Mounting And Labelling

Question 1

A step in histopath that can’t be automated

Answer 1

Mounting

Question 2

Last step in histopath

Answer 2

Labelling

Question 3

Term to descirbe the use of a medium and a coverslip to facilitate the handling, storage, protection of the tissue section

Answer 3

Mounting

Question 4

It is a syrupy fluid to prevemt movement of the coverslip

Answer 4

Mounting medium

Question 5

Methods of Mounting Sections

Answer 5

1. Slide lowered on to the coverslip and quickly inverted

2. Coverslip lowered on to slide

Question 6

Mounting medium must be close to have a refractive index of a glass which us

Answer 6

1.518

Question 7

Kinds of mounting medium

Answer 7

1. Aqeous

2. Resinous

Question 8

Composition of aqeous mounting medium

Answer 8

Gelatin, glycerin jelly or gum arabic
Glycerol
Sugar
Preservative

Question 9

Glycerol’s use in aqeous mounting medium

Answer 9

Prevent drying and cracking

Question 10

Sugar’s purpose in aqeous mounting medium

Answer 10

Increase refractive index

Question 11

A tyoe of mounting media used in frozen sections

Answer 11

Aqeous mounting media

Question 12

What are the aqeous mounting media

Answer 12

Water
Glycerin
Farrant's medium
Apathy's medium
Brun's fluid
Levulose (fructose)

Question 13

Resinous mounting mediums

Answer 13

Canada balsam
DPX
XAM
Clarite
Permount, HSR, Clearmount

Question 14

A resinous media for whole mounts and thick sections

Answer 14

Canada balsam

Question 15

Term used in the sealing of the margins of the coverslip to prevent escape of fluid and evaporation

Answer 15

Ringing

Question 16

Kinds of ringing media

Answer 16

Kronig cement

Durofix/Du Noyer’s (cellulose adhesives)

Question 17

It is the process of indicating the year and specimen no. On one end of the prepared slide for proper identification

Answer 17

Labelling

Question 18

Charavteristics of good adhesive

Answer 18

1. Must be permeable to both fixative and the stain

2. Must not retain the stain