Mycology Flashcards
fungi are gram
positive
colonies are moist creamy, opaque, pasty
yeast
yeast grows at
37 deg C
unicellular and exist as monomorphic
a. yeast
b. mold
a
multicellular and exist dimorphic
a. mold
b. yeast
a
mold grows at
RT; 25-30 degC
what do you call a group of hyphae
mycelia
this is used for reproduction and is extended at the surface
aerial
part that is used for nutrition
phallus / vegetation
causes subcutaneous mycoses
a. sporothrix schenckii
b. histoplasma capsulatum
c. blastomyces dermitidis
d. coccidiodes immitis
a
causes systemic mycoses
C- capsulatum
D- dermitidis
I- immitis
B- braziliense
basic structural unit of fungi and also known as —-
hyphae; molds
aseptate hyphae
zygomycetes; Mucor, Absidia, Rhizor
with pectinate body
a. m. audouinii
b. microsporum canis
c. t. schoenleinii - swollen
d. epidermophyton flocossum
a
with antler hyphae
a. m. audouinii
b. microsporum canis
c. t. schoenleinii - swollen
d. epidermophyton flocossum
c
with racquet hyphae
a. m. audouinii
b. microsporum canis
c. t. schoenleinii - swollen
d. epidermophyton flocossum
d
spiral hyphae
a. trichophyton mentagrophytes
b. microsporum canis
c. t. schoenleinii
d. epidermophyton flocossum
a
with nodular bodies
a. m. audouinii
b. microsporum canis
c. t. schoenleinii - swollen
d. epidermophyton flocossum
b
dematiaceous
pigmentation due to melanin
non-pigmented
hyaline cast
structures for reproduction
spores
spores are formed as a result of nuclear fusion
a. asexual spores/ imperfect
b. sexual spores/ perfect
b
spores are formed without nuclear fusion
a. asexual spores/ imperfect
b. sexual spores/ perfect
a
asexual spores
a. teleomorph
b. anamorph
c. ascospores
d. basidiospores
b
sexual spores
a. teleomorph
b. anamorph
c. blastoconidia
d. chamydoconida
a
formed thru budding - small outgrowth of hyphae pinches off
a. ascospores
b. blastoconidia
c. chamydoconida
d. zygospores
b
formed thru fragmentation of hyphal cells
a. arthroconidia
b. blastoconidia
c. chamydoconida
d. zygospores
a
formed thru enlargement of hyphal cells
a. ascospores
b. blastoconidia
c. chamydoconida
d. zygospores
c
formed within a SAC like structure called ASCUS
a. ascospores
b. blastoconidia
c. chamydoconida
d. zygospores
a
formed within a CLUB shaped structure called BASIDIUM
a. ascospores
b. blastoconidia
c. chamydoconida
d. basidiospores
d
formed thru the union of 2 undifferentiated cells
a. oospores
b. zygospores
c. chamydoconida
d. zygospores
b
formed thru the union of 2 differentiated cells
a. ascospores
b. blastoconidia
c. oospores
d. zygospores
c
pyriform-shaped
a. microconidia
b. macroconidia
a
spindle-shaped
a. microconidia
b. macroconidia
b
temporary mount
KOH; 10-20 percent
lactophenol cotton blue
india ink/nigrosin
calcofluor white stain
detects fungal elements from skin, hair and nails
a. KOH; 10-20 percent
b. lactophenol cotton blue
c. india ink/nigrosin
d. calcofluor white stain
a
for preservative and stains
a. KOH; 10-20 percent
b. lactophenol cotton blue
c. india ink/nigrosin
d. calcofluor white stain
b
to detect c. neoformans CAPSULE
a. KOH; 10-20 percent
b. lactophenol cotton blue
c. india ink/nigrosin
d. calcofluor white stain
c
to demonstrate chitin on cell wall
a. KOH; 10-20 percent
b. lactophenol cotton blue
c. india ink/nigrosin
d. calcofluor white stain
d
permanent mounts
Periodic acid schiff and gomori’s methenamine silver
Mayer’s mucicarmine
Fontana masson ; H and E
Giemsa or Wrights
Gram’s stain or acid fast kinyoun’s
detect fungi in histologic sections
