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HISTOPATH LEC > MOUNTING MEDIUM (GREGORIOS') > Flashcards

MOUNTING MEDIUM (GREGORIOS') Flashcards

1
Q

What happens to unmounted stained sections if viewed in the microscope?

A

Differences in LIGHT REFRACTION between the glass slides and the tissue components

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2
Q

The differences in LIGHT REFRACTION between the glass slides and the tissue components seen in unmounted stained sections may lead to what?

A

may lead to difference in LENGTH DISPERSION

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3
Q

The differences in LIGHT REFRACTION between the glass slides and the tissue components seen in unmounted stained sections may lead to what?

A

may lead to difference in LENGTH DISPERSION

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4
Q

Difference in length results in what?

A

results to LITTLE MICROSCOPIC DETAIL that can be appreciated

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5
Q

Tissues should be impregnated with a what?

A

with a transparent medium

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6
Q

Tissues should be impregnated with a transparent medium that has a what?

A

has an index of refraction close to that of the GLASS and the TISSUE

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7
Q

Tissues should be impregnated with a transparent medium that has a what?

A

has an index of refraction close to that of the GLASS and the TISSUE

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8
Q

The mounting medium bonds specimen, slide and coverslip together with a what?

A

with a clear DURABLE FILM

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9
Q

What does the mounting medium do to the specimen, slide and coverslip?

A

the mounting medium bonds the specimen, slide and coverslip together

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10
Q

What does the mounting medium do to the specimen, slide and coverslip?

A

the mounting medium bonds the specimen, slide and coverslip together

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11
Q

It is usually a syrupy fluid applied between the section and the coverslip AFTER staining

A

Mounting medium

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12
Q

The mounting medium is usually a syrupy fluid applied between what and when is it applied?

A

between the section and the coverslip AFTER staining

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13
Q

When the mounting medium is applied, what is its primary role?

A

it sets the section firmly preventing the movement of the COVERSLIP

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14
Q

The mounting medium prevents the movement of what?

A

movement of the COVERSLIP

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15
Q

It protects the stained section from getting SCRATCHED

A

mounting medium

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16
Q

it is used to facilitate easy handling and storage

A

mounting medium

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17
Q

it preserves the slides for permanent keeping

A

mounting medium

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18
Q

It helps in preventing the distortion of image during MICROscopic examination

A

mounting medium

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19
Q

Mounting mediua are often chosen for what?

A

for a specific refractive index

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20
Q

This enhances specimen details or make them invisible

A

The specific refractive index

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21
Q

What material becomes totally invisible if immersed in a solution of same refractive index?

A

Glass

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22
Q

When does materials like glass become totally invisible?

A

if immersed in a solution of the SAME REFRACTIVE INDEX

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23
Q

When does materials like glass become totally invisible?

A

if immersed in a solution of the SAME REFRACTIVE INDEX

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24
Q

It governs the contrast between the cellular detail and the background

A

Refractive index

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25
Q

It governs the transparency of the observed sample againt the BRIGHT FIELD of the microscope

A

Refractive index

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26
Q

The refractive index governs the contrast between what?

A

between the cellular detail and the background

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27
Q

The refractive index governs the transparency of what?

A

transparency of the OBSERVED SAMPLE against the BRIGHT FIELD of the microsope

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28
Q

What is the refractive index rule that the mounting media MUST ALWAYS HAVE?

A

The mounting media must always have an RI HIGHER than the mounted sample

29
Q

What is the refractive index rule that the mounting media MUST ALWAYS HAVE?

A

The mounting media must always have an RI HIGHER than the mounted sample

30
Q

What is the purpose of having a mounting media that has a RI higher than the mounted sample?

A

to impart MORE TRANSPARENCY

31
Q

This is taken from the last xylene bath with forceps

A

The SLIDE carrying the SECTION to be MOUNTED

32
Q

The slide carrying the section to be mounted is taken from what sequence using what?

A

taken from the LAST XYLENE BATH with FORCEPS

33
Q

This is wiped off from the back of the slide and from around the section

A

Excess xylene

34
Q

Excess xyelene is wiped off from what parts?

A

wiped off from the BACK of the slide and from AROUND the section

35
Q

A drop of mounting medium is placed at what position of the slide?

A

placed DOWN THE CENTER of the slide

36
Q

A clean, dry cover glass is placed on the EDGE of the slide and gradually inclined upward until it touches the mounting medium and gently pressed on to the slide while the mounting medium slowly spreads through the whole area of the slide.

True or False

A

inclined downward; quickly spreads; whole area of the section

FALSE

37
Q

A clean, dry cover glass is placed on the EDGE of the slide and gradually inclined upward until it touches the mounting medium and gently pressed on to the slide while the mounting medium slowly spreads through the whole area of the slide.

