Mycology Virology Flashcards

1
Q

Aerial hyphae of septate fungi

A

Conidiophore

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2
Q

Aerial hyphae of aseptate fungi

A

Sporangiophore

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3
Q

Sexual spores

A

“BAZO”
Basidospores
Ascospores
Zygospores
Oospores

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4
Q

Resting spores produced by rounding up and enlargement of terminal hyphal cells

A

Chlamydoconidia

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5
Q

Fragmentation of hyphae/mycelia

A

Arthroconidia

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6
Q

Zygomycetes

A

“RAM”
Rhizopus
Absidia
Mucor

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7
Q

Olive oil-loving fungi

A

Malassezia furfur
Tinea versicolor
Ptyriasis

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8
Q

Dermatophytes

A

“MET”
Microsporum
Epidermophyton
Trichophyton

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9
Q

Dermatophyte in man

A

Microsporum audounii

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10
Q

Favic chandelier

A

Trichiphyton shoenleinii
Trichophyton violaceum

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11
Q

Green-yellow fluorescence of ectothrix hair

A

M. canis

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12
Q

Apple-green fluorescence of ectothrix hair

A

M. audouinii

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13
Q

Large, club-shaped, smooth-walled macroconidia

A

Epidermophyton floccosum

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14
Q

Pencil-shaped macroconidia
Wine-red pigment

A

T. rubrum

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15
Q

Cigar-shaped macroconidia
Grape-like clusters microconidia
Spiral hyphae

A

T. mentagrophytes

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16
Q

“Balloon forms” microconidia
Requires thiamine

A

T. tonsurans

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17
Q

Rat-tail macroconidia

A

T. verrucosum

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18
Q

Flowerette conidia (mold)
Cigar-shaped (yeast)
Asteroid bodies (tissue)

Rose gardener’s disease

A

Sporothrix schenckii

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19
Q

North american blastomycosis

A

Blastomycoces dermatitidis

Gilchrist’s disease

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20
Q

South american blastomycoses

A

Paracoccidiodes brasiliensis

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21
Q

Single bud, broad base (yeast)
“Lollipop” (mold)

A

Blastomycoces dermatitidis

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22
Q

Multiple buds, ship/Mariner’s wheel, Mickey mouse (yeast)

A

Paracoccidiodes brasiliensis

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23
Q

Round to oval yeast, intracellular (yeast)
Tuberculated macroconidia (mold)
Darling’s disease

A

Histoplasma capsulatum

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24
Q

Mistaken as histoplasma

A

Amastigote form of Leishmania

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25
Q

Spherules with endospores (yeast)
Thick-walled barrel-shaped arthroconidia, empty disjunctor cells (mold)

A

Coccidiodes immitis

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26
Q

San Joaquin Valley Fever / Desert Fever

A

Coccidiodes immitis

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27
Q

Resembles myelin globules in sputum

A

Blastomyces

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27
Q

Resembles lymphocytes in CSF

A

Cryptococcus neoformans

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28
Q

Removed from SDA medium to grow C. neoformans

A

Cycloheximide

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28
Q

Test for systemic mycoses
Microscopic immunodiffusion

A

Exo antigen test

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28
Q

Cryptococcus neoformans identifying tests

A

Urease positive
Inositol positive
Nitrate negative

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29
Q

Stain in Aman’s medium

A

LPCB

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30
Q

Fungi color in acridine orange stain

A

Orange

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31
Q

Stains cellulose and chitin

A

Calcofluor white stain
Fungi under UV light: white to blue-green

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32
Q

Gram stain for fungi/yeast

A

Hucker’s modification (gram positive)

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33
Q

Best stain for fungi

A

Gomori methenamine silver (GMS)

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34
Q

Fungi culture are held for

A

21 days

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35
Q

SDA-CC

A

Mycosel or Mycobiotic
Sabouraud dextrose agar with Chloramphenicol and Cycloheximide

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36
Q

Enhances chlamydospores of Candida

A

Cornmeal agar

37
Q

Medium to differentiate T. rubrum form T. mentagrophytes

A

Cornmeal with 1% dextrose

38
Q

Czapek’s agar

A

Aspergillus

39
Q

Birdseed/Nigerseed/Staib’s medium

A

C. neoformans
Black colonies due to phenol oxidase

40
Q

Cottonseed medium

A

Blastomyces dermatitidis

41
Q

Hair baiting test positive

A

T. mentagrophytes

42
Q

BHI
Chromogenic
Dermatophyte test
Inhibitory mold
Potato flake
Mycosel
SABHI
Yeast-extract phosphate

