mueller electron transport Flashcards
What is the lipid and protein composition of the outer mitochondrial membrane?
50% lipid and 50% protein
What is the protein and lipid composition of the inner membrane?
80% protein and 20% lipid
What is the one TCA cycle enzyme that is not soluble?
succinate dehydrogenase
What proteins do mitochondrial genes encode?
ATP synthase. Complexes I, III, IV
What are the reduced substrates for ETC?
NADH and succinate
What are the major dehydrogenases that funnel electrons to the ETC?
- succinate dehydrogenase
- NADH dehydrogenase
- fatty acyl-CoA dehydrogenase
- Glycerol-3-phosphate dehydrogenase
What is different about the cytochromes iron compared to hemeglobin or myoglobin?
cytochrome iron can undergo changes in its ionization state
What dehydrogenase contain FAD?
succinate, fatty acyl and glycerol-3-phosphate
WHat dehydrogenases contain FMN?
NADH
What is the purpose of CoQ?
shuttle electrons from various dehydrogenaes to complex III
What are complex I and II?
I. NADH - CoQ reductase
II. Succinate CoQ reductase
What reaction to complex II catalyze?
succinate to fumarate
What are complex III and IV?
III. Cytochrome c reductase
IV. cytochrome c oxygenase
What are two important inhibitors of complex IV?
cyanide and CO
What other metal ion does complex IV contain?
copper
What complex does not produce enough energy by itself to produce ATP?
complex II
What are the three sites of proton extrusion in ETC?
Complexes I, III, IV
How many ATP per NADH?
3 ATP
How many ATP per FADH2
2 ATP
What is the role of Fo?
transmembrane protein that forms a proton channel
What is F1?
catalytic moiety that is involved in the actual synthesis of ATP from ADP.
How does 2,4-DNP uncouple ETS?
by physically carrying protons from the periplasmic space and back into the mitochondrial matrix
How does ATP leave matrix?
via adenine nucleotide translocase
How does atractyloside inhibit electron flow?
via inhibition of adenine nucleotide translocase
