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Microbiology 2 Flashcards

Staph, Strep, Neisseria, Moraxella

1
Q

Cause of meningitis for neonates and immunocompromised

A

S. agalactiae (most common)
E. coli
Listeria

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2
Q

Cause of meningitis for infants (1-23 months)

A

H. influenza b

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3
Q

Cause of meningitis for 5-29 years old

A

N. meningitidis

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4
Q

Localized scalded skin syndrome

A

Pemphigus neonatorum/Bullous impetigo

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5
Q

SAU toxin that causes SSS

A

Exfoliative/Epidermolytic toxin

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6
Q

SAU cytotoxin that is toxic to WBCs

A

Panton-Valentin-Leukocidin (gamma toxin)

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7
Q

Hyaluronidase

A

Duran-Reynal Factor / Spreading factor

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8
Q

Bacterial endocarditis following prosthetic heart valve insertion

A

Staph. epidermidis

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9
Q

Slime-producing staph

A

S. epidermidis, S. aureus

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10
Q

Selective/differential medium for MRSA

A

CHROMagar (Alain Rambach)
contains cefoxitin

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11
Q

SAU colonies on CHROMagar

A

mauve-colored

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12
Q

Coagulase test reagent

A

Rabbit’s plasma (EDTA)

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13
Q

DNAse test positive organisms

A

“SSS Mo”
S. aureus S. pyogenes Serratia
Moraxella

“DNA green yung MoSSS”
(+) colorless
(-) remains green

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14
Q

“Dog bite infection”
VP negative

A

Staph. intermedius (PYR+) “inter PYR”
Staph. hyicus

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15
Q

“Porcelain white” in LSS

A

S. albus (S. epidermidis)

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16
Q

Novobiocin susceptible/resistant

A

S. epidermidis ≥16mm
S. saprophyticus

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17
Q

“Foam-loving” 10% CO2 foam (HACEK)

A

Aggregatibacter (Haemophilus) aprophilus

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18
Q

Tear drop forms (HACEK)

A

Cardiobacterium hominis

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19
Q

S. pyo M protein is antiphagocytic because

A

it binds to beta globulin FACTOR H

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20
Q

TSST-1

A

Enterotoxin F

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21
Q

Strep pyrogenic exotoxin A & C causes

A

Scarlet fever
Streptococcal Toxic shock-like syndrome

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22
Q

Erysipelas causative agent

A

S. pyogenes

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23
Q

Erysipeloid causative agent

A

E. rhusiopathiae

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24
Q

Erythrasma causative agent

A

Corynebacterium minutissimum

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25
Q

Scarlet fever (susceptibility test)

A

Dick’s test

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26
Q

Schick’s test

A

Diptheria

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27
Q

Scarlet fever (diagnostic test)

A

Schultz-Charlton test

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28
Q

Post-streptococcal sequelae

A

Rheumatic heart fever
AGN

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29
Q

Multi-drug resistant (MDR) organism (Enterococci) causes

A

Nosocomial infections:
UTI, bacteremia, endocarditis, pneumonia

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30
Q

Upper respiratory tract/oral normal flora

A

Viridans strep
HACEK

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31
Q

Subacute bacterial endocarditis (in damaged heart valves) gram positive causative agent

A

Viridans strep

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32
Q

Subacute bacterial endocarditis gram negative causative agent

A

HACEK

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33
Q

Lobar/community-acquired pneumonia, rust-colored sputum

A

S. pneumoniae

34
Q

Currant jelly sputum

A

K. pneumoniae

35
Q

Bullet/Lancet/Flame-shaped bacteria

A

S. pneumoniae

36
Q

Bullet-shaped virus

A

Rhabdoviridae (Rabies)

37
Q

Bile solubility test reagent (tube)

A

2% sodium desoxycholate

38
Q

Skin test for pneumococcal antibodies

A

Francis test

39
Q

PYR substrate

A

L-pyrrolidonyl-B-naphthylamide

40
Q

PYR reagent & positive result

A

PDAC
p-dimethylaminocinnamaldehyde
(+) cherry red

41
Q

HH reagent & positive result

A

Ninhydrin reagent (detects glycine)
(+) purple/deep blue

42
Q

(+) CAMP, HH

A

Group B Strep (S. agalactiae)