a. Periodic acid schiff and gomori’s methenamine silver
b. Mayer’s mucicarmine
c. Fontana masson ; H and E
d. Giemsa or Wrights
a
demonstration of C. neoformans
a. Fontana masson ; H and E
b. Mayer’s mucicarmine
c. Periodic acid schiff and gomori’s methenamine silver
d. Giemsa or Wrights
b
detects hyphal pigmentation
a. Fontana masson ; H and E
b. Mayer’s mucicarmine
c. Periodic acid schiff and gomori’s methenamine silver
d. Giemsa or Wrights
a
detects yeast within mononuclear cells i.e., Histoplasma capsulatum
a. Fontana masson ; H and E
b. Mayer’s mucicarmine
c. Periodic acid schiff and gomori’s methenamine silver
d. Giemsa or Wrights
d
demonstration of fluorescens of fungal elements usually on hair
a. wood’s lamp
b. culture media
c. incubation temperature for fungi
d. antimicrobial agents in fungal media
a
incubation temperature for fungi
25-30 degC or RT
most fungi grow within —–
2 weeks incubation period
antimicrobial agents in fungal media
chloramphenicol and cyclohexidine
to prevent bacterial growth
a. chloramphenicol
b. cycloheximide
a
to prevent saprophytic fungi
a. chloramphenicol
b. cycloheximide
b
czapek agar is used to detect
a. c. neoformans
b. used as nutrient media to detect fungi
c. aspergillus
d. mycosel/mycobiotic
c
dermatophytes can be detected by using
a. c. neoformans
b. cornmeal agar
c. aspergillus
d. mycosel/mycobiotic
d
cornmeal agar is used for
a. detect c. neoformans
b. stimulate chlamydospore formation of C.albicans
c. aspergillus
d. mycosel/mycobiotic
b
nutrient media for fungi
a. c. neoformans
b. cornmeal agar
c. saboraud’s dextrose agar
d. bird seed, niger seed, staib’s medium
c
what culture media is being used for C. neoformans
a. aspergillus
b. czapek agar
c. saboraud’s dextrose agar
d. bird seed, niger seed, staib’s medium
d
tests that will differentiate t. mentagrophytes and t. rubrum
urease test and hair baiting/perforation
positive result in urease test
pink to red
positive result in hair baiting/perforation
v-shaped or conical hair perforation
t. mentagrophytes’ result in hair baiting and urease test
positive
t. mentagrophytes’ result in hair baiting and urease test
negative
presumptive test for candida albicans
a. wood’s lamp
b. culture media
c. urease test
d. germ tube test
d
germ tube test procedure
small inoculum of yeast in 0.5 mL sheep’s serum or rabbit’s plasma then incubate at 37-37 degC no longer than 3 hrs.
in germ tube test this will indicate positive result
NO CONSTRICTION
in germ tube test positive result may be mistaken from ——- however it possess ——
hyphae; called as pseudohyphae, possesses constriction
this is used for rapid identification of c. neoformans
a. wood’s lamp
b. L-dopa ferric citrate test
c. urease test
d. germ tube test
b
positive result in L-dopa ferric citrate test
black
this causes tinea or pityriasis versicolor
m. furfur
form of mycosis characterized by depigmentation/discoloration of skin
superficial mycosis
a lipophilic yeast
m. furfur
m. furfur can be describe as —-, what is its mode of transmission
spag with meatballs, direct contact
classify mycotic infections according to geographical distributions
superficial mycoses
example of superficial mycoses
tinea versicolor
tinea nigra
piedra - white and black
group of intertwined hyphae is called
mycelia/mycellium
plates/tubes are incubated at
ambient air
chronic superficial fungal infection which leads to hypopigmentation/hyper patches on skin
a. pityriasis versicolor
b. piedra
c. tinea nigra
a