True or False

A

inclined downward; quickly spreads; whole area of the section

FALSE

38
Q

At what temperature and at how many hours may the slide be INCUBATED AFTER MOUNTING?

A

37 degC for 12-24 hours

39
Q

What is the purpose of incubating the slide at 37 degC for 12-24 hours after mounting?

A

to harden the MEDIUM

40
Q

When may the slide be incubated at 37 degC for 12-24 hours in order to harden the medium?

A

AFTER mounting

41
Q

DO NOT use immersion oil on an uncovered slide

true or false

A

TRUE

42
Q

What must NOT be used on an uncovered slide?

A

immersion oil

43
Q

What must be done if in a hurry to view a specimen under oil immersion?

A
  1. mount the slip
  2. handle the mount carefully
  3. keep it horizontal
44
Q

If in a hurry to view a specimen under oil immersion, oil can be applied, but do not attempt to wipe it off until the mounting medium is cured

True or False

A

TRUE

45
Q

At least how long until the mounting medium is cured in order to wipe off the oil?

A

at least overnight or an hour

46
Q

What is used at least overnight or for an hour to cure the mounting medium?

A

a hotplate or in an overn

47
Q

Store mounted slides vertically for 2 days if cured at room temperature

True or False

A

DO NOT store mounted slides vertically for 2 days if cured at RT

FALSE

48
Q

Store mounted slides vertically for 2 days if cured at room temperature

True or False

A

DO NOT store mounted slides vertically for 2 days if cured at RT

FALSE

49
Q

What happens if there is EXCESSIVE MOUNTING MEDIUM?

A
  • it will ooze out of the sides of the cover glass
  • it should be carefully wiped with a FINE cloth moistened with XYLENE
50
Q

What happens if there is EXCESSIVE BLOTTING?

A
  • It will dry up the section
  • will cause shrinkage and cracking of the specimen
51
Q

What must be done if the section has to be REMOUNTED?

A
  • The cover glass may be removed by soaking in XYLENE
52
Q

What will happen if the EXCESS XYLENE is not removed?

A
  • it will mix with the MOUNTANT
  • it will form BUBBLES on the SLIDE
53
Q

What will be the result if there is TOO LITTLE MOUNTING MEDIUM?

A
  • may cause IMPROPER SETTING of the COVERSLIP
  • formation of BUBBLES on the SECTION
54
Q

What will be the result if there is TOO LITTLE MOUNTING MEDIUM?

A
  • may cause IMPROPER SETTING of the COVERSLIP
  • formation of BUBBLES on the SECTION
55
Q

When there is formation of bubbles due to too little mounting medium, how can this be treated?

A

tease out by gently pressing on the COVER GLASS with POINTED FORCEPS or MOUNTING NEEDLE

56
Q

Setting may be hastened in a what?

A

in a HOT OVEN

57
Q

Setting may be hastened in a hot oven at what temperature for how long?

A

at 50 degC for 2 hours

58
Q

Basic aniline dyes should be mounted in non-acid containing mountants

True or False

A

TRUE

59
Q

Preparations showing the Prussian blue reaction should be mounted in a reducing media

True or False

A

non-reducing media

FALSE

60
Q

Preparations showing the Prussian blue reaction should be mounted in a reducing media

True or False

A

non-reducing media

FALSE

61
Q

Where is a mounting medium usually dispensed from and dispensed into?

A

dispensed from the STOCK BOTTLES into screw cap COLLAPSIBLE TIN TUBES

62
Q

What are the purposes of using a screw cap collapsible tin tube?

A
  • will keep the mountant clean
  • will prevent the concentration and thickening of the solution subsequent to EVAPORATION
63
Q

Slides should be properly labeled with a what?

A

with an identifying case number on the SIDE of the mounted COVERSLIP

64
Q

What is the general rule in labeling slides?

A
  • a PAPER LABEL is attached to the slide for proper identification
65
Q

What must the PAPER LABEL contain in labeling?

A
  1. patient’s name
  2. section number
  3. preferrably the staining method used
66
Q

What can be avoided by using a PAPER LABEL attached to the slide in labeling?

A

to avoid an damage to the section caused by WIPING the “wrong”’ side of the slide

67
Q

What can be avoided by using a PAPER LABEL attached to the slide in labeling?

A

to avoid an damage to the section caused by WIPING the “wrong”’ side of the slide

68
Q

What are the 2 main groups a mounting media may be divided into?

A
  1. Aqueous media
  2. Resinous media

HISTOPATH LEC (7 decks)

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