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Microbiology CT 4 > Brucellaceae > Flashcards

Brucellaceae Flashcards

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1
Q

Name selective staining method for Brucella

A

Stamp (positive = red, negative = green)

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2
Q

What type of pathogen are Brucella?

A

Obligate pathogens - cause zoonoses

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3
Q

Microscopical examination of Brucellaceae

A

Gram -
Small rods or coccobacilli
No specific arrangement
Non-motile
Acid-resistant

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4
Q

Brucella relation to oxygen

A

aerobic or microaerophilic

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5
Q

Brucella reaction on catalase, oxidase and urease

A

Catalase +, oxidase + and urease +

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6
Q

Brucella growth on common nutrient media

A

Weak

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7
Q

What kind of parasites are Brucellaea?

A

Intracellular parasites, able to multiply within the macrophages

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8
Q

Brucellaceae affinity to reproductive tract

A

Cause placentitis, abortus, infertility, orchitis and epididymitis

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9
Q

Virulence factors of Brucellaceae

A

Endotoxin
Somatic O-antigen
Intracellular parasitism

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10
Q

Important zoonotic species of Brucellaceae (5)

A

Brucella abortus
Brucella melitensis
Brucella suis
Brucella canis
Brucella neotomae

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11
Q

Bovine brucellosis is caused by:

A

Brucella abortus

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12
Q

Ovine and caprine brucellosis is caused by

A

Brucella melitensis

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13
Q

Brucellosis of swine and hares is caused by

A

Brucella suis

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14
Q

Canine brucellosis is caused by

A

Brucella canis

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15
Q

Brucellosis in rodents is caused by

A

Brucella neotomae

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16
Q

Which Brucella is NOT a zoonotic pathogen?

A

Brucella Ovis

17
Q

Brucella Ovis causes

A

infectious epididymitis of rams

18
Q

How is Brucella excreted?

A

In bovine milk

19
Q

Material for cultivation of Brucellaceae

A

fetus, lavage from uterus, vaginal swabs, lymph nodes, blood or milk

20
Q

Cultivation properties of Brucellaceae

A

S (or R) form
Aerobic, microaerophilic (5-10% CO2)
37 degrees Celsius (20-40C)
Incubation time is 3-5 days

21
Q

Cultivation media for Brucella

A

enriched media such as blood serum, tissue extract, vitamins or 1% glycerin

22
Q

Brucella on blood agar

A

Small
Shiny
Transparent colonies
Blue sheen
No haemolysis
S or R form

23
Q

What are serological tests for Brucella and Myobacterium based on?

A

Detection of postinfection antibodies
Required during transport of animals
For prevention

24
Q

What antigen does Brucella and Myobacterium have?

A

Somatic O antigen: A, M and R. Part of lipopolysaccharide-protein complex.

25
Q

Name serological tests for blood

A

Agglutination (slide, tube)
Complement Fixation Test
ELISA

26
Q

Serological test for milk

A

Milk ring test (slow tube agglutination)

27
Q

Special test for Brucella (alergenodiagnostics)

A

Dermal test - application of F-allergen (abortin or brucelin) which causes inflammation and induration in 24-72 hours.
Used for alergenodiagnostics - late hypersensitivity reaction

Microbiology CT 4 (6 decks)

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