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Microbiology 3 Flashcards

Enterobacteriaceae

1
Q

Nonmotile Enterobacteriaceae

A

Shigella Klebsiella
Y. enterocolitica @37C

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2
Q

Nonmotile @ 37C

A

Y. enterocolitica
Listeria

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3
Q

Biochemically inert

A

Shigella - only Methyl red (+)

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4
Q

“CPON NO GF FA”

A

Enterobacteriaceae

Catalase positive Oxidase negative
Nitrate reducers
Glucose fermenters
Facultative anaerobes

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5
Q

LF enzymes

A

Lactose permease
Beta-galactosidase

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6
Q

RLF + TSI reaction

A

“EKE”
Escherichia Klebsiella Enterobacter
TSI: A/A H2S(-)

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7
Q

LLF + TSI reaction

A

“Cit Ser, Yer Haf Sa Shi is LATE”
Citrobacter
Serratia
Yersinia enterocolitica
Hafnia
Salmonella arizonae
Shigella sonnei

TSI: A or K/A

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8
Q

NLF + TSI reaction

A

“Sarap-Sarap ng PPM Eh!”
Salmonella
Shigella
Proteus
Providencia
Morganella
Edwardsiella

TSI: K/A

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9
Q

Rapid urease producer except

A

Providencia stuartii

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10
Q

PPM

A

Rapid urease producer
LIA: R/A (lysine deamination)
Phenylalanine deaminase (+)
Motile
UTI

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11
Q

TSI K/K

A

Pseudomonas

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12
Q

Most common cause of UTI in human

A

Uropathogenic E. coli (UPEC)

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13
Q

ETEC stool

A

Profuse watery stool
Traveler’s diarrhea/Montezuma’s revenge

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14
Q

Cholera-like toxin

A

ETEC
Heat-labile enterotoxin

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15
Q

Shiga-like toxin

A

EHEC (Verotoxin)

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16
Q

Shigella-like infection

A

EIEC

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17
Q

EIEC stool

A

Explosive watery stool with mucus and blood

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18
Q

EIEC strains

A

Like Shigella: nonmotile, NLF

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19
Q

Shigella stool

A

Scanty, bloody

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20
Q

Watery diarrhea with mucus, no blood (non-invasive, no toxin)
Infantile diarrhea/pediatrics

A

EPEC

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21
Q

Green metallic sheen on EMB

A

E. coli

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22
Q

Associated with E. coli O157:H7

A

EHEC/VTEC

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23
Q

Sorbitol (-) Colorless on SMac
MUG (-)

A

E. coli O157:H7

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24
Q

Most severe manifestation of EHEC

A

HUS

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25
Q

Jack in the Box outbreak - beef hamburger disease
Hemorrhagic colitis
HUS

A

E. coli O157:H7

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26
Q

Adhere to HEp2 in aggregative “stacked brick pattern”

A

EAEC

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27
Q

Indole positive Klebsiella

A

K. oxytoca + - ++

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28
Q

LDC (+)

A

Klebsiella, Salmonella

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29
Q

Enterobacter slow urease (+)

A

E. gergoviae

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30
Q

Enterobacter produce yellow pigmentation that intensifies at 25C

A

Enterobacter sakazakii

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31
Q

Slow urease producers

A

“CKEYS”
Citrobacter
Klebsiella
Enterobacter gergoviae
Y. enterocolitica
Serratia

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32
Q

Triple LOA (-)

A

Pantoea agglomerans

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33
Q

Triple LOA (+)

A

Plesiomonas

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34
Q

Triple enzyme (+)

A

DNAse, Lipase, Gelatinase (+)
Serratia

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35
Q

E. aerogenes LOA

A

++-

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36
Q

E. cloacae LOA

A

-++

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37
Q

K. pneumoniae LOA

A

+–

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38
Q

E. coli LOA

A

++-

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39
Q

Donovan bodies (pathognomonic)
Granuloma inguinale/donovanosis

A

Klebsiella granulomatis

40
Q

Produce prodigiosin (red pigment) at room temp

A

Serratia marcescens

41
Q

NLF H2S(+) Motile LDC (+)

A

Salmonella

42
Q

Pea soup stool (green)

A

Salmonella

43
Q

(+) Methyl red, ONPG
Slow urease (+)
Growth on KCN agar

A

Citrobacter freundii

44
Q

Selective medium for S. typhi

A

Bismuth Sulfite Agar BSA
Black colonies with metallic sheen

45
Q

Milk-borne disease of cow

A

S. typhi

46
Q

OX-K source

A

P. mirabilis

47
Q

Shigella ONPG+

A

S. sonnei

48
Q

Associated with staghorn/struvite/Mg ammonium phosphate stones/triple phosphate crystal

A

Proteus

49
Q

H2S (+)

