucella abortus
Ovine and caprine brucellosis is caused by
Brucella melitensis
Brucellosis of swine and hares is caused by
Brucella suis
Canine brucellosis is caused by
Brucella canis
Brucellosis in rodents is caused by
Brucella neotomae
Which Brucella is NOT a zoonotic pathogen?
Brucella Ovis
Brucella Ovis causes
infectious epididymitis of rams
How is Brucella excreted?
In bovine milk
Material for cultivation of Brucellaceae
fetus, lavage from uterus, vaginal swabs, lymph nodes, blood or milk
Cultivation properties of Brucellaceae
S (or R) form
Aerobic, microaerophilic (5-10% CO2)
37 degrees Celsius (20-40C)
Incubation time is 3-5 days
Cultivation media for Brucella
enriched media such as blood serum, tissue extract, vitamins or 1% glycerin
Brucella on blood agar
Small
Shiny
Transparent colonies
Blue sheen
No haemolysis
S or R form
What are serological tests for Brucella and Myobacterium based on?
Detection of postinfection antibodies
Required during transport of animals
For prevention
What antigen does Brucella and Myobacterium have?
Somatic O antigen: A, M and R. Part of lipopolysaccharide-protein complex.
Name serological tests for blood
Agglutination (slide, tube)
Complement Fixation Test
ELISA
Serological test for milk
Milk ring test (slow tube agglutination)
Special test for Brucella (alergenodiagnostics)
Dermal test - application of F-allergen (abortin or brucelin) which causes inflammation and induration in 24-72 hours.
Used for alergenodiagnostics - late hypersensitivity reaction