A

Primary recovery media for fungi

43
Q

DNA virus replicate in the nucleus EXCEPT

A

Poxvirus (largest DNA virus)

44
Q

RNA virus replicate in the cytoplasm EXCEPT

A

Influenza, Retroviruses

45
Q

sDNA

A

Parvovirus

46
Q

dRNA

A

Reoviridae

47
Q

Culture cell for CMV

A

Human diploid fibroblast HDF

48
Q

Culture cell for influenza

A

Embryonated chicken eggs

49
Q

Cowdry type A

A

Influenza CPE

50
Q

Koilocytosis

A

HPV CPE

51
Q

Hemagglutination inhibition

A

for Rubella and Influenza antibodies

52
Q

For HSV and VZV detection from cutaneous vesicles

A

Tzanck smear

53
Q

Smallest DNA virus

A

Parvovirus

54
Q

DNA viruses

A

“Pa, Pa-Add Po He He”
Papilloma + Polyoma = Papova
Parvo
Adeno
Pox
Herpes
Hepadna

55
Q

Herpesviridae are cultured on

A

Rabbit kidney cells

56
Q

Positive PAP

A

Koilocytes
Heterogenous cytoplasm
Hyperchromatic nucleus

57
Q

Downey cells
Infectious mononucleosis
Kissing disease

A

EBV

58
Q

Heterophile-negative infectious mononucleosis
Owl’s eye inclusion CPE

A

CMV

59
Q

HHV dormant in T cells

A

HHV-6 and 7

60
Q

Roseola
Exanthem subitum
HHV-6 and 7

A

6th childhood disease

61
Q

HHV-8

A

Kaposi’s sarcoma

62
Q

DNA hybridization technique (molecular) for HPV

A

Hybrid capture assay

63
Q

PML Progressive multifocal leukoencephalopathy
BKV Hemorrhagic cystitis

A

Polyomavirus (Papovaviridae)

64
Q

Erythema infectiosum
Slapped cheek rash
5th childhood disease

A

Parvovirus B-19

65
Q

Variola major

A

Smallpox

66
Q

Variola minor

A

Alastrim

67
Q

Vaccinia virus

A

Cowpox

68
Q

Smallest RNA virus

A

Enterovirus (Picornaviridae)

69
Q

Largest RNA virus

A

Paramyxoviridae

70
Q

Naked RNA

A

Calici, Picorna, Reo

71
Q

Arboviruses

A

Bunya, Toga, Flavi

72
Q

Arboviruses causes

A

Fever and encephalitis

73
Q

BSL-4 organisms causes

A

Hemorrhagic fever

74
Q

LCM Lymphocytic choriomeningitis
Lassa fever
Machupo virus

A

Arenaviridae

75
Q

California encephalitis group
LaCrosse virus
Hanta virus

A

Bunyaviridae

76
Q

Most common cause of diarrhea in adults

A

Norwalk

77
Q

Norwalk
Hepatitis E

A

Caliciviridae

78
Q

High case fatality in pregnant women

A

Hepatitis E virus

79
Q

Ebola
Marburg virus

A

Filoviridae

80
Q

Arboviruses (Dengue, Japanese encephalitis, St. Louis, West Nile

A

Flaviviridae

81
Q

Molecular gold standard of strain typing

A

Pulse Field Gel Electrophoresis PFGE

82
Q

Hand-foot-mouth disease

A

Coxsackie A virus

83
Q

Acid resistant enterovirus
Acid sensitive enterovirus

A

Enterovirus
Rhinovirus

84
Q

Foot and mouth disease

A

Aphthovirus

85
Q

ECHO virus

A

Enteric cytopathic human orphan

86
Q

REOviridae

A

Respiratory enteric orphan

87
Q

Winter diarrhea
Gastroenteritis in children

A

Rotavirus (Reoviridae)

88
Q

Retroviridae

A

HIV
HTLV

89
Q

Picornaviridae

A

Enteroviruses:
Polio, Coxsackie, Echo, Entero
Hepatitis A
Rhinovirus

90
Q

Rubella virus
Arbovirus

A

Togaviridae

91
Q

Mild exanthematous disease

A

Rubella

92
Q

Congenital rubella

A

German measles (Togaviridae)

93
Q

Photophobia

A

Chikungunya