A

“SPACEd”
Salmonella
Proteus
Arizonae
Citrobacter freundii
Edwardsiella tarda

50
Q

Providencia differentiating test

A

Citrate (+)

51
Q

Morganella differentiating test

A

ODC (+)

52
Q

E. tarda differentiating test from E. coli (same IMViC)

A

H2S+
NLF

53
Q

Vector of Y. pestis

A

Xenopsylla cheopsis (rat flea)

54
Q

Cauliflower colonies on BAP 48H

A

Y. pestis

55
Q

Appendicitis-like pain in children is caused by

A

Y. enterocolitica

56
Q

Stalactite pattern in broth culture

A

Y. pestis

57
Q

Bull’s eye colonies in CIN after 48H

A

Y. enterocolitica
Aeromonas

58
Q

Preferential growth temp of Yersinia

A

25-30C

59
Q

Motile at 25C, nonmotile at 37C

A

Yersinia

60
Q

Sulfur source of H2S medium

A

Sodium thiosulfate

61
Q

Sulfur source of TSI

A

Ferrous sulfate (nonmetallic)

62
Q

Other H2S media and their H2S indicator

A

SSA XLD HEA
SIM LIA

Ferric ammonium citrate (FAC)

63
Q

Media with phenol red as indicator

A

“MX CUT”
MSA
XLD
CTA
Urease
TSI

64
Q

NLF nonH2S producer

A

“SSPPM minus SPACEd”
Shigella
Providencia
Morganella

65
Q

Lysine deaminase positive
Phenylalanine deaminase positive

A

PPM

66
Q

Product of deamination of acids

A

Ammonia

67
Q

K/A H2S-

A

Shigella

68
Q

Tests that uses bromthymol blue as indicator

A

Citrate, Acetate, Acetamide, Malonate

69
Q

Fish-eye colonies on EMB (pink with dark center)

A

Enterobacter

70
Q

Pink colonies with pink halo on MAC

A

E. coli

70
Q

XLD inhibitor

A

Sodium desoxycholate

71
Q

Yellow colonies with black center on XLD

A

Proteus

72
Q

E. coli on HEA

A

Salmon orange

73
Q

E. coli on XLD

A

Yellow with yellow halo

74
Q

Indole test detects

A

Tryptophanase

75
Q

Indole test indicator

A

PDAB Kovac’s/Ehrlich’s reagent

76
Q

Indole positive control

A

E. coli

77
Q

Spot indole test reagent and positive result

A

PDAC
(+) blue

78
Q

Indole positive Enterobacteriaceae

A

“May PEKPEC Po?”
Morganella
Proteus vulgaris
E. coli
Klebsiella oxytoca
Edwardsiella tarda
Citrobacter koseri
Plesiomonas

79
Q

Methyl red test positive end products are

A

“LAFS” mixed acid fermentation pathway
Lactic acid
Acetic acid
Formic acid
Succinic acid

80
Q

VP reagents

A

a-naphthol and 40% KOH

81
Q

VP detects

A

acetoin

82
Q

VP positive

A

“KEESH”
Klebsiella
Enterobacter
Ewingella
Serratia
Hafnia

83
Q

Media with bromthymol blue indicator

A

“CHOT”
Citrate
HEA
OF Hugh Leifson
TCBS

84
Q

Citrate positive

A

“PeRO Sa CHEEKS”
Providencia
Serratia
Citrobacter
Hafnia
Ewingella
Enterobacter
Klebsiella
Salmonella enteritidis

85
Q

Rapid urease producers

A

“TPUNCH”
T. mentagrophytes
PPM
Ureaplasma
Nocardia
C. neoformans
H. pylori

86
Q

LOA end products

A

“Let’s Order A CaPuCino”
Lysine - Cadaverine
Ornithine - Putrescine
Arginine - Citrulline

87
Q

Medium where all Salmonella will grow except S. typhii

A

Brilliant green agar

88
Q

Shigella group types

A

“DA FB BC SD”
S. dysenteriae - A
S. flexneri - B
S. boydii - C
S. sonnei - D

89
Q

Indole negative proteus

A

Proteus mirabilis

90
Q

OX-2 and 19 source

A

P. vulgaris

91
Q

Cold enrichment
Non motile at 37C

A

Y. enterocolitica

92
Q

Most common cause of death following transfusion of packed red cells

A

Y. enterocolitica

93
Q

CPON

A
  1. Staphylococcus
  2. Enterobacteriaceae
  3. Francisella
  4. Oxidizer: Stenotrophomonas, Acinetobacter
94
Q

CPOP

A
  1. Micrococcus
  2. Neisseria, Haemophilus
  3. Plesiomonas, Aeromonas, Vibrio
  4. Oxidizer: Pseudomonas, Burkholderia
  5. Campylobacter, Helicobacter, Chromobacterium

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