43
Q

Group D Enterococcus vs Non-enterococcus

A

(+)PYR, 6.5% NaCl, 10C growth on nutrient agar
Penicillin resistance

44
Q

Taxo A

A

Group A Strep
Bacitracin >10mm

45
Q

Taxo P

A

S. pneumoniae
Optochin >14mm

46
Q

Taxo P chemical name

A

Ethylhydrocupreine HCl

47
Q

Differentiate Strep. anginosus from other beta-hemolytic strep

A

VP positive

48
Q

Viridans strep causing gastrointestinal malignany (cancer)

A

S. bovis

49
Q

Nutritionally-variant streptococci

A

Satelliting-streptococci, thiol-requiring (cysteine, pyridoxal)
Abiotrophia & Granulicatella spp.

50
Q

(+) Satellitism in Staph streak test

A

H. influenzae
“dew drop colonies”

51
Q

CPOP

A
  1. Micrococcus
  2. Neisseria, Haemophilus
  3. Plesiomonas, Aeromonas, Vibrio
  4. Oxidizer: Pseudomonas, Burkholderia
52
Q

CPON

A
  1. Staphylococcus
  2. All Enterobacteriaceae except Plesiomonas
  3. Francisella
  4. Oxidizer: Stenotrophomonas, Acinetobacter
53
Q

Aerobic gram negative cocci

A

Neisseria, Moraxella

54
Q

Anaerobic gram negative cocci

A

Veilonella

55
Q

Endotoxin triad of symptoms

A

Fever, DIC, septic shock

56
Q

Has IgA protease as virulent factor

A

Meningitis causative agents:
Neisseria
Haemophilus
S. pneumoniae

57
Q

Disseminated gonococcal infections

A

Pelvic inflammatory disease
Fitz-Hugh-Curtis syndrome (peritoneum)
Arthritis

58
Q

Gram neg cocci causing DIC, Waterhouse-Friderichsen syndrome

A

N. meningitidis

59
Q

N. meningitidis capsule associated with community-acquired meningitis

A

Polysaccharide capsule B

60
Q

Common cause of otitis media

A

Moraxella catarrhalis

61
Q

Sensitive to cotton swab

A

N. gonorrhoeae
B. pertussis

62
Q

A male is diagnostic for gonorrhoea if

A

Positive smear with symptoms

63
Q

Diagnostic procedure for non-toxin-producing organisms

A

Culture

64
Q

Incubation of Neisseria

A

35C in 3-5% CO2 for 3 days (72 hours)

65
Q

Selective medium for pathogenic Neisseria

A

TMA

66
Q

Thayer Martin inhibitors

A

Vancomycin (gram +)
Colistin (gram -)
Nystatin (fungi/yeast)

67
Q

Modified Thayer Martin

A

TMA with Trimethoprim lactate (swarming Proteus)

68
Q

Martin Lewis fungi/yeast inhibitor

A

Anisomycin
“MLA MYC Manila, my city”

69
Q

New York City Agar fungi/yeast inhibitor

A

Amphotericin B
“AmB American boy, New York”

70
Q

GC-LECT

A

New York City agar with LINCOMYCIN

71
Q

Selective media for N. gonorrheae that uses LYSED HORSE blood

A

NYCA

72
Q

Smooth, glistening, claylike colonies (BAP)

A

Neisseria elongata

73
Q

Colony may be moved intact over agar surface

A

M. catarrhalis (“hockey puck”)
H. ducreyi

74
Q

Catalase negative, nondiplococci Neisseria

A

N. elongata (rod-shaped)

75
Q

Also grow in TMA

A

Acinetobacter
Capnocytophaga
Kingella

76
Q

Taxo N positive result

A

Oxidase test
(+) purple - Neisseria, Moraxella

77
Q

Superoxol test reagent & positive result

A

30% H2O2
(+) vigorous bubbling - N. gonorrhoeae

78
Q

Asaccharolytic bacteria

A

Acinetobacter iwoffi
Moraxella
Oligella
Alcaligenes

79
Q

Moraxella catarrhalis identifying tests

A

DNAse positive
Butyrate esterase positive
Asaccharolytic

80
Q

N. meningitidis subtypes

A

A, B, Y, W-135

81
Q

Specimen for NAAT of N. gonorrhoeae and Chlamydia

A

Urethral swab, urine

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