Mounting And Labelling Flashcards
(18 cards)
A step in histopath that can’t be automated
Mounting
Last step in histopath
Labelling
Term to descirbe the use of a medium and a coverslip to facilitate the handling, storage, protection of the tissue section
Mounting
It is a syrupy fluid to prevemt movement of the coverslip
Mounting medium
Methods of Mounting Sections
- Slide lowered on to the coverslip and quickly inverted
2. Coverslip lowered on to slide
Mounting medium must be close to have a refractive index of a glass which us
1.518
Kinds of mounting medium
- Aqeous
2. Resinous
Composition of aqeous mounting medium
Gelatin, glycerin jelly or gum arabic
Glycerol
Sugar
Preservative
Glycerol’s use in aqeous mounting medium
Prevent drying and cracking
Sugar’s purpose in aqeous mounting medium
Increase refractive index
A tyoe of mounting media used in frozen sections
Aqeous mounting media
What are the aqeous mounting media
Water Glycerin Farrant's medium Apathy's medium Brun's fluid Levulose (fructose)
Resinous mounting mediums
Canada balsam DPX XAM Clarite Permount, HSR, Clearmount
A resinous media for whole mounts and thick sections
Canada balsam
Term used in the sealing of the margins of the coverslip to prevent escape of fluid and evaporation
Ringing
Kinds of ringing media
Kronig cement
Durofix/Du Noyer’s (cellulose adhesives)
It is the process of indicating the year and specimen no. On one end of the prepared slide for proper identification
Labelling
Charavteristics of good adhesive
- Must be permeable to both fixative and the stain
2. Must not retain the stain
